Enzyme inactivation by photoexcited titanium dioxide (TiO₂) and prevention by encapsulation

Titanium dioxide (TiO 2 ) is used in sunscreens because it reflects and scatters UV light. However, TiO 2 can catalyze in vitro oxidative damage to DNA, to cultured human fibroblasts and can also inactivate the enzyme horseradish peroxidase (HRP) upon photoexcitation by UV light. The effects of photoexcited TiO 2 on two key enzymes involved in the skin's enzymatic antioxidant defense system, copper-zinc superoxide dismutase (CuZnSOD) and catalase, are reported here. Both enzymes are rapidly inactivated upon exposure to photoexcited TiO 2 , and their inactivation mechanisms are described. Hydroxyl radicals (OH · ), generated upon photoexcitation of TiO 2 , attack the active sites of the enzymes, leading to their inactivation. Copper release from CuZnSOD and heme destruction in catalase were observed, which explain the loss of activity. Encapsulation of TiO 2 in faujasite and mordenite, two different types of zeolites, greatly reduced the inactivation of CuZnSOD and catalase upon exposure to UVA light. These results suggest that encapsulation may lead to safer sunscreens based on supramolecular chemistry

Morphology and dynamics of inflated subaqueous basaltic lava flows

International audienceDuring eruptions onto low slopes, basaltic Pahoehoe lava can form thin lobes that progressively coalesce and inflate to many times their original thickness, due to a steady injection of magma beneath brittle and viscoelastic layers of cooled lava that develop sufficient strength to retain the flow. Inflated lava flows forming tumuli and pressure ridges have been reported in different kinds of environments, such as at contemporary subaerial Hawaiian-type volcanoes in Hawaii, La Réunion and Iceland, in continental environments (states of Oregon, Idaho, Washington), and in the deep sea at Juan de Fuca Ridge, the Galapagos spreading center, and at the East Pacific Rise (this study). These lava have all undergone inflation processes, yet they display highly contrasting morphologies that correlate with their depositional environment, the most striking difference being the presence of water. Lava that have inflated in subaerial environments display inflation structures with morphologies that significantly differ from subaqueous lava emplaced in the deep sea, lakes, and rivers. Their height is 2-3 times smaller and their length being 10-15 times shorter. Based on heat diffusion equation, we demonstrate that more efficient cooling of a lava flow in water leads to the rapid development of thicker (by 25%) cooled layer at the flow surface, which has greater yield strength to counteract its internal hydrostatic pressure than in subaerial environments, thus limiting lava breakouts to form new lobes, hence promoting inflation. Buoyancy also increases the ability of a lava to inflate by 60%. Together, these differences can account for the observed variations in the thickness and extent of subaerial and subaqueous inflated lava flows

Tectonic inheritance and continental rift architecture: Numerical and analogue models of the East African Rift System.

The western branch of the East African Rift is composed of an arcuate succession of elongate asymmetric basins, which differ in terms of interaction geometry, fault architecture and kinematics, and patterns of uplift/subsidence and erosion/sedimentation. The basins are located within Proterozoic mobile belts at the edge of the strong Tanzanian craton; surface geology suggests that the geometry of these weak zones is an important parameter in controlling rift development and architecture, although other processes have been proposed. In this study, we use lithosphere-scale numerical models and crustal-scale analogue experiments to shed light on the relations between preexisting structures and rift architecture. Results illustrate that on a regional scale, rift localization within the mobile belts at the curved craton's western border results in an arcuate rift system, which implies that under a constant extensional stress field, part of the western branch experienced orthogonal extension and part oblique extension. Largest depocenters are predicted to form mostly orthogonal to the extension direction, and smaller depocenters will form along the oblique parts of the rift. The varying extension direction along the rift zone furthermore results in lengthwise varying rift asymmetry, segmentation characteristics, and border fault architecture (trend, length, and kinematics). Analogue models predict that discrete upper crustal fabrics may influence the location of accommodation zones and control the architecture of extension-related faults at a local scale. Models support that fabric reactivation is responsible for the oblique-slip kinematics on faults and for the development of Z-shaped or arcuate normal faults typically documented in nature. Copyright 2007 by the American Geophysical Union

Autonomous quantum error correction of Gottesman-Kitaev-Preskill states

The Gottesman-Kitaev-Preskill (GKP) code encodes a logical qubit into a bosonic system with resilience against single-photon loss, the predominant error in most bosonic systems. Here we present experimental results demonstrating quantum error correction of GKP states based on reservoir engineering of a superconducting device. Error correction is made autonomous through an unconditional reset of an auxiliary transmon qubit. The lifetime of the logical qubit is shown to be increased from quantum error correction, therefore reaching the point at which more errors are corrected than generated.Comment: 6 pages, 3 figures + 26 pages, 12 figure

Сверхлегкие генераторные модули для КВЧ-терапии

Разработаны миниатюрные генераторные модули для КВЧ-терапии, лег-ко фиксируемые в любом месте тела пациента. Могут быть использованы не только в медицине

Mechanism of 53BP1 activity regulation by RNA-binding TIRR and a designer protein

Dynamic protein interaction networks such as DNA double-strand break (DSB) signaling are modulated by post-translational modifications. The DNA repair factor 53BP1 is a rare example of a protein whose post-translational modification-binding function can be switched on and off. 53BP1 is recruited to DSBs by recognizing histone lysine methylation within chromatin, an activity directly inhibited by the 53BP1-binding protein TIRR. X-ray crystal structures of TIRR and a designer protein bound to 53BP1 now reveal a unique regulatory mechanism in which an intricate binding area centered on an essential TIRR arginine residue blocks the methylated-chromatin-binding surface of 53BP1. A 53BP1 separation-of-function mutation that abolishes TIRR-mediated regulation in cells renders 53BP1 hyperactive in response to DSBs, highlighting the key inhibitory function of TIRR. This 53BP1 inhibition is relieved by TIRR-interacting RNA molecules, providing proof-of-principle of RNA-triggered 53BP1 recruitment to DSBs

Efficient Production of HIV-1 Virus-Like Particles from a Mammalian Expression Vector Requires the N-Terminal Capsid Domain

It is now well accepted that the structural protein Pr55Gag is sufficient by itself to produce HIV-1 virus-like particles (VLPs). This polyprotein precursor contains different domains including matrix, capsid, SP1, nucleocapsid, SP2 and p6. In the present study, we wanted to determine by mutagenesis which region(s) is essential to the production of VLPs when Pr55Gag is inserted in a mammalian expression vector, which allows studying the protein of interest in the absence of other viral proteins. To do so, we first studied a minimal Pr55Gag sequence called Gag min that was used previously. We found that Gag min fails to produce VLPs when expressed in an expression vector instead of within a molecular clone. This failure occurs early in the cell at the assembly of viral proteins. We then generated a series of deletion and substitution mutants, and examined their ability to produce VLPs by combining biochemical and microscopic approaches. We demonstrate that the matrix region is not necessary, but that the efficiency of VLP production depends strongly on the presence of its basic region. Moreover, the presence of the N-terminal domain of capsid is required for VLP production when Gag is expressed alone. These findings, combined with previous observations indicating that HIV-1 Pr55Gag-derived VLPs act as potent stimulators of innate and acquired immunity, make the use of this strategy worth considering for vaccine development

Numerical modelling study on the flexural uplift of the Transantarctic Mountains

In this study, based on a 2-D thermomechanical finite element model, the uplift of the Transantarctic Mountains (TAM) is discussed in relation to the flexural uplift of a rheologically layered lithosphere, which is described by Vening-Meinesz's cantilever kinematics. The general model behaviour shows that the thickness of the crust and the geothermal gradient in the lithosphere are the principal factors in controlling the effective elastic thickness (

In situ estimation of ice crystal properties at the South Pole using LED calibration data from the IceCube Neutrino Observatory

The IceCube Neutrino Observatory instruments about 1 km3 of deep, glacial ice at the geographic South Pole. It uses 5160 photomultipliers to detect Cherenkov light emitted by charged relativistic particles. An unexpected light propagation effect observed by the experiment is an anisotropic attenuation, which is aligned with the local flow direction of the ice. We examine birefringent light propaga- tion through the polycrystalline ice microstructure as a pos- sible explanation for this effect. The predictions of a first- principles model developed for this purpose, in particular curved light trajectories resulting from asymmetric diffusion, provide a qualitatively good match to the main features of the data. This in turn allows us to deduce ice crystal properties. Since the wavelength of the detected light is short compared to the crystal size, these crystal properties include not only the crystal orientation fabric, but also the average crystal size and shape, as a function of depth. By adding small empiri- cal corrections to this first-principles model, a quantitatively accurate description of the optical properties of the IceCube glacial ice is obtained. In this paper, we present the exper- imental signature of ice optical anisotropy observed in Ice- Cube light-emitting diode (LED) calibration data, the theory and parameterization of the birefringence effect, the fitting procedures of these parameterizations to experimental data, and the inferred crystal propertie