Neural and Aneural Regions Generated by the Use of Chemical Surface Coatings

The disordered environment found in conventional neural cultures impedes various applications where cell directionality is a key process for functionality. Neurons are highly specialized cells known to be greatly dependent on interactions with their surroundings. Therefore, when chemical cues are incorporated on the surface material, a precise control over neuronal behavior can be achieved. Here, the behavior of SH-SY5Y neurons on a variety of self-assembled monolayers (SAMs) and polymer brushes both in isolation and combination to promote cellular spatial control was determined. APTES and BIBB coatings promoted the highest cell viability, proliferation, metabolic activity, and neuronal maturation, while low cell survival was seen on PKSPMA and PMETAC surfaces. These cell-attractive and repulsive surfaces were combined to generate a binary BIBB-PKSPMA coating, whereby cellular growth was restricted to an exclusive neural region. The utility of these coatings when precisely combined could act as a bioactive/bioinert surface resulting in a biomimetic environment where control over neuronal growth and directionality can be achieved

Search for the electric dipole moment of the electron with thorium monoxide

The electric dipole moment of the electron (eEDM) is a signature of CP-violating physics beyond the Standard Model. We describe an ongoing experiment to measure or set improved limits to the eEDM, using a cold beam of thorium monoxide (ThO) molecules. The metastable $H {}^3\Delta_1$ state in ThO has important advantages for such an experiment. We argue that the statistical uncertainty of an eEDM measurement could be improved by as much as 3 orders of magnitude compared to the current experimental limit, in a first-generation apparatus using a cold ThO beam. We describe our measurements of the $H$ state lifetime and the production of ThO molecules in a beam, which provide crucial data for the eEDM sensitivity estimate. ThO also has ideal properties for the rejection of a number of known systematic errors; these properties and their implications are described.Comment: v2: Equation (11) correcte

Mechanical Faraday effect for orbital angular momentum-carrying beams

When linearly polarised light is transmitted through a spinning window, the plane of polarisation is rotated. This rotation arises through a phase change that is applied to the circularly polarised states corresponding to the spin angular momentum (SAM). Here we show an analogous effect for the orbital angular momentum (OAM), where a differential phase between the positive and negative modes (±ℓ) is observed as a rotation of the transmitted image. For normal materials, this rotation is on the order of a micro radian, but by using a slow-light medium, we show a rotation of a few degrees. We also note that, within the bounds of our experimental parameters, this rotation angle does not exceed the scale of the spatial features in the beam profile

In-situ velocity imaging of ultracold atoms using slow--light

The optical response of a moving medium suitably driven into a slow-light propagation regime strongly depends on its velocity. This effect can be used to devise a novel scheme for imaging ultraslow velocity fields. The scheme turns out to be particularly amenable to study in-situ the dynamics of collective and topological excitations of a trapped Bose-Einstein condensate. We illustrate the advantages of using slow-light imaging specifically for sloshing oscillations and bent vortices in a stirred condensate

Measurement of the electron electric dipole moment using YbF molecules

The most sensitive measurements of the electron electric dipole moment d_e have previously been made using heavy atoms. Heavy polar molecules offer a greater sensitivity to d_e because the interaction energy to be measured is typically 10^3 times larger than in a heavy atom. We report the first measurement of this kind, for which we have used the molecule YbF. Together, the large interaction energy and the strong tensor polarizability of the molecule make our experiment essentially free of the systematic errors that currently limit d_e measurements in atoms. Our first result d_e = (- 0.2 \pm 3.2) x 10^-26 e.cm is less sensitive than the best atom measurement, but is limited only by counting statistics and demonstrates the power of the method.Comment: 4 pages, 4 figures. v2. Minor corrections and clarifications made in response to referee comment

Mechanical Design of a Microwave Imaging Device for Breast Cancer Detection in MRI Scanners

This project seeks to develop an updated version of a microwave imaging device for use in conjunction with breast MRI, improving upon existing technology and developing novel concepts for the device. It posits three primary redesign targets for updating the previous system: resizing the system height, making the device more iteration- friendly, and improving the overall manufacturability of the device by replacing custom components with commercially available alternatives. All three of these redesign targets are met in the new design, V2.0. The height is reduced by reducing antenna travel and height, embedding some components, and shortening the tank wall, resulting in 4.65 cm of height savings from the previous version. The hand-machined acrylic plates are replaced by laminated, laser cut acrylic pieces to increase ease of prototyping, meeting the second target. The worm, worm wheel, lead screw, and lead nut are replaced with commercial components to improve manufacturability. To develop the novel components, extensive torque testing is performed to quantify the system torque requirements, yielding a maximum torque expectation of 0.6 N-m. A pneumatic motor is selected with a minimum torque rating of 2 N-m for rated pressure, meeting the minimum safety factor of 2 for the torque specification. A carbon fiber drive shaft and support mechanism are designed to ensure patient safety around the motor and prevent degradation of the images while in the MRI suite. Finally, a patient bed design is presented to house the device, shield the motor, and provide a platform for the patient to lie on. Preliminary testing results indicate that all the redesign targets are met and demonstrate that the gearing system is more robust and that the novel components all have merit for further development and exploration. Ex situ mechanical testing provides a verification that the components are functioning as expected and provides a way to tune the controller outside of the MRI suite. The next step for the project is to perform a phantom trial to ensure that the mechanical system functions in situ as expected, and pending a successful result, moving to a clinical trial with volunteer patients

Transverse Fresnel-Fizeau drag effects in strongly dispersive media

A light beam normally incident upon an uniformly moving dielectric medium is in general subject to bendings due to a transverse Fresnel-Fizeau light drag effect. In conventional dielectrics, the magnitude of this bending effect is very small and hard to detect. Yet, it can be dramatically enhanced in strongly dispersive media where slow group velocities in the m/s range have been recently observed taking advantage of the electromagnetically induced transparency (EIT) effect. In addition to the usual downstream drag that takes place for positive group velocities, we predict a significant anomalous upstream drag to occur for small and negative group velocities. Furthermore, for sufficiently fast speeds of the medium, higher order dispersion terms are found to play an important role and to be responsible for peculiar effects such as light propagation along curved paths and the restoration of the spatial coherence of an incident noisy beam. The physics underlying this new class of slow-light effects is thoroughly discussed

Limits on the monopole magnetic field from measurements of the electric dipole moments of atoms, molecules and the neutron

A radial magnetic field can induce a time invariance violating electric dipole moment (EDM) in quantum systems. The EDMs of the Tl, Cs, Xe and Hg atoms and the neutron that are produced by such a field are estimated. The contributions of such a field to the constants, $\chi$ of the T,P-odd interactions $\chi_e {\bf N} \cdot {\bf s}/s$ and $\chi_N {\bf N} \cdot {\bf I}/I$ are also estimated for the TlF, HgF and YbF molecules (where ${\bf s}$ (${\bf I}$) is the electron (nuclear) spin and ${\bf N}$ is the molecular axis). The best limit on the contact monopole field can be obtained from the measured value of the Tl EDM. The possibility of such a field being produced from polarization of the vacuum of electrically charged magnetic monopoles (dyons) by a Coulomb field is discussed, as well as the limit on these dyons. An alternative mechanism involves chromomagnetic and chromoelectric fields in QCD.Comment: Uses RevTex, 16 pages, 4 postscript figures. An explanation of why there is no orbital contribution to the EDM has been added, and the presentation has been improved in genera

Programmable in situ amplification for multiplexed imaging of mRNA expression

In situ hybridization methods enable the mapping of mRNA expression within intact biological samples. With current approaches, it is challenging to simultaneously map multiple target mRNAs within whole-mount vertebrate embryos, representing a significant limitation in attempting to study interacting regulatory elements in systems most relevant to human development and disease. Here, we report a multiplexed fluorescent in situ hybridization method based on orthogonal amplification with hybridization chain reactions (HCR). With this approach, RNA probes complementary to mRNA targets trigger chain reactions in which fluorophore-labeled RNA hairpins self-assemble into tethered fluorescent amplification polymers. The programmability and sequence specificity of these amplification cascades enable multiple HCR amplifiers to operate orthogonally at the same time in the same sample. Robust performance is achieved when imaging five target mRNAs simultaneously in fixed whole-mount and sectioned zebrafish embryos. HCR amplifiers exhibit deep sample penetration, high signal-to-background ratios and sharp signal localization

IntEgrating Smoking Cessation treatment As part of usual Psychological care for dEpression and anxiety (ESCAPE): protocol for a randomised and controlled, multicentre, acceptability, feasibility and implementation trial

Background People with depression/anxiety are twice as likely to smoke and are less responsive to standard tobacco treatments, leading to a reduced life expectancy of up to  13.6 years compared to people without depression/anxiety. However, this group of smokers is motivated to quit, and as a result of quitting smoking, their depression/anxiety is likely to improve. In England, people with depression/anxiety are referred to a primary care-based psychological therapies service known as ‘Improving Access to Psychological Therapies’ (IAPT), which could offer smoking cessation treatment as part of usual care but currently does not. In this study, we aim (1) to establish the feasibility and acceptability of delivering a smoking cessation treatment alongside IAPT usual care and (2) to establish the feasibility of a multi-centre randomised trial to compare the combined smoking cessation and IAPT treatment to usual IAPT treatment alone. Methods A randomised and controlled, multi-centre trial to test the acceptability, feasibility and implementation of smoking cessation treatment as offered alongside usual IAPT care, compared to usual care alone, with nested qualitative methods. We will include adult daily smokers with depression/anxiety, who would like help to quit smoking and are about to start IAPT treatment. Follow-up will be conducted at 3-months after baseline. The main outcome will be retention in the smoking cessation treatment. Secondary outcomes are smoking-related (biochemically-verified 7-day point prevalence smoking cessation, number of cigarettes smoked per day, Heaviness of Smoking Index), mental health-related (PHQ-9), service-related (number of ‘Did Not Attends’, number of planned and completed IAPT sessions), acceptability and feasibility (participant and clinician acceptability and satisfaction of intervention as assessed by questionnaires and qualitative interviews, interviews will also explore acceptability and feasibility of data collection procedures and impact of smoking cessation treatment on usual care and mental health recovery) and implementation-related (intervention delivery checklist, qualitative analysis of intervention delivery). Discussion If the intervention is shown to be acceptable, feasible and suitably implemented, we can conduct a randomised controlled trial. In a future trial, we would examine whether adding smoking cessation treatment increases smoking abstinence and improves depression and anxiety more than usual care, which would lead to long-term health improvement