Protein Kinase C θ Affects Ca2+ Mobilization and NFAT Activation in Primary Mouse T Cells

Protein kinase C (PKC)θ is an established component of the immunological synapse and has been implicated in the control of AP-1 and NF-κB. To study the physiological function of PKCθ, we used gene targeting to generate a PKCθ null allele in mice. Consistently, interleukin 2 production and T cell proliferative responses were strongly reduced in PKCθ-deficient T cells. Surprisingly, however, we demonstrate that after CD3/CD28 engagement, deficiency of PKCθ primarily abrogates NFAT transactivation. In contrast, NF-κB activation was only partially reduced. This NFAT transactivation defect appears to be secondary to reduced inositol 1,4,5-trisphosphate generation and intracellular Ca2+ mobilization. Our finding suggests that PKCθ plays a critical and nonredundant role in T cell receptor–induced NFAT activation

A blockchain-based framework for trusted quality data sharing towards zero-defect manufacturing

There is a current wave of a new generation of digital solutions based on intelligent systems, hybrid digital twins and AI-driven optimization tools to assure quality in smart factories. Such digital solutions heavily depend on quality-related information within the supply chain business ecosystem to drive zero-waste value chains. To empower zero-waste value chain strategies with meaningful, reliable, and trustful data, there must be a solution for end-to-end industrial data traceability, trust, and security across multiple process chains or even inter-organizational supply chains. In this paper, we first present Product, Process, and Data quality services to drive zero-waste value chain strategies. Following this, we present the Trusted Framework (TF), which is a key enabler for the secure and effective sharing of quality-related information within the supply chain business ecosystem, and thus for quality optimization actions towards zero-defect manufacturing. The TF specification includes the data model and format of the Process/Product/Data (PPD) Quality Hallmark, the OpenAPI exposed to factory system and a comprehensive Identity Management layer, for secure horizontal- and vertical quality data integration. The PPD hallmark and the TF already address some of the industrial needs to have a trusted approach to share quality data between the different stakeholders of the production chain to empower zero-waste value chain strategies.publishedVersio