Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation.

Since the first attempts to use standardised methods for sampling induced airways sputum, two methods for processing the expectorate have evolved. The first involves selecting all viscid or denser portions from the expectorated sample with the aid of an inverted microscope. This method has been extensively evaluated and reported in detail. The second approach involves processing the entire expectorate, comprising sputum plus variable amounts of saliva. Recent modifications to this method include collecting saliva and sputum separately in order to reduce salivary contamination. Both methods have advantages and disadvantages. The advantages of using selected sputum are: squamous cell contamination is v5%, making cell counting easier and quicker to perform, the total cell count (TCC) can be expressed per gram of lower airway secretions, and concentrations of chemicals in the fluid phase are unaffected by the confounding influence of saliva, and can be accurately corrected for dilution. The disadvantage is that selection takes a few minutes longer to perform and requires an inverted microscope. The advantage of using the entire expectorate is that the technique is quicker to perform, but there are some disadvantages that require consideration. The expectorate contains a variable mixture of sputum plus saliva which maydilute the sputum and confound its analysis. The reproducibility of cell counts has been reported to be lower if squamous cell contamination represents w20% of all recovered cells. There is conflicting data as to whether or not differential cell counts (DCCs) differ between the two methods. One study reported a higher percentage of eosinophils in sputum processed by the selection method compared to the entire expectorate but this has not been confirmed in other studies. Although, both the selected sputum and the entire expectorate methods have the same ability to distinguish asthmatics or bronchitics from healthy subjects, they are not interchangeable, and, once a technique has been adopted for a given study, it should always be applied

Broad band X-ray spectral properties of Gamma-ray bursts with BeppoSAX

In about one year, five gamma-ray bursts were simultaneously observed with the Wide Field Cameras and Gamma Ray Burst Monitor aboard the BeppoSAX satellite. From some of them X-ray afterglow emission has been clearly detected with the same satellite. In order to understand how GRB emission is related to the X-ray afterglow, we are performing a systematic study of the spectral properties of these events. We report here preliminary results of this study.Comment: 5 pages, 3 figures. To appear in the Proceedings of the 4th Huntsville Gamma-ray Burst Symposiu

Gamma-Ray Burst 980329 and its X-Ray Afterglow

GRB 980329 is the brightest gamma-ray burst detected so far with the Wide Field Cameras aboard BeppoSAX, both in gamma-rays and X-rays. With respect to its fluence (2.6 X 10**-5 erg/s/cm**2 in 50 to 300 keV) it would be in the top 4% of gamma-ray bursts in the 4B catalog (Meegan et al. 1998). The time-averaged burst spectrum from 2 to 20 and 70 to 650 keV can be well described by the empirical model of Band et al. (1993). The resulting photon index above the break energy is exceptionally hard at -1.32 +/- 0.03. An X-ray afterglow was detected with the narrow-field instruments aboard BeppoSAX 7 h after the event within the error box as determined with the Wide Field Cameras. Its peak flux is (1.4 +/- 0.2) X 10**-12 erg/s/cm**2 (2 to 10 keV). The afterglow decayed according to a power law function with an index of -1.35 +/- 0.03. GRB 980329 is characterized by being bright and hard, and lacking strong spectral evolution.Comment: 13 pages with 3 figures. Accepted for publication in ApJ Let