JOIA DE TERRITÓRIO NO ESTADO DO PARÁ: UMA ANÁLISE DA CADEIA PRODUTIVA DOS ADORNOS COM SEMENTES NATIVAS: TERRITORY JEWEL IN THE STATE OF PARÁ: AN ANALYSIS OF THE PRODUCTIVE CHAIN OF ADORNMENT WITH NATIVE SEEDS

This paper presents the partial results of the master's research developed at the UFPE's PPGDesign, whose objective was to map the productive chain of territory jewelry from Pará with Amazonian seeds, aiming to contribute to the strengthening and sustainability of the local jewelry sector. The method was adapted from the Handicraft Productive Chain Analysis Model proposed by O Imaginário Design Laboratory/UFPE, to collect information for identifying raw materials, production processes and access to the market. The qualitative research had the voluntary participation of designers registered in the Polo Joalheiro Program/Pará; representatives of IGAMA and Embrapa, with the first results indicative of weaknesses in the initial manufacturing stages. Producers depend on middlemen to collect and process raw materials, however, Embrapa-PA already has an effective method for seed treatment, which has not yet been applied by local producers.Este artículo presenta los resultados parciales de la investigación de maestría desarrollada en el PPGDesign de la UFPE, cuyo objetivo fue mapear la cadena productiva de la joyería del territorio de Pará con semillas amazónicas para contribuir al fortalecimiento y sostenibilidad del sector joyero local. El método fue adaptado del Modelo de Análisis de la Cadena Productiva Artesanal propuesto por el Laboratorio de Diseño O Imaginário/UFPE, para recoger información sobre la identificación de materias primas, procesos productivos y acceso al mercado. La investigación cualitativa contó con la participación voluntaria de diseñadores inscritos en el Programa Polo Joyería/Pará; representantes del IGAMA y de Embrapa, y los primeros resultados indican debilidades en las etapas iniciales de la fabricación. Los productores dependen de intermediarios para recolectar y procesar la materia prima, sin embargo, Embrapa-PA ya cuenta con un método eficaz para el tratamiento de las semillas, aún no practicado por los productores locales.Este artigo apresenta os resultados parciais da pesquisa de mestrado desenvolvida no PPGDesign da UFPE, cujo objetivo foi mapear a cadeia produtiva das joias de território do Pará com sementes amazônicas visando contribuir para o fortalecimento e a sustentabilidade do setor joalheiro local. O método foi adaptado do Modelo de Análise da Cadeia Produtiva do Artesanato proposto pelo Laboratório de Design O Imaginário/UFPE, para coletar informações sobre a identificação da matéria-prima, dos processos produtivos e do acesso ao mercado. A pesquisa de cunho qualitativo contou com a participação voluntária de designers cadastrados no Programa Polo Joalheiro/Pará; representantes do IGAMA e da Embrapa, sendo os primeiros resultados, indicativos de fragilidades nas etapas iniciais de fabricação. Os produtores dependem dos atravessadores para coleta e beneficiamento da matéria prima, no entanto, a Embrapa-PA já dispõe de um método eficaz para o tratamento das sementes, ainda não praticado pelos produtores locais

JOIA DE TERRITÓRIO NO ESTADO DO PARÁ: UMA ANÁLISE DA CADEIA PRODUTIVA DOS ADORNOS COM SEMENTES NATIVAS: TERRITORY JEWEL IN THE STATE OF PARÁ: AN ANALYSIS OF THE PRODUCTIVE CHAIN OF ADORNMENT WITH NATIVE SEEDS

This paper presents the partial results of the master's research developed at the UFPE's PPGDesign, whose objective was to map the productive chain of territory jewelry from Pará with Amazonian seeds, aiming to contribute to the strengthening and sustainability of the local jewelry sector. The method was adapted from the Handicraft Productive Chain Analysis Model proposed by O Imaginário Design Laboratory/UFPE, to collect information for identifying raw materials, production processes and access to the market. The qualitative research had the voluntary participation of designers registered in the Polo Joalheiro Program/Pará; representatives of IGAMA and Embrapa, with the first results indicative of weaknesses in the initial manufacturing stages. Producers depend on middlemen to collect and process raw materials, however, Embrapa-PA already has an effective method for seed treatment, which has not yet been applied by local producers.Este artículo presenta los resultados parciales de la investigación de maestría desarrollada en el PPGDesign de la UFPE, cuyo objetivo fue mapear la cadena productiva de la joyería del territorio de Pará con semillas amazónicas para contribuir al fortalecimiento y sostenibilidad del sector joyero local. El método fue adaptado del Modelo de Análisis de la Cadena Productiva Artesanal propuesto por el Laboratorio de Diseño O Imaginário/UFPE, para recoger información sobre la identificación de materias primas, procesos productivos y acceso al mercado. La investigación cualitativa contó con la participación voluntaria de diseñadores inscritos en el Programa Polo Joyería/Pará; representantes del IGAMA y de Embrapa, y los primeros resultados indican debilidades en las etapas iniciales de la fabricación. Los productores dependen de intermediarios para recolectar y procesar la materia prima, sin embargo, Embrapa-PA ya cuenta con un método eficaz para el tratamiento de las semillas, aún no practicado por los productores locales.Este artigo apresenta os resultados parciais da pesquisa de mestrado desenvolvida no PPGDesign da UFPE, cujo objetivo foi mapear a cadeia produtiva das joias de território do Pará com sementes amazônicas visando contribuir para o fortalecimento e a sustentabilidade do setor joalheiro local. O método foi adaptado do Modelo de Análise da Cadeia Produtiva do Artesanato proposto pelo Laboratório de Design O Imaginário/UFPE, para coletar informações sobre a identificação da matéria-prima, dos processos produtivos e do acesso ao mercado. A pesquisa de cunho qualitativo contou com a participação voluntária de designers cadastrados no Programa Polo Joalheiro/Pará; representantes do IGAMA e da Embrapa, sendo os primeiros resultados, indicativos de fragilidades nas etapas iniciais de fabricação. Os produtores dependem dos atravessadores para coleta e beneficiamento da matéria prima, no entanto, a Embrapa-PA já dispõe de um método eficaz para o tratamento das sementes, ainda não praticado pelos produtores locais

The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models

We present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilLNBio CNPEM, Lab Nacl Biociencias, Campinas, SP, BrazilLGE UNICAMP, Lab Genom & Expressao, Campinas, SP, BrazilInst Agron Campinas, Ctr Pesquisa & Desenvolvimento Recursos Geneti Ve, Campinas, SP, BrazilUniv Calif San Diego, Sch Med, Dept Pediat, San Diego, CA 92103 USAUniversidade Federal de São Paulo, UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, BrazilUniv N Carolina, Sch Med, Dept Genet, Chapel Hill, NC USAUniv Fed Minas Gerais, ICB UFMG, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, BrazilFAPESP: 07/50551-2FAPESP: 10/19335-4Web of Scienc

A joia de território do estado do Pará com sementes amazônicas: estratégias para a sustentabilidade do setor a partir da análise da cadeia produtiva

This paper presents the partial results of the master's research developed at the UFPE's PPGDesign, whose objective was to map the productive chain of territory jewelry from Pará with Amazonian seeds, aiming to contribute to the strengthening and sustainability of the local jewelry sector. The method was adapted from the Handicraft Productive Chain Analysis Model proposed by O Imaginário Design Laboratory/UFPE, to collect information for identifying raw materials,production processes and access to the market. The qualitative research had the voluntary participation of designers registered in the Polo Joalheiro Program/Pará; representatives of IGAMA and Embrapa, with the first results indicative of weaknesses in the initial manufacturing stages. Producers depend on middlemen to collect and process raw materials, however, Embrapa-PA already has an effective method for seed treatment, which has not yet been applied by local producers

The preliminary design of a scaled Composite UHBR Fan for a wind tunnel test campaign

AbstractThe ambition of the CA3ViAR project is to design an open test case fan that experiences instability mechanisms, which are representative for ultra-high bypass ratio (UHBR) fans of civil aircrafts, and to perform a comprehensive experimental investigation to measure aerodynamic, aeroelastic and aeroacoustic performance in a wide range of operational conditions. Experimental tests will be performed in the Propulsion-Test-Facility (PTF) of the Institute of Jet Propulsion and Turbomachinery (IFAS) of Technische Universität Braunschweig, Germany. The final objective of the project is to provide an open test case for the entire research community, with geometries, numerical and experimental results to establish a new reference for composite UHBR fan design. This will support the development of new methods and tools for the development of safer, lighter and more efficient composite fans for greener UHBR engines. In this work the preliminary design of the low transonic fan (LTF) to be used as test article, whose main requirement is to be operated in a safe and controlled way in conditions of aerodynamic and/or aeroelastic instability during wind tunnel operations, is presented. More in particular, consolidated aerodynamic design, strategy adopted to drive the structural design, flutter analysis taking into account acoustic reflection at the intake, dynamic and stress analyses, as well as aeroacoustic measurement optimization are presented and discussed. The preliminary mechanical design of composite blades and the rotor hub, together with the rotor instrumentation and related studies to embed sensors in the composite blades, are also part of this article, and complemented by manufacturing trials and demonstration tests give the full picture of all the project activities up to the preliminary design review

Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells

The aim of this study was to characterize synthetic poly-(L-lactic acid) (PLLA) nanofibers concerning their ability to promote growth and osteogenic differentiation of stem cells in vitro, as well as to test their suitability as a carrier system for growth factors. Fiber matrices composed of PLLA or BMP-2–incorporated PLLA were seeded with human mesenchymal stem cells and cultivated over a period of 22 days under growth and osteoinductive conditions, and analyzed during the course of culture, with respect to gene expression of alkaline phosphatase (ALP), osteocalcin (OC), and collagen I (COL-I). Furthermore, COL-I and OC deposition, as well as cell densities and proliferation, were analyzed using fluorescence microscopy. Although the presence of nanofibers diminished the dexamethasone-induced proliferation, there were no differences in cell densities or deposition of either COL-I or OC after 22 days of culture. The gene expression of ALP, OC, and COL-I decreased in the initial phase of cell cultivation on PLLA nanofibers as compared to cover slip control, but normalized during the course of cultivation. The initial down-regulation was not observed when BMP-2 was directly incorporated into PLLA nanofibers by electrospinning, indicating that growth factors like BMP-2 might survive the spinning process in a bioactive form

Redox-responsive branched-bottlebrush polymers for \u3ci\u3ein vivo\u3c/i\u3e MRI and fluorescence imaging

Stimuli-responsive multimodality imaging agents have broad potential in medical diagnostics. Herein, we report the development of a new class of branched-bottlebrush polymer dual-modality organic radical contrast agents—ORCAFluors—for combined magnetic resonance and near-infrared fluorescence imaging in vivo. These nitroxide radical-based nanostructures have longitudinal and transverse relaxation times that are on par with commonly used heavy-metal-based magnetic resonance imaging (MRI) contrast agents. Furthermore, these materials display a unique compensatory redox response: fluorescence is partially quenched by surrounding nitroxides in the native state; exposure to ascorbate or ascorbate/glutathione leads to nitroxide reduction and a concomitant 2- to 3.5-fold increase in fluorescence emission. This behaviour enables correlation of MRI contrast, fluorescence intensity and spin concentration with tissues known to possess high concentrations of ascorbate in mice. Our in vitro and in vivo results, along with our modular synthetic approach, make ORCAFluors a promising new platform for multimodality molecular imaging