Razvoj in vitro preantralnih folikula u uzgoju s kumulusnim ili granuloznim stanicama.

Low reproductive effifi ciency in buffalo limits the number of offspring produced during lifespan and thus results in under-exploitation of the superior female genetic material. In vitro production of transferable embryos is a method used to overcome such limitation. Developing a culture system for preantral follicles has important biotechnological implications, due to its potential for producing large number of oocytes for in vitro embryo production. Hence, the effect of the co-culture of buffalo preantral follicles with dispersed somatic cells, like cumulus cells and granulosa cells and their monolayers, was investigated in the present study. Large preantral follicles were isolated from trypsin (1%) digested ovarian cortical slices. Cumulus and granulosa cell monolayers were prepared by culturing cells until their conflfl uency preantral follicles were co-cultured with dispersed cumulus cells (group 1), the monolayers of cumulus cells (group 2), dispersed granulosa cells (group 3) or the monolayers of granulosa cells (group 4). Large preantral follicles showed signififi cantly higher size, growth rate and survivability when co-cultured with somatic cells dispersed in the medium, as compared to those grown in a monolayer. It was concluded that dispersed somatic cells from buffalo ovarian follicles were more effective in providing support for the growth and survivability of preantral follicles in culture, compared to their monolayers in buffalo.Niska rasplodna sposobnost bivolica ograničava broj potomaka podrijetlom od jedne bivolice tijekom njezina životnoga vijeka, a time i iskorištavanje velikih mogućnosti ženskoga genetskoga materijala. Proizvodnja zametaka in vitro u svrhu prijenosa u plotkinju metoda je koja može premostiti to ograničenje. Razvoj tehnologije uzgoja preantralnih folikula od važnoga je biotehnološkog značenja zahvaljujući mogućnostima proizvodnje velikoga broja jajašaca radi proizvodnje zametaka in vitro. Stoga je istraživan učinak suuzgoja bivoljih antralnih folikula s raspršenim somatskim stanicama kao što su kumulusne i granulozne stanice i njihovim jednoslojnim kulturama. Veliki preantralni folikuli bili su izdvojeni iz komadića kore jajnika u postupku probave 1%-tnim tripsinom. Kumulusne i granulozne stanice bile su uzgajane sve dok se nisu spojile u jednom sloju. Preantralni folikuli bili su uzgajani zajedno s raspršenim kumulusnim stanicama (skupina 1), kumulusnim stanicama uzgojenima u jednom sloju (skupina 2), raspršenim granuloznim stanicama (skupina 3) i granuloznim stanicama uzgojenima u jednom sloju (skupina 4). Veliki preantralni folikuli bili su značajno veći, brže su rasli i duže preživljavali u suuzgoju sa somatskim stanicama raspršenima u hranjivoj tekućini nego u suuzgoju sa stanicama naraslima u jednom sloju. Zaključeno je da su raspršene somatske stanice podrijetlom od jajničnoga folikula bivolice bile učinkovitija podloga za rast i preživljavanje preantralnih folikula od uzgojenih u jednom sloju

Upaya Orang Tua untuk Meningkatkan Prestasi Belajar Siswa Kelas Vi SD Tahun Ajaran 1997/1998 (Studi Kasus Terhadap Murid SD Kelas Vi Kotamadya Palu)

Penelitian ini bertujuan untuk menjawab permasalahan yang terdapat pada rumusan masalah yaitu mendeskripsikan (1) upaya-upaya yang dilakukan orang tua untuk meningkatkan prestasi belajar siswa kelas VI SD Kotamadya Palu, dan (2) untuk memperoleh gambaran yang obyektif tentang hubungan yang signifikan antara upaya orang tua dengan prestasi belajar siswa kelas VI SD di Kotamadya Palu

Membangun Kesadaran Kritis Petani Dalam Mewujudkan Pertanian Berkelanjutan Melalui Penggunaan Pupuk Organik Di Desa Karya Mukti, Sulawesi Tengah

Pertanian di Desa Karya Mukti menggunakan pupuk kimia terus menerus, karena belum adanya pengetahuan masyarakat tentang dampak penggunaan pupuk kimia dan manfaat pupuk organik bagi tanaman. Program pengabdian kepada masyarakat ini diawali dengan memberikan informasi mengenai pentingnya pupuk organik dan dilanjutkan dengan pelatihan pembuatan pupuk organik. Kegiatan pengabdian kepada masyarakat ini diharapkan dapat mengembangkan Kelompok Tani Subak Anyar yang ada di Desa Karya Mukti sebagai desa yang mampu menyediakan pupuk organik secara mandiri dan menjadi contoh bagi kelompok tani lain untuk mewujudkan pertanian ramah lingkungan dan meminimalkan biaya pembelian pupuk kimia

Knowledge, attitude, and preventive practices of leptospirosis affected populations in South Andaman, India: A cross-sectional study

Introduction: Leptospirosis is the most common reoccurring zoonosis worldwide. Climatic conditions in tropical and subtropical regions are optimal for Leptospira survival. The pathogen thrives in flood-prone slum settlements of underprivileged areas where waste, open sewers, and standing water are present. Methods: A descriptive cross-sectional study using universal sampling methodology was conducted to determine associationsbetween sociodemographic variables and knowledge, attitudes, and practices of leptospirosis-infected individuals compared with a control group from the South Andaman population. Results: Eight hundred and one (388 cases and 413 controls) subjects were included in the study. Overall, 61.5% of the participants were male, while the main occupation of 43.94% of the subjects was farming or agricultural work. Multilogistic regression assessing the likelihood of good knowledge about leptospirosis showed that leptospirosis-positive subjects were more likely to have good knowledge (adjusted odds ratio [AOR]: 3.5 [95% CI: 2.59–4.97], p < 0.001), better attitude (AOR: 97.30 [95% CI: 41.72–226.9], p < 0.001] than leptospirosis-negative subjects, male population groups were also more likely to have a good attitude (AOR: 3.03 [95% CI: 1.94–4.73], p < 0.001), and those whose main occupation is farming were more likely to have a good attitude (AOR: 3.59 [95% CI: 2.31–5.56], p < 0.001). The leptospirosis seropositive group was more likely to have good practices (AOR: 5.80 [95% CI: 3.58–8.73], p < 0.001), rural residents were 88% less likely to have good practice levels than urban residents (AR: 0.12 [95% CI: 0.07–0.20], p < 0.01). Conclusion: The infected group had better knowledge than the control group. The integration of knowledge and attitudes to maintain good practices, along with the provision of an adequate sanitation system, waste disposal system, and availability of essential personal protective equipment is necessary for disease control in these islands

Brainstem Auditory Evoked Potentials in Type 2 Diabetes Mellitus

Diabetes mellitus is a group of metabolic diseases characterized by hyperglycaemia resulting from defects in insulin secretion, insulin action or both. Diabetes affects many systems and produces complications in the human body, in those complications one is diabetic central neuropathy. The pathological mechanisms involved in the central neuropathy include chronic hyperglycaemia, hypoglycaemic episodes, angiopathy and blood–brain barrier dysfunction. Diabetic central neuropathy is detected by using of brainstem auditory evoked response (BAER), Visual evoked potential (VEP), somatosensory evoked potential (SEP). These abnormalities are present at different levels and may appear before appearance of overt complications. The central nervous system abnormalities are more frequent in patients with peripheral neuropathy but evoked potentials can be abnormal even in patients without neuropathy. The BAER is a physiological recording technique to study the auditory pathway and does not require subject’s attention and generates waves during the first 10 ms after the sound stimulus. Each BAER wave is generated by the activation of a sub-cortical component of the auditory pathway with 90% sensitivity and 70–90% of specificity

Design and Implementation of Deep Learning Based Contactless Authentication System Using Hand Gestures

Hand gestures based sign language digits have several contactless applications. Applications include communication for impaired people, such as elderly and disabled people, health-care applications, automotive user interfaces, and security and surveillance. This work presents the design and implementation of a complete end-to-end deep learning based edge computing system that can verify a user contactlessly using ‘authentication code’. The ‘authentication code’ is an ‘n’ digit numeric code and the digits are hand gestures of sign language digits. We propose a memory-efficient deep learning model to classify the hand gestures of the sign language digits. The proposed deep learning model is based on the bottleneck module which is inspired by the deep residual networks. The model achieves classification accuracy of 99.1% on the publicly available sign language digits dataset. The model is deployed on a Raspberry pi 4 Model B edge computing system to serve as an edge device for user verification. The edge computing system consists of two steps, it first takes input from the camera attached to it in real-time and stores it in the buffer. In the second step, the model classifies the digit with the inference rate of 280 ms, by taking the first image in the buffer as input.publishedVersio

Atypical presentation of Hand foot mouth disease (HFMD) caused by enterovirus serotype Coxsackievirus A6, in India

A 27-year-old male presented in the OPD of Naval Hospital in Port Blair, Andaman Islands, India, in 2011 with a history of low-grade fever associated with malaise and a pruritic skin rash. Case 2 – A 17-year-old male student reported to the OPD at Naval Hospital, Kochi Kerala, India, in August 2015. He presented with eruptions on both the palm and soles with a history of high-grade fever for the past 3–4 days. Clinically, both the cases were diagnosed as hand, foot, and mouth disease (HFMD). Both samples were tested against measles virus and varicella-zoster IgM antibodies by enzyme immunoassay and found negative. Stool sample (case 1) and lesion swab (case 2) were processed by enterovirus reverse transcription polymerase chain reaction and phylogenetic analysis, and both were positive for enterovirus human coxsackievirus A6 (CVA6) (untranslated region [UTR]). Phylogenetic analysis also confirmed that both the CVA6 etiology belonged to the genotype F. HFMD in adults often asymptomatic and very few patients get atypical symptoms. Clinical diagnosis is often troublesome to identify HFMD in such cases. An epidemiological surveillance/vigilance is essential to document these atypical cases in near future in developing countries like India