Effects of dietary crude protein and calcium/ phosphorus content on growth, nitrogen and mineral retention in broiler chickens

This experiment was conducted to examine the effect of three levels of crude protein (CP) (NRC, 15% more than NRC and 15% less than NRC) and three levels of Ca and available P (Av. P) (NRC, 15% more than NRC and 15% less than NRC) on performance of broilers from hatching until 21 days of age. The experimental design was a completely randomized design (CRD), with a 3 × 3 factorial arrangement of nine dietary treatments. Each treatment combination had four replicate pens (10 birds per pen). Results of this experiment showed that CP content had no significant effect on feed and water intake. However, body weight gain (BWG) significantly reduced and FCR increased (P < 0.05) by 15% change in CP content of diet. Fifteen percent increase in mineral content of diets had no significant effect on feed intake. However, increase in Ca and Av. P significantly increased BWG and resulted in an improved feed conversion ratio (FCR) and increased water intake. There was an interactive effect of CP by Ca and Av. P levels on feed intake, BWG and FCR. A change in CP or mineral content of the diets had no significant effect on blood parameters except for potassium concentration (K+). Reducing CP, Ca and Av. P content of the diet significantly (P < 0.001) increased P, Ca and N retention. Reduction in CP content of the diet led to a decrease in length, P and tibia bone ash. There was an interactive effect of CP by Ca and Av. P on the retention of Ca, N and ash of tibia bone.Key words: Crude protein, Ca and Av. P levels, broilers, N retention

Evaluation of corn meal on performance, carcass characteristics and nutrient digestibility of male broiler chickens

To study the effect of corn meal (CM) on performance, carcass characteristics and nutrient digestibility of chickens in a completely randomized design experiment with 4 × 2 factorial arrangement and 4 replicates per treatment, 384 Ross male broiler chickens in a 49 days period were evaluated. Treatments were 0 (CM0), 15 (CM15), 30 (CM30), or 45 (CM45) CM to replace corn and 2 levels of commercial NSP-degrading enzyme (0 and 0.025%). Corn meal had no effect (P > 0.05) on body weight gain, feed intake and carcass characteristics during the whole brooding period. Dietary corn meal level (CM45) had significant effect on digestibility of fat, apparent metabolizable energy (AME) and apparent metabolizable energy corrected for nitrogen (AMEn). However, enzyme supplementation significantly increased phosphorous digestibility (P < 0.05). Interaction between corn meal level and enzyme supplementation had significant effect on excreta dry matter (DM), organic matter (OM), crude protein (CP), AME and AMEn during 0 to 49 days of age. Together, the results suggested that replacement of corn by corn meal may affect the digestibility of OM, CP, AME, AMEn, and enzyme supplementation has some beneficial effects on theses traits.Keywords: Corn meal, enzyme, broiler chickens, performance, carcass characteristics, nutrient digestibilit

Efficacy of natural zeolite and pigments on yolk color and performance of laying hens

An in vivo study was conducted to evaluate pigment adsorptive ability of a natural zeolite in laying hens. This experiment was performed with three hundred sixty Hy-line W-36 strain of laying hens at 43 weeks of age. After a two weeks adaptation period, they received six experimental diets with a 3 × 2 factorial arrangement in a completely randomized design. The experimental groups were fed with cornsoy- wheat based diets containing three levels of natural zeolite (0.0, 1.5 and 3.0%) and 2 levels of synthetic pigment (0.0 and 0.04%) for a 6-week period. Each treatment contained 60 birds, which were randomly divided into 4 replicates of 15 birds each. The results showed that egg production, egg weight, shell thickness and the shell percent values did not significantly differ between treatments, but that the yolk color index was significantly reduced and increased by the addition of zeolite and pigment levels to the diet, respectively.Key words: Natural zeolite, pigment, yolk color, laying hens

The Anti-staphylococcus Aureus Effect Of Combined Echinophora Platyloba Essential Oil And Liquid Smoke In Beef

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke.551117124Student Research Committee, Shahid Beheshti University of Medical Sciences [1395-77359]CNPq-TWAS [324027 4290]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

The effect of acceptance and commitment therapy on quality of life in patients with orthopedic problems

Background: Bone and joint diseases have an effect on the clinical situation, high-quality of existence (QOL), and its dimensions of incapacity, causing supercharges for distinctive countries. This examine become aimed to assess the impact of acceptance and commitment therapy on QOL in orthopedic sufferers. Methods: This reading became a quasi-experimental performed as a parallel layout with pre-test and post-test. Patients stated the Promised Physiotherapy center in Mashhad decided on and had been randomly assigned to experimental (n = 12) and manipulate agencies (n = 12). The experimental organization acquired 8 weekly classes for three months, at the same time as the management organization did not receive any intervention. Statistics had been acquired by a standard QOL questionnaire and then records examine turned into finished the use of SPSS software version 25 and multivariate evaluation of covariance. Results: The average age inside the investigational agencies changed into 31.75 ± 4.71 years within the manage companies became 29.83 ± 5.27 years. Also, the common disorder timespan of the experimental organizations and manipulated businesses have been 5.64 ± 5.28 and 5.62 ± 5.81, correspondingly. The common post-test ratings of the experimental companies compared to the pre-test showed growth in subscales of QOL, however, the control agencies did no longer show such an alteration inside the post-test averages. (P-value = 0.0001) Conclusion: the use of acceptance and commitment remedy as an effective and complementary psychological intervention in enhancing the QOL in orthopedic sufferers is usually recommended

Utjecaj esencijalnog ulja biljke Echinophora platyloba i primarnog kondenzata dima na suzbijanje rasta bakterije Staphylococcus aureus u goveđem mesu

In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke.U radu je ispitan pojedinačni i zbirni učinak esencijalnog ulja biljke Echinophora platyloba i primarnog kondenzata dima na suzbijanje rasta bakterije Staphylococcus aureus u uzorcima goveđeg mesa. Pomoću automatskog sustava za detekciju mikrobiološkog rasta i turbidimetrije određene su minimalna inhibicijska koncentracija i minimalna baktericidna koncentracija esencijalnog ulja i primarnog kondenzata dima. Pojedinačni i zbirni antibakterijski učinak esencijalnog ulja i primarnog kondenzata dima ispitani su disk difuzijskom metodom, određivanjem generacijskog vremena i mjerenjem apsorbancije otpuštenog staničnog sadržaja bakterije Staphylococcus aureus. Osim toga, ispitana je interakcija između ta dva antimikrobna agensa mikrorazrjeđenjem u bujonu (tzv. checkerboard testom) i određivanjem indeksa interakcije. Minimalna inhibicijska koncentracija esencijalnog ulja bila je 7200 mg/L, a primarnog kondenzata dima 5500 mg/L, dok je minimalna baktericidna koncentracija esencijalnog ulja bila 8500 mg/L, a primarnog kondenzata dima 8000 mg/L. Ispitana su organoleptička svojstva uzoraka mesa i utvrđeno je da dodatak 0,05 g esencijalnog ulja i 0,6 g kondenzata dima u 100 g uzorka nije utjecao na prihvatljivost proizvoda. Esencijalno ulje biljke Echinophora platyloba imalo je slabiji učinak na suzbijanje rasta bakterije Staphylococcus aureus od kombinacije esencijalnog ulja i primarnog kondenzata dima

Postupci proizvodnje propionske kiseline, dosadašnje spoznaje i mogućnosti

During the past years, there has been a growing interest in the bioproduction of propionic acid by Propionibacterium. One of the major limitations of the existing models lies in their low productivity yield. Hence, many strategies have been proposed in order to circumvent this obstacle. This article provides a comprehensive synthesis and review of important biotechnological aspects of propionic acid production as a common ingredient in food and biotechnology industries. We first discuss some of the most important production processes, mainly focusing on biological production. Then, we provide a summary of important propionic acid producers, including Propionibacterium freudenreichii and Propionibacterium acidipropionici, as well as a wide range of reported growth/production media. Furthermore, we describe bioprocess variables that can have impact on the production yield. Finally, we propose methods for the extraction and analysis of propionic acid and put forward strategies for overcoming the limitations of competitive microbial production from the economical point of view. Several factors influence the propionic acid concentration and productivity such as culture conditions, type and bioreactor scale; however, the pH value and temperature are the most important ones. Given that there are many reports about propionic acid production from glucose, whey permeate, glycerol, lactic acid, hemicelluloses, hydrolyzed corn meal, lactose, sugarcane molasses and enzymatically hydrolyzed whole wheat flour, only few review articles evaluate biotechnological aspects, i.e. bioprocess variables.U posljednje je vrijeme porastao interes za biološku proizvodnju propionske kiseline s pomoću bakterije Propionibacterium. Glavni nedostatak postojećih modela je slab prinos, zbog čega su predložene različite strategije rješavanja tog problema. U ovom su radu opsežno prikazana bitna biotehnološka obilježja postupaka dobivanja propionske kiseline, jednog od uobičajenih sastojaka u prehrambenoj i biotehnološkoj proizvodnji. U prvom dijelu opisujemo najznačajnije proizvodne procese, s naglaskom na biološku proizvodnju, a zatim navodimo bakterije koje proizvode propionsku kiselinu, kao što su Propionibacterium freudenreichii i Propionibacterium acidipropionici, te različite podloge za rast ili proizvodnju. Osim toga, opisujemo varijable procesa koje mogu utjecati na prinos. Naposljetku, predlažemo metode ekstrakcije i analize dobivene propionske kiseline, te ističemo strategije pomoću kojih se mogu zaobići ekonomska ograničenja procesa uzrokovana kompeticijom mikroorganizama tijekom proizvodnje. Na koncentraciju propionske kiseline i produktivnost procesa utječe više čimbenika, kao što su: sastav podloge, tip i veličina bioreaktora te pH-vrijednost i temperatura kao najvažniji čimbenici. Objavljen je velik broj radova o proizvodnji propionske kiseline iz glukoze, permeata sirutke, glicerola, mliječne kiseline, hemiceluloze, hidrolizirane sačme kukuruznih klica, laktoze, melase šećerne trske, te brašna od cjelovitog zrna pšenice hidroliziranog pomoću enzima, ali vrlo mali broj revijalnih prikaza procjenjuje biotehnološke aspekte proizodnje, odnosno varijable bioprocesa

Sensitive leptospira DNA detection using tapered optical fiber sensor

This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations