Analysis of Physical Activity Among Free–Living Nonagenarians From a Sardinian Longevous Population

Physical activity was identified as a major determinant of longevity. Using wearable accelerometers, we evaluated energy expenditure (EE), including resting- (REE) and total-energy expenditure (TEE), physical activity level (PAL), percentage of PAL ≥ 3 metabolic equivalent tasks (METs), number of steps, resting index (RI%) and sleep patterns in 44 free-living nonagenarians (27 men) residing in a Sardinian village famous for its longevous population. The average REE and TEE recorded were 1275 ± 163 kcal/day and 2284 ± 543 in the men and 952 ± 108 kcal/day and 1810 ± 302 in the women, respectively. The average PAL was 1.8, and the percentage of physical activity >3 METs was greater than 40%. A significant negative correlation (p < 0.05) between disability and PAL was found among the women. This study provides evidence that nonagenarians from the longevous population of Sardinia show excellent physical functionality indexes. Their longevity might result, at least in part, from their ability to stay physically fit during aging

Old Age: Definitions, theory, and history of the concept

Byrnes C., Nicholls M, Tommalin G., International Encyclopedia of Social and Behavioral Sciences, Second edition, pp. 10843-1084

Evidence from case–control and longitudinal studies supports associations of genetic variation in APOE, CETP, and IL6 with human longevity

In this study, we investigated 102 single-nucleotide polymorphisms (SNPs) covering the common genetic variation in 16 genes recurrently regarded as candidates for human longevity: APOE; ACE; CETP; HFE; IL6; IL6R; MTHFR; TGFB1; APOA4; APOC3; SIRTs 1, 3, 6; and HSPAs 1A, 1L, 14. In a case-control study of 1,089 oldest-old (ages 92-93) and 736 middle-aged Danes, the minor allele frequency (MAF) of rs769449 (APOE) was significantly decreased in the oldest-old, while the MAF of rs9923854 (CETP) was significantly enriched. These effects were supported when investigating 1,613 oldest-old (ages 95-110) and 1,104 middle-aged Germans. rs769449 was in modest linkage equilibrium (R (2) = 0.55) with rs429358 of the APOE-ε4 haplotype and adjusting for rs429358 eliminated the association of rs769449, indicating that the association likely reflects the well-known effect of rs429358. Gene-based analysis confirmed the effects of variation in APOE and CETP and furthermore pointed to HSPA14 as a longevity gene. In a longitudinal study with 11 years of follow-up on survival in the oldest-old Danes, only one SNP, rs2069827 (IL6), was borderline significantly associated with survival from age 92 (P-corrected = 0.064). This advantageous effect of the minor allele was supported when investigating a Dutch longitudinal cohort (N = 563) of oldest-old (age 85+). Since rs2069827 was located in a putative transcription factor binding site, quantitative RNA expression studies were conducted. However, no difference in IL6 expression was observed between rs2069827 genotype groups. In conclusion, we here support and expand the evidence suggesting that genetic variation in APOE, CETP, and IL6, and possible HSPA14, is associated with human longevity.Molecular Epidemiolog