Property Rights and Public Resources

4 pages

Severe muscle trauma triggers heightened and prolonged local musculoskeletal inflammation and impairs adjacent tibia fracture healing

Objectives: Complicated fracture healing is often associated with the severity of surrounding muscle tissue trauma. Since inflammation is a primary determinant of musculoskeletal health and regeneration, it is plausible that delayed healing and non-unions are partly caused by compounding local inflammation in response to concomitant muscle trauma. Methods and results: To investigate this possibility, a Lewis rat open fracture model [tibia osteotomy with adjacent tibialis anterior (TA) muscle volumetric muscle loss (VML) injury] was interrogated. We observed that VML injury impaired tibia healing, as indicated by diminished mechanical strength and decreased mineralized bone within the fracture callus, as well as continued presence of cartilage instead of woven bone 28 days post-injury. The VML injured muscle presented innate and adaptive immune responses that were atypical of canonical muscle injury healing. Additionally, the VML injury resulted in a perturbation of the inflammatory phase of fracture healing, as indicated by elevations of CD3+ lymphocytes and CD68+ macrophages in the fracture callus at 3 and 14d post-injury, respectively. Conclusions: These data indicate that heightened and sustained innate and adaptive immune responses to traumatized muscle are associated with impaired fracture healing and may be targeted for the prevention of delayed and non-union following musculoskeletal trauma

DESK ORGANIZER YANG DILENGKAPI DENGAN SINAR ULTRAVIOLET UNTUK TELLER BANK

With the COVID-19 pandemic, people are afraid to meet other people because they are afraid of getting infected, as well as people who work as tellers, where these tellers meet other people every day. In order to reduce the fear and worry of the people, the idea emerged to design a desk organizer equipped with ultraviolet-C where this ultraviolet-C light can also kill COVID-19 with a certain spectrum. The study was carried out by conducting interviews with several tellers and the conclusion was that there was still not much use of ultraviolet-C rays in banks to sterilize goods, where cleanliness of goods was very important during this COVID-19, the use of ultraviolet-C rays is still being used. to check the authenticity of the money. The innovation that he wants to produce is the use of ultraviolet-C rays that can sterilize goods where there are still not many uses of ultraviolet-C rays in banks. On the other hand, tellers also need a desk organizer so that the table is always neat, therefore the innovation is designed, namely a desk organizer equipped with a slot to sterilize goods using Ultraviolet-C rays with the aim of not only keeping the table tidy, it can also be used to disconnect the chain of spread of COVID-19 that can stick to goods

Virtues of the self : ethics and the critique of feminist identity politics

This thesis is situated at the intersection of feminist political theory, identity politics and moral philosophy. Its broader aim is to show the positive consequences of returning the self and its inner activity to the ethical domain for feminist identity politics. To this end, it brings feminist identity politics into dialogue with contemporary developments in virtue ethics, in particular Christine Swanton’s pluralistic virtue ethics. As its starting point, it takes issue with the tendency to reduce the complexity of identity to issues of category. The first part of the thesis problematises this tendency and argues for a reconsideration of the question of identity politics by shifting the focus away from identity per se and towards a more complex picture of the self that is reflective of the constitutive relation between the self and identifications, commitments and values. The work of the post-modern feminists Wendy Brown and Judith Butlers are read as proposing just such a shift away from the identitarian engagement of identity politics of ‘who am I?’ towards a more ethically imbued engagement that centres a complex self with inner depths. Part Two of the thesis extends this reconceptualisation of the problematic of identity politics and elaborates on what it could mean to undertake such a shift and how such a project could be conceived. Drawing on both Michael Sandel’s and Michel Foucault’s formulations of the self, identity and its relation to the good, the thesis develops the argument that the problematic of identity politics, articulated in ethical language, enables the formulation of an argument for giving an account of the good life and that this entails developing a subject imbued with a full inner life. Part Three of the thesis argues that contemporary work in virtue ethics offers the best way to take this project forward, suggesting that it represents a positive development in conceptions of the self and that a complex picture of the person emerges that provides the basis for a richer approach to the ethical concerns raised in identity politics. The thesis concludes by illustrating the potential value of taking those feminist insights into the constructed nature of identity into dialogue with a pluralistic virtue ethical account of the self and suggests that this approach provides new opportunities for understanding and discussing the collective dimension of identity politics in situations of diversity and inequality

Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion

BACKGROUND: Chronic, non-healing wounds are often characterized by the persistence of bacteria within biofilms - aggregations of cells encased within a self-produced polysaccharide matrix. Biofilm bacteria exhibit unique characteristics from planktonic, or culture-grown, bacterial phenotype, including diminished responses to antimicrobial therapy and persistence against host immune responses. Mesenchymal stromal cells (MSCs) are host cells characterized by their multifunctional ability to undergo differentiation into multiple cell types and modulation of host-immune responses by secreting factors that promote wound healing. While these characteristics make MSCs an attractive therapeutic for wounds, these pro-healing activities may be differentially influenced in the context of an infection (i.e., biofilm related infections) within chronic wounds. Herein, we evaluated the effect of soluble factors derived from biofilms of clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa on the viability, differentiation, and paracrine activity of human MSCs to evaluate the influence of biofilms on MSC activity in vitro. RESULTS: Exposure of MSCs to biofilm-conditioned medias of S. aureus and P. aeruginosa resulted in reductions in cell viability, in part due to activation of apoptosis. Similarly, exposure to soluble factors from biofilms was also observed to diminish the migration ability of cells and to hinder multi-lineage differentiation of MSCs. In contrast to these findings, exposure of MSCs to soluble factors from biofilms resulted in significant increases in the release of paracrine factors involved in inflammation and wound healing. CONCLUSIONS: Collectively, these findings demonstrate that factors produced by biofilms can negatively impact the intrinsic properties of MSCs, in particular limiting the migratory and differentiation capacity of MSCs. Consequently, these studies suggest use/application of stem-cell therapies in the context of infection may have a limited therapeutic effect. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-015-0412-x) contains supplementary material, which is available to authorized users

Genetic parameters for faecal egg count, packed-cell volume and body-weight in Santa Inês lambs

Worm infection is one of the main factors responsible for economic losses in sheep breeding in Brazil. Random regression analysis was used to estimate genetic parameters for the factors faecal egg-count (FEC), packed-cell volume (PCV) and body weight (BW) in Santa Inês lambs. Data from 119 female, offspring of nine rams, were collected between December, 2005 and December, 2006, from the experimental flock of Embrapa Tabuleiros Costeiros, the Brazilian Agricultural Research Corporation located in Frei Paulo, SE, Brazil. After weaning, females were drenched until the faecal egg count had dropped to zero. Two natural challenges were undertaken. FEC heritability was extremely variable, this increasing from 0.04 to 0.27 in the first challenge and from 0.01 to 0.52 during the second. PCV heritability peaks were 0.31 and 0.12 in the first and second challenges, respectively. In the second challenge, BW heritability was close to 0.90. The genetic correlations among these traits did not differ from zero. There is the possibility of increasing parasite resistance in Santa Inês by selecting those animals with lower FEC. Selection to increase resistance will not adversely affect lamb-growth, although lambs with a slow growth-rate may be more susceptible to infection

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants

The omentum is a visceral adipose tissue rich in fat-associated lymphoid clusters (FALCs) that collects peritoneal contaminants and provides a first layer of immunological defense within the abdomen. Here, we investigated the mechanisms that mediate the capture of peritoneal contaminants during peritonitis. Single-cell RNA sequencing and spatial analysis of omental stromal cells revealed that the surface of FALCs were covered by CXCL1+ mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants

Genome-wide associations for fertility traits in Holstein–Friesian dairy cows using data from experimental research herds in four European countries

peer-reviewedGenome-wide association studies for difficult-to-measure traits are generally limited by the sample population size with accurate phenotypic data. The objective of this study was to utilise data on primiparous Holstein–Friesian cows from experimental farms in Ireland, the United Kingdom, the Netherlands and Sweden to identify genomic regions associated with traditional measures of fertility, as well as a fertility phenotype derived from milk progesterone profiles. Traditional fertility measures investigated were days to first heat, days to first service, pregnancy rate to first service, number of services and calving interval (CI); post-partum interval to the commencement of luteal activity (CLA) was derived using routine milk progesterone assays. Phenotypic and genotypic data on 37 590 single nucleotide polymorphisms (SNPs) were available for up to 1570 primiparous cows. Genetic parameters were estimated using linear animal models, and univariate and bivariate genome-wide association analyses were undertaken using Bayesian stochastic search variable selection performed using Gibbs sampling. Heritability estimates of the traditional fertility traits varied from 0.03 to 0.16; the heritability for CLA was 0.13. The posterior quantitative trait locus (QTL) probabilities, across the genome, for the traditional fertility measures were all ,0.021. Posterior QTL probabilities of 0.060 and 0.045 were observed for CLA on SNPs each on chromosome 2 and chromosome 21, respectively, in the univariate analyses; these probabilities increased when CLA was included in the bivariate analyses with the traditional fertility traits. For example, in the bivariate analysis with CI, the posterior QTL probability of the two aforementioned SNPs were 0.662 and 0.123. Candidate genes in the vicinity of these SNPs are discussed. The results from this study suggest that the power of genome-wide association studies in cattle may be increased by sharing of data and also possibly by using physiological measures of the trait under investigation.European Union Seventh Research Framework Programme (Grant Agreement KBBE-211708, RobustMilk project