Analisa Pressure Build Up Test Menggunakan Software Ecrin Dan Metode Manual Pada Sumur Gas Cp-52 & Cp-54 Lapangan Chapin

The CP-52 and CP-54 wells are located on the Tambun structure of Bekasi Regency of West Java Province with the target formation being Baturaja. The pressure build up analysis is carried out for monitoring activities to obtain reservoir and boundary, innitial pressure (Pi), permeability (k), skin (s), ΔP skin, average pressure (Pavg), and flow efficiency information. Tests performed on both wells are pressure build up test (PBU) and to analyze the method of Horner approach pseudo pressure and P2 either manually or with the help of software v.ex.2 v2.02 as validation

Computational protein profile similarity screening for quantitative mass spectrometry experiments

Motivation: The qualitative and quantitative characterization of protein abundance profiles over a series of time points or a set of environmental conditions is becoming increasingly important. Using isobaric mass tagging experiments, mass spectrometry-based quantitative proteomics deliver accurate peptide abundance profiles for relative quantitation. Associated data analysis workflows need to provide tailored statistical treatment that (i) takes the correlation structure of the normalized peptide abundance profiles into account and (ii) allows inference of protein-level similarity. We introduce a suitable distance measure for relative abundance profiles, derive a statistical test for equality and propose a protein-level representation of peptide-level measurements. This yields a workflow that delivers a similarity ranking of protein abundance profiles with respect to a defined reference. All procedures have in common that they operate based on the true correlation structure that underlies the measurements. This optimizes power and delivers more intuitive and efficient results than existing methods that do not take these circumstances into account. Results: We use protein profile similarity screening to identify candidate proteins whose abundances are post-transcriptionally controlled by the Anaphase Promoting Complex/Cyclosome (APC/C), a specific E3 ubiquitin ligase that is a master regulator of the cell cycle. Results are compared with an established protein correlation profiling method. The proposed procedure yields a 50.9-fold enrichment of co-regulated protein candidates and a 2.5-fold improvement over the previous method

The Organ Works of George Whitefield Chadwick (Massachusetts).

George Whitefield Chadwick was one of the most prominent composers in America during the late nineteenth and early twentieth centuries. His organ works included didactic works called studies, several short pieces, and two large concert works, both in variation form. These pieces are suitable for teaching, for service playing, and for recitals. The goal of the study was to isolate the distinguishing stylistic features of Chadwick\u27s organ music including his harmonic language, use of formal structures and melodic material, to examine the specifications of the organs Chadwick knew or might have known, and to determine the influence these instruments had on his organ works. The specifications of four organs that Chadwick played were also included. Chadwick\u27s organ works were found to be a versatile group of pieces offering a variety of sounds and styles. Chadwick retained a conservative idiom throughout all his organ works, although the later pieces seemed to exhibit more chromatic voice leading, more chromatic non-harmonic tones, and distant key relationships. The study concluded with an examination of Chadwick\u27s organ music from a practical perspective, evaluating registration, pedagogical use, and performance suitability. An evaluation of the difficulty of each work was presented in the Appendix

Posttranslational processing of concanavalin A precursors in jackbean cotyledons

Metabolic labeling of immature jackbean cotyledons with 14C-amino acids was used to determine the processing steps involved in the assembly of concanavalin A. Pulse-chase experiments and analyses of immunoprecipitated lectin forms indicated a complex series of events involving seven distinct species. The structural relatedness of all of the intermediate species was confirmed by two-dimensional mapping of 125I-tryptic peptides. An initial glycosylated precursor was deglycosylated and cleaved into smaller polypeptides, which subsequently reannealed over a period of 10-27 h. NH2-terminal sequencing of the abundant precursors confirmed that the intact subunit of concanavalin A was formed by the reannealing of two fragments, since the alignment of residues 1-118 and 119-237 was reversed in the final form of the lectin identified in the chase and the precursor first labeled. When the tissue was pulse-chased in the presence of monensin, processing of the glycosylated precursor was inhibited. The weak bases NH4Cl and chloroquine were without effect. Immunocytochemical studies showed that monensin treatment caused the accumulation of immunoreactive material at the cell surface and indicated that the ionophore had induced the secretion of a component normally destined for deposition within the protein bodies. Consideration of the tertiary structure of the glycosylated precursor and mature lectin showed that the entire series of processing events could occur without significant refolding of the initial translational product. Proteolytic events included removal of a peptide from the surface of the precursor molecule that connected the NH2- and COOH-termini of the mature protein. This processing activated the carbohydrate-binding activity of the lectin. The chase data suggest the occurrence of a simultaneous cleavage and formation of a peptide bond, raising the possibility that annealment of the fragments to give rise to the mature subunit involves a transpeptidation event rather than cleavage and subsequent religation

The Design and Construction of a Fast Track 16 Hectare, 18 m Deep Basement in Soft Clay in Singapore

Singapore’s newest integrated resort, Marina Bay Sands, was completed in record time and has garnered numerous engineering awards. The development sits on recent sand reclamation, which in turn rests on deep soft marine clay deposits. With an average excavation depth of around 18 meters, the 16 hectare (39 acre) waterfront development involved some of the largest marine clay excavation in Singapore. About 2.8 million cubic meters of fill and marine clay were excavated from the site equating to about 800 trucks a day for two years. To overcome the challenges of the bulk excavation and minimize shoring in difficult soil environments, innovative excavation solutions were developed to enable an accelerated construction timetable for this project involving densely packed site works with complex staging and interface issues. These included the use of unsupported circular excavations up to 130 meters in diameter and continuously reinforced 1.5 meter thick diaphragm walls acting in shear. To add to the challenge, a 35 meter deep ‘cut and cover’ tunnel next to the Singapore’s longest bridge, the Benjamin Sheares Bridge, was required. To enable the bridge to tolerate the inevitable imposed lateral displacements of an abutment, the structural system of the existing bridge was modified to allow it to safely articulate in plan

ACT BAD BUT KEEP IT RESPECTABLE: HOW RESPECTABILITY INFLUENCED MY PROFESSIONAL IDENTITY

This autoethnographic study explored how culture and upbringing influenced my approach to respectability politics professionally, the coping strategies I used to deal with microaggressions regarding respectability politics in the workplace, and how the demands of respectability expectations affected my personal life. This autoethnographic study also explored how the normalizing influence of the dominant culture played a role in my professional identity development. It used personal narratives and reflections to examine how these expectations shaped professional identity, coping strategies, and the emotional and physical toll of workplace respectability politics. This study contributes to current research on respectability expectations in professional spaces. The implications of my research contribute to academic, professional, and social realms while also offering contributions to hip-hop feminist theory, Black feminist thought, and human resource policies. The study applies bringing wreck in hip-hop feminism (Pough, 2004) to corporate spaces, extends Black feminist thought’s concept of self-definition (Collins, 1990) to corporate spaces, and addresses how microaggressions in the office can adversely impact wellness. Keywords: Blackgirl, the culture of dissemblance, fucking with the grays, hegemony, identity shifting, intersectionality, microaggression, respectability politics, strong Black woman, stereotype threat, tokenism

Insights into the regulation of DMSP synthesis in the diatom Thalassiosira pseudonana through APR activity, proteomics and gene expression analyses on cells acclimating to changes in salinity, light and nitrogen

Despite the importance of dimethylsulphoniopropionate (DMSP) in the global sulphur cycle and climate regulation, the biological pathways underpinning its synthesis in marine phytoplankton remain poorly understood. The intracellular concentration of DMSP increases with increased salinity, increased light intensity and nitrogen starvation in the diatom Thalassiosira pseudonana. We used these conditions to investigate DMSP synthesis at the cellular level via analysis of enzyme activity, gene expression and proteome comparison. The activity of the key sulphur assimilatory enzyme, adenosine 5′- phosphosulphate reductase was not coordinated with increasing intracellular DMSP concentration. Under all three treatments coordination in the expression of sulphur assimilation genes was limited to increases in sulphite reductase transcripts. Similarly, proteomic 2D gel analysis only revealed an increase in phosphoenolpyruvate carboxylase following increases in DMSP concentration. Our findings suggest that increased sulphur assimilation might not be required for increased DMSP synthesis, instead the availability of carbon and nitrogen substrates may be important in the regulation of this pathway. This contrasts with the regulation of sulphur metabolism in higher plants, which generally involves upregulation of several sulphur assimilatory enzymes. In T. pseudonana changes relating to sulphur metabolism were specific to the individual treatments and, given that little coordination was seen in transcript and protein responses across the three growth conditions, different patterns of regulation might be responsible for the increase in DMSP concentration seen under each treatment

Membrane proteins and proteomics: Love is possible, but so difficult

Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with different proteins, while most protein chemistry methods work mainly, if not only, in water-based media. Taking this review [Santoni, Molloy and Rabilloud, Membrane proteins and proteomics: un amour impossible? Electrophoresis 2000, 21, 1054-1070] as a pivotal paper, the current paper analyzes how the field of membrane proteomics exacerbated the trend in proteomics, i.e. developing alternate methods to the historical two-dimensional electrophoresis, and thus putting more and more pressure on the mass spectrometry side. However, in the case of membrane proteins, the incentive in doing so is due to the poor solubility of membrane proteins. This review also shows that in some situations, where this solubility problem is less acute, two-dimensional electrophoresis remains a method of choice. Last but not least, this review also critically examines the alternate approaches that have been used for the proteomic analysis of membrane proteins