EKSPLORASI DESAIN MOTIF FLORA DENGAN TEKNIK LUKIS PADA LENAN RUMAH TANGGA

Penelitian ini bertujuan untuk mengetahui hasil penerapan ragam hias flora pada media tekstil menggunakan teknik lukis pada siswa kelas VII SMP Negeri 1 Tilango Kabupaten Gorontalo. Jenis penelitian ini menggunakan metode kualitatif deskriftif. Penentuan sampel menggunakan purposive sampling dengan sampel penelitian adalah siswa kelas VII-1 jumlah 25 siswa. Pengumpulan data melalui observasi, wawancara, dan dokumentasi. Analisis data menggunakan teknik analisis data kualitatif melalui reduksi data, penyajian data, dan verifikasi. Hasil penelitian menunjukkan hal sebagai berikut, yakni proses pelaksanaan pembuatan ragam hias flora pada media tekstil, yang berukuran Panjang 55 cm x Lebar 55 cm dalam bentuk taplak meja, dari 4 kategori penilaian tidak ada siswa yang memperoleh kategori sangat baik (0%), kategori baik diperoleh 12 siswa (48%), kategori cukup diperoleh 13 siswa (52%), dan tidak ada siswa yang memperoleh kategori kurang (0%). Berdasarkan hasil penelitian disimpulkan bahwa penggunaan alternatif media, diharapkan dapat menumbuhkan motivasi siswa dalam berkarya, hal ini dapat dilihat dari seluruh hasil  karya siswa telah mencapai nilai KKM, dan juga hasil karya bisa dimanfaatkan sebagai contoh karya untuk siswa berikutnya, guna meningkatkan kemampuan siswa dalam berkarya ragam hias flora pada media tekstil, sehingga menghasilkan karya yang bernilai fungsional. Kata kunci: Penerapan, Ragam Hias Flora, Tekstil, Luki

Gestão de leitos : desafios no processo de trabalho em um hospital público

Orientador : Laís Carolini Theisrabalho de conclusão de curso (especialização) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Curso de Especialização em Gestão da SaúdeInclui referênciasResumo :O serviço de Gestão de Leitos do Complexo Hospital de Clínicas da Universidade Federal do Paraná (CHC-UFPR) está respaldado pela Política Nacional de Atenção Hospitalar e pela Política Nacional de Regulação do Sistema Único de Saúde (SUS). Foi criado em outubro de 2015 e faz parte do Setor de Regulação. Trata-se de um serviço em construção, que busca a otimização de leitos hospitalares, o qual encontrou alguns desafios desde sua criação. O objetivo deste projeto constitui identificar e analisar os principais problemas encontrados a partir de sua criação até o cenário atual, e propor ações de melhoria no processo de trabalho. A partir da observação e análise dos principais desafios encontrados, foi elaborado um conjunto de ações acessíveis, visando a diminuição da espera pelos leitos, garantindo a acessibilidade e satisfação do usuário. A proposta técnica sugerida para resolução destes problemas está relacionada à ampliação da equipe da gestão de leitos, através do funcionamento do serviço 24 horas. Com o funcionamento do plantão noturno, as transferências e admissões seriam realizadas pela equipe da gestão de leitos sem interrupção, proporcionando maior autonomia para receber os pacientes da rede, maior agilidade nas transferências dos pacientes internos e a liberação mais rápida dos leitos críticos e da emergência

Phylogenomic Analysis of Secondary Metabolism in the Toxic Cyanobacterial Genera Anabaena, Dolichospermum and Aphanizomenon

Cyanobacteria produce an array of toxins that pose serious health risks to humans and animals. The closely related diazotrophic genera, Anabaena, Dolichospermum and Aphanizomenon, frequently form poisonous blooms in lakes and brackish waters around the world. These genera form a complex now termed the Anabaena, Dolichospermum and Aphanizomenon (ADA) clade and produce a greater array of toxins than any other cyanobacteria group. However, taxonomic confusion masks the distribution of toxin biosynthetic pathways in cyanobacteria. Here we obtained 11 new draft genomes to improve the understanding of toxin production in these genera. Comparison of secondary metabolite pathways in all available 31 genomes for these three genera suggests that the ability to produce microcystin, anatoxin-a, and saxitoxin is associated with specific subgroups. Each toxin gene cluster was concentrated or even limited to a certain subgroup within the ADA clade. Our results indicate that members of the ADA clade encode a variety of secondary metabolites following the phylogenetic clustering of constituent species. The newly sequenced members of the ADA clade show that phylogenetic separation of planktonic Dolichospermum and benthic Anabaena is not complete. This underscores the importance of taxonomic revision of Anabaena, Dolichospermum and Aphanizomenon genera to reflect current phylogenomic understanding

Phylogenomic Analysis of Secondary Metabolism in the Toxic Cyanobacterial Genera Anabaena, Dolichospermum and Aphanizomenon

Cyanobacteria produce an array of toxins that pose serious health risks to humans and animals. The closely related diazotrophic genera, Anabaena, Dolichospermum and Aphanizomenon, frequently form poisonous blooms in lakes and brackish waters around the world. These genera form a complex now termed the Anabaena, Dolichospermum and Aphanizomenon (ADA) clade and produce a greater array of toxins than any other cyanobacteria group. However, taxonomic confusion masks the distribution of toxin biosynthetic pathways in cyanobacteria. Here we obtained 11 new draft genomes to improve the understanding of toxin production in these genera. Comparison of secondary metabolite pathways in all available 31 genomes for these three genera suggests that the ability to produce microcystin, anatoxin-a, and saxitoxin is associated with specific subgroups. Each toxin gene cluster was concentrated or even limited to a certain subgroup within the ADA clade. Our results indicate that members of the ADA clade encode a variety of secondary metabolites following the phylogenetic clustering of constituent species. The newly sequenced members of the ADA clade show that phylogenetic separation of planktonic Dolichospermum and benthic Anabaena is not complete. This underscores the importance of taxonomic revision of Anabaena, Dolichospermum and Aphanizomenon genera to reflect current phylogenomic understanding

Genome Reduction and Secondary Metabolism of the Marine Sponge-Associated Cyanobacterium Leptothoe

Sponges form symbiotic relationships with diverse and abundant microbial communities. Cyanobacteria are among the most important members of the microbial communities that are associated with sponges. Here, we performed a genus-wide comparative genomic analysis of the newly described marine benthic cyanobacterial genus Leptothoe (Synechococcales). We obtained draft genomes from Le. kymatousa TAU-MAC 1615 and Le. spongobia TAU-MAC 1115, isolated from marine sponges. We identified five additional Leptothoe genomes, host-associated or free-living, using a phylogenomic approach, and the comparison of all genomes showed that the sponge-associated strains display features of a symbiotic lifestyle. Le. kymatousa and Le. spongobia have undergone genome reduction; they harbored considerably fewer genes encoding for (i) cofactors, vitamins, prosthetic groups, pigments, proteins, and amino acid biosynthesis; (ii) DNA repair; (iii) antioxidant enzymes; and (iv) biosynthesis of capsular and extracellular polysaccharides. They have also lost several genes related to chemotaxis and motility. Eukaryotic-like proteins, such as ankyrin repeats, playing important roles in sponge-symbiont interactions, were identified in sponge-associated Leptothoe genomes. The sponge-associated Leptothoe stains harbored biosynthetic gene clusters encoding novel natural products despite genome reduction. Comparisons of the biosynthetic capacities of Leptothoe with chemically rich cyanobacteria revealed that Leptothoe is another promising marine cyanobacterium for the biosynthesis of novel natural products

Genome Reduction and Secondary Metabolism of the Marine Sponge-Associated Cyanobacterium Leptothoe

Sponges form symbiotic relationships with diverse and abundant microbial communities. Cyanobacteria are among the most important members of the microbial communities that are associated with sponges. Here, we performed a genus-wide comparative genomic analysis of the newly described marine benthic cyanobacterial genus Leptothoe (Synechococcales). We obtained draft genomes from Le. kymatousa TAU-MAC 1615 and Le. spongobia TAU-MAC 1115, isolated from marine sponges. We identified five additional Leptothoe genomes, host-associated or free-living, using a phylogenomic approach, and the comparison of all genomes showed that the sponge-associated strains display features of a symbiotic lifestyle. Le. kymatousa and Le. spongobia have undergone genome reduction; they harbored considerably fewer genes encoding for (i) cofactors, vitamins, prosthetic groups, pigments, proteins, and amino acid biosynthesis; (ii) DNA repair; (iii) antioxidant enzymes; and (iv) biosynthesis of capsular and extracellular polysaccharides. They have also lost several genes related to chemotaxis and motility. Eukaryotic-like proteins, such as ankyrin repeats, playing important roles in sponge-symbiont interactions, were identified in sponge-associated Leptothoe genomes. The sponge-associated Leptothoe stains harbored biosynthetic gene clusters encoding novel natural products despite genome reduction. Comparisons of the biosynthetic capacities of Leptothoe with chemically rich cyanobacteria revealed that Leptothoe is another promising marine cyanobacterium for the biosynthesis of novel natural products

Clozapine-Induced Pericarditis

Objective: To report a case of a patient treated with clozapine who developed pericarditis with pericardial effusion that resolved when the drug was discontinued. Method: Case report of a 21-year-old man with psychotic disorder that had been stable on clozapine therapy for five months (after failure of atypical antipsyhotic agents) presented to the emergency department complaining of chest pain and progressive shortness of breath that had lasted for a few days. Echocardiography showed a pericardial effusion suggestive of a cardiac tamponade, and the fluid was removed by pericardiocentesis. All other possible causes of the pericardial effusion were ruled out and clozapine was suspected as the most likely explanation. Clozapine was discontinued and the patient’s symptoms improved markedly. Discussion: According to the Naranjo probability scale, clozapine is a probable cause of pericarditis. Although clozapine is a known cause of myocarditis and cardiomyopathy, there are only several reports in the literature describing clozapine-induced pericarditis and pericardial effusion. In our patient, the pericardial effusion cleared within several days following clozapine discontinuation. Conclusion: There have been only a few cases of clozapine-induced pericarditis reported in the literature, however this adverse effect of clozapine can occur, as this case report clearly  demonstrates. Cardiac adverse effects of clozapine are potentially life threatening, hence early recognition is essential to prevent serious outcomes.Key words: Clozapine; Pericarditis; Pericardial effusion; Adverse reactio

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Cyanobacteria are photosynthetic organisms that produce a large diversity of natural products with interesting bioactivities for biotechnological and pharmaceutical applications. Cyanobacterial extracts exhibit toxicity towards other microorganisms and cancer cells and, therefore, represent a source of potentially novel natural products for drug discovery. We tested 62 cyanobacterial strains isolated from various Brazilian biomes for antileukemic and antimicrobial activities. Extracts from 39 strains induced selective apoptosis in acute myeloid leukemia (AML) cancer cell lines. Five of these extracts also exhibited antifungal and antibacterial activities. Chemical and dereplication analyses revealed the production of nine known natural products. Natural products possibly responsible for the observed bioactivities and five unknown, chemically related chlorinated compounds present only in Brazilian cyanobacteria were illustrated in a molecular network. Our results provide new information on the vast biosynthetic potential of cyanobacteria isolated from Brazilian environments.Peer reviewe

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Cyanobacteria are photosynthetic organisms that produce a large diversity of natural products with interesting bioactivities for biotechnological and pharmaceutical applications. Cyanobacterial extracts exhibit toxicity towards other microorganisms and cancer cells and, therefore, represent a source of potentially novel natural products for drug discovery. We tested 62 cyanobacterial strains isolated from various Brazilian biomes for antileukemic and antimicrobial activities. Extracts from 39 strains induced selective apoptosis in acute myeloid leukemia (AML) cancer cell lines. Five of these extracts also exhibited antifungal and antibacterial activities. Chemical and dereplication analyses revealed the production of nine known natural products. Natural products possibly responsible for the observed bioactivities and five unknown, chemically related chlorinated compounds present only in Brazilian cyanobacteria were illustrated in a molecular network. Our results provide new information on the vast biosynthetic potential of cyanobacteria isolated from Brazilian environments.Peer reviewe