Substrate specificity provides insights into the sugar donor recognition mechanism of O-GlcNAc transferase (OGT).

O-Linked β-N-acetylglucosaminyl transferase (OGT) plays an important role in the glycosylation of proteins, which is involved in various cellular events. In human, three isoforms of OGT (short OGT [sOGT]; mitochondrial OGT [mOGT]; and nucleocytoplasmic OGT [ncOGT]) share the same catalytic domain, implying that they might adopt a similar catalytic mechanism, including sugar donor recognition. In this work, the sugar-nucleotide tolerance of sOGT was investigated. Among a series of uridine 5'-diphosphate-N-acetylglucosamine (UDP-GlcNAc) analogs tested using the casein kinase II (CKII) peptide as the sugar acceptor, four compounds could be used by sOGT, including UDP-6-deoxy-GlcNAc, UDP-GlcNPr, UDP-6-deoxy-GalNAc and UDP-4-deoxy-GlcNAc. Determined values of Km showed that the substitution of the N-acyl group, deoxy modification of C6/C4-OH or epimerization of C4-OH of the GlcNAc in UDP-GlcNAc decreased its affinity to sOGT. A molecular docking study combined with site-directed mutagenesis indicated that the backbone carbonyl oxygen of Leu653 and the hydroxyl group of Thr560 in sOGT contributed to the recognition of the sugar moiety via hydrogen bonds. The close vicinity between Met501 and the N-acyl group of GlcNPr, as well as the hydrophobic environment near Met501, were responsible for the selective binding of UDP-GlcNPr. These findings illustrate the interaction of OGT and sugar nucleotide donor, providing insights into the OGT catalytic mechanism

Tackling unemployment in China: state capacity and governance issues

This paper considers China's state capacity and changing governance as revealed through its policies to tackle unemployment. Despite high levels of growth, economic restructuring has resulted in rising unemployment over the last decade. The Chinese state has been able to manage job losses from state enterprises, demonstrating some state capacity in relation to this sector and some persistent command economy governance mechanisms. However both design and implementation of policies to compensate and assist particular groups among the unemployed have been shaped by weak state capacity in several other areas. First, capacity to gather accurate employment data is limited, meaning local and central governments do not have a good understanding of the extent and nature of unemployment. Second, the sustainability of supposedly mandatory unemployment insurance schemes is threatened by poor capacity to enforce participation. Third, poor central state capacity to ensure local governments implement policies effectively leads to poor unemployment insurance fund capacity, resulting in provision for only a narrow segment of the unemployed and low quality employment services. Although the adoption of unemployment insurance (and its extension to employers and employees in the private sector), the introduction of a Labour Contract Law in 2007, and the delivery of employment services by private businesses indicate a shift towards the use of new governance mechanisms based on entitlement, contract and private sector delivery of public-sector goods, that shift is undermined by poor state capacity in relation to some of these new mechanisms

Strong ionospheric field‐aligned currents for radial interplanetary magnetic fields

The present work has investigated the configuration of field‐aligned currents (FACs) during a long period of radial interplanetary magnetic field (IMF) on 19 May 2002 by using high‐resolution and precise vector magnetic field measurements of CHAMP satellite. During the interest period IMF B y and B z are weakly positive and B x keeps pointing to the Earth for almost 10 h. The geomagnetic indices D s t is about −40 nT and AE about 100 nT on average. The cross polar cap potential calculated from Assimilative Mapping of Ionospheric Electrodynamics and derived from DMSP observations have average values of 10–20 kV. Obvious hemispheric differences are shown in the configurations of FACs on the dayside and nightside. At the south pole FACs diminish in intensity to magnitudes of about 0.1 μA/m 2 , the plasma convection maintains two‐cell flow pattern, and the thermospheric density is quite low. However, there are obvious activities in the northern cusp region. One pair of FACs with a downward leg toward the pole and upward leg on the equatorward side emerge in the northern cusp region, exhibiting opposite polarity to FACs typical for duskward IMF orientation. An obvious sunward plasma flow channel persists during the whole period. These ionospheric features might be manifestations of an efficient magnetic reconnection process occurring in the northern magnetospheric flanks at high latitude. The enhanced ionospheric current systems might deposit large amount of Joule heating into the thermosphere. The air densities in the cusp region get enhanced and subsequently propagate equatorward on the dayside. Although geomagnetic indices during the radial IMF indicate low‐level activity, the present study demonstrates that there are prevailing energy inputs from the magnetosphere to both the ionosphere and thermosphere in the northern polar cusp region. Key Points A pair of strong FACs emerges with opposite polarity to DPY FACs Obvious sunward plasma flow channel persists during the period Enhanced air densities are found in the cusp regionPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/107563/1/jgra51028.pd

Nonuniform symmetry breaking in noncommutative λΦ4\lambda \Phi^4 theory

The spontaneous symmetry breaking in noncommutative $\lambda\Phi^4$ theory has been analyzed by using the formalism of the effective action for composite operators in the Hartree-Fock approximation. It turns out that there is no phase transition to a constant vacuum expectation of the field and the broken phase corresponds to a nonuniform background. By considering $=A \cos(\vec Q \cdot \vec x)$ the generated mass gap depends on the angles among the momenta $\vec k$ and $\vec Q$ and the noncommutativity parameter $\vec\theta$. The order of the transition is not easily determinable in our approximation.Comment: 18 pages, 4 figures, added reference

Resumming the large-N approximation for time evolving quantum systems

In this paper we discuss two methods of resumming the leading and next to leading order in 1/N diagrams for the quartic O(N) model. These two approaches have the property that they preserve both boundedness and positivity for expectation values of operators in our numerical simulations. These approximations can be understood either in terms of a truncation to the infinitely coupled Schwinger-Dyson hierarchy of equations, or by choosing a particular two-particle irreducible vacuum energy graph in the effective action of the Cornwall-Jackiw-Tomboulis formalism. We confine our discussion to the case of quantum mechanics where the Lagrangian is $L(x,\dot{x}) = (1/2) \sum_{i=1}^{N} \dot{x}_i^2 - (g/8N) [ \sum_{i=1}^{N} x_i^2 - r_0^2 ]^{2}$. The key to these approximations is to treat both the $x$ propagator and the $x^2$ propagator on similar footing which leads to a theory whose graphs have the same topology as QED with the $x^2$ propagator playing the role of the photon. The bare vertex approximation is obtained by replacing the exact vertex function by the bare one in the exact Schwinger-Dyson equations for the one and two point functions. The second approximation, which we call the dynamic Debye screening approximation, makes the further approximation of replacing the exact $x^2$ propagator by its value at leading order in the 1/N expansion. These two approximations are compared with exact numerical simulations for the quantum roll problem. The bare vertex approximation captures the physics at large and modest $N$ better than the dynamic Debye screening approximation.Comment: 30 pages, 12 figures. The color version of a few figures are separately liste

Quantifying the pathway and predicting spontaneous emulsification during material exchange in a two phase liquid system

Kinetic restriction of a thermodynamically favourable equilibrium is a common theme in materials processing. The interfacial instability in systems where rate of material exchange is far greater than the mass transfer through respective bulk phases is of specific interest when tracking the transient interfacial area, a parameter integral to short processing times for productivity streamlining in all manufacturing where interfacial reaction occurs. This is even more pertinent in high-temperature systems for energy and cost savings. Here the quantified physical pathway of interfacial area change due to material exchange in liquid metal-molten oxide systems is presented. In addition the predicted growth regime and emulsification behaviour in relation to interfacial tension as modelled using phase-field methodology is shown. The observed in-situ emulsification behaviour links quantitatively the geometry of perturbations as a validation method for the development of simulating the phenomena. Thus a method is presented to both predict and engineer the formation of micro emulsions to a desired specification

Explaining Physicians’ Acceptance and Resistance to the NHI Pharmacloud: A Theoretical Model and Empirical Test

The PharmaCloud allows physicians to streamline many of their healthcare processes and ensure patient safety in a more efficient and cost-effective manner. Despite its great potential, however, there are gaps in our understanding of how physicians evaluate change in relation to the PharmaCloud and why they decide to resist it. Thus, this study develops an integrated model to explain physicians’ intention to use the PharmaCloud and their intention to resist it. A field survey was conducted in Taiwan to collect data from physicians. Structural equation modeling (SEM) using the partial least squares (PLS) method was employed to test the research model. The results show that physicians’ resistance to the use of the PharmaCloud is the result of regret avoidance, inertia, perceived value, transition costs, and perceived threat. Information quality, system quality, and service quality are shown to have positive and direct effects on physicians’ intention to use the PharmaCloud. Our study illustrates the importance of incorporating user resistance in technology acceptance studies in general and health technology usage studies in particular, providing grounds for a model of resistance that can serve as the starting point for future research in this relatively unexplored yet potentially fertile area of research