Thermonuclear explosions of rapidly rotating white dwarfs - II. Detonations

Context: Superluminous type Ia supernovae (SNe Ia) may be explained by super-Chandrasekhar-mass explosions of rapidly rotating white dwarfs (WDs). In a preceding paper, we showed that the deflagration scenario applied to rapidly rotating WDs generates explosions that cannot explain the majority of SNe Ia. Aims: Rotation of the progenitor star allows super-Chandrasekhar-mass WDs to form that have a shallower density stratification. We use simple estimates of the production of intermediate and iron group elements in pure detonations of rapidly rotating WDs to assess their viability in explaining rare SNe Ia. Methods: We numerically construct WDs in hydrostatic equilibrium that rotate according to a variety of rotation laws. The explosion products are estimated by considering the density stratification and by evaluating the result of hydrodynamics simulations. Results: We show that a significant amount of intermediate mass elements is produced for theoretically motivated rotation laws, even for prompt detonations of WDs. Conclusions: Rapidly rotating WDs that detonate may provide an explanation of rare superluminous SNe Ia in terms of both burning species and explosion kinematics.Comment: 7 pages, 5 figures, accepted for publication by A&

Thermonuclear explosions of rapidly rotating white dwarfs - I. Deflagrations

Context: Turbulent deflagrations of Chandrasekhar mass White Dwarfs are commonly used to model Type Ia Supernova explosions. In this context, rapid rotation of the progenitor star is plausible but has so far been neglected. Aims: The aim of this work is to explore the influence of rapid rotation on the deflagration scenario. Methods: We use three dimensional hydrodynamical simulations to model turbulent deflagrations ignited within a variety of rapidly rotating CO WDs obeying rotation laws suggested by accretion studies. Results: We find that rotation has a significant impact on the explosion. The flame develops a strong anisotropy with a preferred direction towards the stellar poles, leaving great amounts of unburnt matter along the equatorial plane. Conclusions: The large amount of unburnt matter is contrary to observed spectral features of SNe Ia. Thus, rapid rotation of the progenitor star and the deflagration scenario are incompatible in order to explain SNe Ia.Comment: 13 pages, 10 figures, accepted for publication by A&

Tunneling Rate for Superparamagnetic Particles by the Instanton Method

We derive the tunneling rate for paramagnetic molecules in the context of a collective spin model. By means of path integral methods an analytical expression is derived. Given the very large spins in question (s ~ 3000 hbar), the observation of magnetization changes due to pure unitary tunnel effects is unlikely.Comment: 16 pages, 2 figure

Adaptive microfluidic gradient generator for quantitative chemotaxis experiments

Chemotactic motion in a chemical gradient is an essential cellular function that controls many processes in the living world. For a better understanding and more detailed modelling of the underlying mechanisms of chemotaxis, quantitative investigations in controlled environments are needed. We developed a setup that allows us to separately address the dependencies of the chemotactic motion on the average background concentration and on the gradient steepness of the chemoattractant. In particular, both the background concentration and the gradient steepness can be kept constant at the position of the cell while it moves along in the gradient direction. This is achieved by generating a well-defined chemoattractant gradient using flow photolysis. In this approach, the chemoattractant is released by a light-induced reaction from a caged precursor in a microfluidic flow chamber upstream of the cell. The flow photolysis approach is combined with an automated real-time cell tracker that determines changes in the cell position and triggers movement of the microscope stage such that the cell motion is compensated and the cell remains at the same position in the gradient profile. The gradient profile can be either determined experimentally using a caged fluorescent dye or may be alternatively determined by numerical solutions of the corresponding physical model. To demonstrate the function of this adaptive microfluidic gradient generator, we compare the chemotactic motion of Dictyostelium discoideum cells in a static gradient and in a gradient that adapts to the position of the moving cell

The Reduced Form of a Block Recursive Model

Various estimators of the reduced form of a block recursive model are investigated and compared to each other. In particular it is shown that the structural reduced form estimator, which results from estimating separately each block of the block recursive model by some efficient method and then solving the system for the endogenous variables, is more efficient than the OLS estimator of the reduced form. Other reduced form estimators derived from OLS or Two Stage LS estimators of a partially reduced form have intermediate efficiency properties. The paper has been published in Schneeweiss et al (2001), but without the appendices

Physiological and Molecular Effects of in vivo and ex vivo Mild Skin Barrier Disruption

The success of topically applied treatments on skin relies on the efficacy of skin penetration. In order to increase particle or product penetration, mild skin barrier disruption methods can be used. We previously described cyanoacrylate skin surface stripping as an efficient method to open hair follicles, enhance particle penetration, and activate Langerhans cells. We conducted ex vivo and in vivo measurements on human skin to characterize the biological effect and quantify barrier disruption-related inflammation on a molecular level. Despite the known immunostimulatory effects, this barrier disruption and hair follicle opening method was well accepted and did not result in lasting changes of skin physiological parameters, cytokine production, or clinical side effects. Only in ex vivo human skin did we find a discrete increase in IP-10, TGF-β, IL-8, and GM-CSF mRNA. The data underline the safety profile of this method and demonstrate that the procedure per se does not cause substantial inflammation or skin damage, which is also of interest when applied to non-invasive sampling of biomarkers in clinical trials

A Guide for Outcomes Evaluation and Sustainability of a Dedicated Education Unit within an Academic-Practice Partnership

The Dedicated Education Unit (DEU) has emerged as an innovative approach to providing a positive, high-quality, collaborative clinical learning environment that fosters the growth and learning of undergraduate nursing students. Additionally, the DEU model has demonstrated success in beginning to bridge the education-practice gap, tackling the faculty shortage, and easing the new graduate transition from education to practice; however, developing, evaluating and sustaining an economically successful DEU takes thoughtful, strategic planning. The challenging nature of developing and sustaining a DEU that mutually benefits both the academic and clinical partner over time, reveals the need for more guidance to secure long-term benefits of maintaining the DEU within an academic-practice partnership. While the literature is robust with current knowledge on the positive practicality of the DEU, there are few data available regarding expected outcomes and long-term planning for sustainability for a successful DEU within an academic-practice partnership. The purpose of this Doctor of Nursing Practice (DNP) project was to develop a guide inclusive of strategies for evaluating objective, subjective, and economic outcomes, and the long-term sustainability of a DEU within an academic-practice partnership. The literature was extensively reviewed to find evidence gaps and areas needing improvement in existing DEU models. Subsequently, a guide was developed detailing strategies for implementing and evaluating objective, subjective, and economic outcomes that benefit both collaborators of the academic-practice partnership. The guide includes measurement tools to evaluate student and nurse satisfaction, in addition to the evaluation of the clinical learning environment and economic benefits of nurse retention, decreased orientation and training times, and decreased recruitment efforts. The guide also includes multiple resources for the implementation and sustainability of new and existing DEUs. The development and implementation of this DNP project will allow the leadership team of the academic-practice partnership to measure short- and long-term outcomes and further demonstrate the DEU’s benefits to the non-medical leadership at the medical center and the University. The guide translates and expands the available evidence to create a manual for evaluation and sustainability, inclusive of several psychometrically tested tools and recommendations for the partnership leaders to consider when evaluating outcomes and sustainability of the DEU. The guide will also serve as an exemplar for others considering implementing and maintaining a DEU within their institutions

Spectral modelling of the "Super-Chandra" Type Ia SN 2009dc - testing a 2 M_sun white dwarf explosion model and alternatives

Extremely luminous, super-Chandrasekhar (SC) Type Ia Supernovae (SNe Ia) are as yet an unexplained phenomenon. We analyse a well-observed SN of this class, SN 2009dc, by modelling its photospheric spectra with a spectral synthesis code, using the technique of 'Abundance Tomography'. We present spectral models based on different density profiles, corresponding to different explosion scenarios, and discuss their consistency. First, we use a density structure of a simulated explosion of a 2 M_sun rotating C-O white dwarf (WD), which is often proposed as a possibility to explain SC SNe Ia. Then, we test a density profile empirically inferred from the evolution of line velocities (blueshifts). This model may be interpreted as a core-collapse SN with an ejecta mass ~ 3 M_sun. Finally, we calculate spectra assuming an interaction scenario. In such a scenario, SN 2009dc would be a standard WD explosion with a normal intrinsic luminosity, and this luminosity would be augmented by interaction of the ejecta with a H-/He-poor circumstellar medium. We find that no model tested easily explains SN 2009dc. With the 2 M_sun WD model, our abundance analysis predicts small amounts of burning products in the intermediate-/high-velocity ejecta (v > 9000 km/s). However, in the original explosion simulations, where the nuclear energy release per unit mass is large, burned material is present at high v. This contradiction can only be resolved if asymmetries strongly affect the radiative transfer or if C-O WDs with masses significantly above 2 M_sun exist. In a core-collapse scenario, low velocities of Fe-group elements are expected, but the abundance stratification in SN 2009dc seems 'SN Ia-like'. The interaction-based model looks promising, and we have some speculations on possible progenitor configurations. However, radiation-hydro simulations will be needed to judge whether this scenario is realistic at all.Comment: 22 pages, 12 figures, published in MNRAS. V2: several small corrections (typos, style

Characterization of the symbiotic bacterial partners in phototrophic consortia

Bacterial interactions play a major role in nature, but are poorly understood, because of the lack of adequate model systems. Phototrophic consortia represent the most highly developed type of interspecific bacterial association due to the precise spatial arrangement of phototrophic green sulfur bacteria (GSB) around a heterotrophic central bacterium. Therefore, they are valuable model systems for the study of symbiosis, signal transduction, and coevolution between different bacteria. This thesis summarizes a series of laboratory experiments with the objective of elucidating the molecular, physiological and phylogenetical properties of the two bacterial partners in the symbiotic phototrophic consortium "Chlorochromatium aggregatum". The central bacterium of “C. aggregatum” had been identified as a Betaproteobacterium, however, it could not be characterized further due to the low amount of consortia in enrichment cultures. In this work a suitable method for enrichment and isolation of DNA of the central bacterium of "C. aggregatum" has been established using cesium chloride-bisbenzimidazole equilibrium density gradient centrifugation (Chapter 3). In density gradients, genomic DNA of the central bacterium of “C. aggregatum” formed a distinct band, which could be detected by real-time PCR. Using this method, the GC-content of the central bacterium was estimated to be 55.6%. Furthermore, its precise phylogenetic position was determined and it was shown to represent a novel and phylogenetically isolated lineage of the Comamonadaceae within the -subgroup of the Proteobacteria. Chapter 4 describes the detection of a new, highly diverse subcluster of Betaproteobacteria, which contains several central bacteria of phototrophic consortia. Genomic DNA of the central bacterium of “C. aggregatum” was enriched several hundred fold by employing a selective method for growth of consortia in a monolayer biofilm followed by a purification of the central bacterial genome by density gradient centrifugation. A combination of molecular methods revealed that two rrn operons of the central bacterium are arranged in a tandem fashion. This rare gene order was exploited to screen various natural microbial communities. A diverse and previously unknown subgroup of Betaproteobacteria was discovered in the chemocline of Lake Dagow, Eastern Germany. All 16S rRNA gene sequences recovered are related to that of the central bacterium of “C. aggregatum”. Phylogenetic analyses showed, that the central, chemotrophic symbionts of phototrophic consortia have a polyphyletic origin, just like their phototrophic counterparts. This indictates that not only different GSB but also different Betaproteobacteria have adapted to life in this type of symbiosis. Chapter 5 focuses on the isolation of the epibiont of “C. aggregatum” from a consortia enrichment culture and its description as Chlorobium chlorochromatii strain CaD. It represents a novel species within the genus Chlorobium and is characterized by physiological properties typical for GSB. However, the symbiotic strain differs from free-living GSB in the distribution of its chlorosomes and the presence of a conspicuous additional structure at the attachment-site to the central bacterium. Its capability to grow in pure culture indicates that it is not obligately symbiotic. The natural habitat of GSB and phototrophic consortia is the chemocline of stratified lakes. Therefore, the physiological response to oxygen exposure of the epibiont and the free-living GSB Chlorobium limicola has been investigated (Chapter 6). It was shown that GSB are able to survive oxygen exposure and have developed several strategies for oxygen detoxification. Genome annotation revealed the presence of several enzymes involved in oxygen detoxification in all currently sequenced GSB genomes. Phylogenetic analyses showed that most of these enzymes likely were present in the common ancestor of this group. The activity of some of those enzymes could be confirmed. Since carotenoids also act as antioxidants, the carotenoid composition of the epibiont was investigated. In contrast to all other GSB it lacks chlorobactene, the major carotenoid in green-coloured GSB. In addition, 7,8-dihydro--carotene has been identified in the epibiont as a novel carotenoid in nature. Substantial progress has been made in the course of this study not only with the establishment of a method facilitating genome sequencing of the central bacterium of “C. aggregatum”, but also with the developement of a molecular screening tool for central bacteria of phototrophic consortia. The resulting sequences will enable the direct comparison of the phylogeny of both bacterial partners in different phototrophic consortia and hence will provide the unique opportunity to assess for the first time the process of the coevolution of a bacteria-bacteria-symbiosis