ANTIOXIDANT, ANTIHYPERGLYCEMIC AND ANTIGLYCATION PROPERTIES OF SOME SWERTIA SPECIES FROM WESTERN GHATS

Objective: Oxidative stress and Advanced Glycation End-products have been associated with diabetic complications. Therefore, natural compounds or extracts that possess both antioxidant and anti-glycation activities might have great therapeutic potential for treating diabetic complications. Methods: The main purpose of this study was to evaluate the total phenolics, total flavonoids, antioxidant, antihyperglycemic and anti-glycation properties of aqueous extracts of some Swertia species from Western Ghats. Results: The present study revealed that the S. Minor showed the highest amount of total phenolics, total flavonoids and antioxidant activities as compared to other species under study. Swertia angustifolia var. pulchella showed prominent decrease in blood glucose level and the S. lawii distillate showed the highest reduction in fructosamine content with greater anti-glycation property. Conclusion: All the Swertia species distillate analyzed in this study has exhibited potent hypoglycemic activity. Our study tends to support the traditional use of these medicinally important species and alternative source of diabetic medicines. Further study required to analyze the phytochemicals and their mechanism related to antioxidant, antidiabetic and antiglycation properties

Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.) – An important underutilized plant

The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of Salacia chinensis L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography- diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1- diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of S. chinensis and confirmed its medicinal and nutritional implications

Total phenolics, flavonoids and antioxidant properties of three Ceropegia species from Western Ghats of India

AbstractThe aim of present work was to assess the total phenolic content (TPC), total flavonoid content (TFC), phenolic compounds and antioxidant properties of various extracts of three Ceropegia spp.: Ceropegia spiralis, Ceropegia panchganiensis and Ceropegia evansii from Western Ghats of India. TPC of the samples varied from 0.3±0.2 to 28.5±0.3mg TAE/g FW, whereas, TFC of the samples ranged between 0.1±0.1 and 15.3±0.3mg RE/g FW. The major phenolic compounds identified were gallic acid, vanillin, cathechol and ferulic acid. All the extracts possess 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) as well as metal chelating ability and this was also supported by significant correlation with TPC and TFC. To the best of our knowledge, this is the first paper presenting comprehensive data on TPC, TFC, phenolic profile and antioxidant properties of the Ceropegia spp