Levering proteomic analysis of Pseudomonas fluorescens mediated resistance responses in tomato during pathogenicity of Fusarium oxysporum f. sp. oxysporum

The tomato, one of the world’s most extensively cultivated and consumed vegetable crops is negatively impacted by various pathogens. This study aimed to observe the differentially expressed proteins in tomato samples in plant–pathogen-biocontrol interactions. The fungal pathogen associated with wilted plants were isolated and identified based on its morphological and molecular characteristics. Fourteen strains of Pseudomonas fluorescens from agricultural soils were identified and described using biochemical assays, molecular analyses, and screening for antagonistic ability against the Fusarium wilt pathogen. Results demonstrated that the potential of P. fluorescens (TPf12) positively influenced the expression of antagonism against tomato wilt disease. A total of 14 proteins expressed differently were revealed in the 2D-PAGE-MS investigation. Proteins such as nucleoside diphosphate kinase, phenylalanine ammonia-lyase, protein kinase family protein, Ser/Thr protein kinase-like are unchanged in FOL pathogen interaction, but up-regulated in FOL + TPf12 treated roots, and lipid transfer-like protein, and phenylalanine ammonia-lyase were down-regulated in FOL infested roots and upregulated in FOL + TPf12 treated tomato roots. Phenylalanine ammonia-lyase protein expression is commonly found in TPf12 bioenriched roots, and FOL + TPf12 treated roots, indicating its role in response to the application of TPf12 in tomato. A GC–MS analysis was performed to detect the bioactive metabolites synthesized by TPf12. Molecular docking investigations were conducted using the maestro’s GLIDE docking module of the Schrodinger Software program. Among the secondary metabolites, Cyclohexanepropanoic acid, 2-oxo-, methyl ester (CAS), and 3-o-(4-o-Beta-D-Galactopyranosyl-Beta-D-Galactopyraosyl)-2-Acetylamino-2-Deoxy-D-Galactose were shown to be top-ranked with a least docking score against each differently expressed proteins. The profiled molecules expressed differently due to plant-pathogen-biocontrol interactions may be directly or incidentally involved in the wilt disease resistance of tomato plants

Characterization of Antifungal Volatile Organic Compounds Produced by Bacterial Endophytes against Fusarium oxysporum through GC-MS Analysis

Banana (Musa spp.) is one of the most important fruit crops cultivated throughout the world. Fusarium wilt caused by the fungus, Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyder &amp; Hansen (Foc) causes significant threats to banana production. Endophytic bacteria play a significant role in plant protection against soil borne plant pathogens and promote overall productivity of agricultural ecosystems. Secondary metabolites produced by several species of Bacillus have been found to possess antibacterial or antifungal activity against different isolates of Fusarium oxysporum f. sp. cubense.  These were identified by crude tests using agar gel diffusion and GC-MS analysis of the fungal crude extracts. Understanding of the metabolites produced by endophytes is also important in the context of biological control of soil-borne diseases of banana. GC-MS analysis of crude antibiotic extracts of bacterial endophytic isolates viz., GNBS3, PVBS3, NPBS4, KVBS4, BS1, revealed DL-Proline,5 Oxo  as the major compound. Having both antifungal and antibacterial activity.</jats:p

Assessing Organic Farming Adoption in Selected Districts of Tamil Nadu: Challenges, Practices, and Pathways for Growth

This study investigates the specific challenges and opportunities faced by organic farmers in Tamil Nadu, focusing on how region-specific practices influence the adoption and success of organic farming. It draws insights from a survey of 300 organic growers across five districts: Coimbatore, Dindigul, Erode, Tirupur, and Villupuram. This study is particularly relevant because there is a lack of comprehensive research on the specific challenges and opportunities faced by organic farmers in Tamil Nadu, with limited data on the long-term impact of government initiatives and region-specific management options, especially for smallholder farmers. Additionally, gaps exist in understanding the economic viability, market demand, and farmer perceptions of organic farming together with challenges like knowledge gaps, pest control, managing risks, and lower initial yields during the transition from conventional farming to organic farming. The research evaluated the factors affecting organic farming, including crop yield, the use of different organic formulations, the role of different information services, opportunities and challenges, and the impact on the perceived effectiveness of various agricultural outcomes. Various statistical analyses were performed, including K-means clustering, Poisson regression, and chi-square test. The results revealed significant variations in the adoption of various organic inputs and regionally practiced organic formulations. Crop residues (82.67%), Jeevamrith (78.33%), Amudhakaraisal (77.00%), and 3G-Extracts (74.00%) have high adoption rates, reflecting their perceived effectiveness and accessibility among farmers. However, the adoption of microbial bio-inputs such as Trichoderma viride (1.00%), Acetobacter spp. (19.00%), Azophos (consortium of phosphorus fixing microbes) (26.33%), and Azospirillum spp. (28.67%) was notably poor despite their known benefits for soil health and crop yield. The chi-square test also highlighted key challenges faced by farmers in adopting organic farming practices. The Poisson regression analysis showed significant positive impacts on crop growth and yield, disease resistance, insect control, overall plant health, and input efficiency due to organic farming. The study&rsquo;s insights are essential for developing targeted strategies to overcome barriers, supporting the growth and sustainability of organic farming. Addressing these challenges and promoting effective information channels will better equip stakeholders to support organic growers, contributing to more resilient and productive agricultural systems in the region