Economic evaluation of apple harvest and in-filed sorting technology

The U.S. apple industry, which generated more than $2.7 billion in revenue at the farm gate in 2013, is facing critical challenges with decreased availability of labor and increased labor and production costs. To address these challenges, a self-propelled apple harvest and in-field sorting machine is being developed in our laboratory. This article reports on the economic evaluation of this prototype machine by considering machine cost (annual ownership and operating costs), harvest productivity increase (including that due to decreased occupational injuries), and cost savings in postharvest storage and packing resulting from in-field sorting of fresh market quality apples from processing apples for both fresh apple growers and processing apple growers. The economic evaluation was conducted based on the assumptions that the machine increases harvest productivity by 43 from$13,500 to $78,400 when the machine price is between$100,000 and $160,000. For processing apple orchards with processing apple incidences of 80$23,900 to $81,700. Overall, the benefits gained from in-field sorting outweigh those from the harvest productivity increase, and integration of the harvest-assist and in-field sorting functions is more beneficial to apple growers. This technology will help the U.S. apple industry improve labor productivity and reduce production costs, and thus it looks promising for commercialization

Anesthesiologists\u27 Preferences regarding Visitor Presence during Placement of Neuraxial Labor Analgesia

Introduction: Neuraxial labor analgesia has become an integral part of modern obstetric anesthetic practice. Presence of a familiar person during its placement may be beneficial to the patient. A survey was sent to anesthesiologists practicing obstetric anesthesia in the USA to determine their views. Methods: The survey queried the following: existence of a written policy; would they allow a visitor; visitor\u27s view, sitting or standing; reasons to allow or not allow a visitor; and influence by other staff on the decision. The responses were analyzed using multiple chi-square analyses. Results: Most practitioners supported allowing a visitor during placement. Reduction of patient anxiety and fulfillment of patient request were the major reasons for allowing a visitor. Sitting position and no view of the workspace were preferred. Visitor interference and safety were cited as the major reasons for precluding a visitor. Nonanesthesia providers rarely influenced the decision. Epidural analgesia was the preferred technique. Essentially no bias was found in the responses; there was statistical uniformity regardless of procedures done per week, years in practice, professional certification, geographic region (rural, urban, or suburban), or academic, private, or government responders. Conclusion: The practice of visitor presence during the placement of neuraxial labor analgesia is gaining acceptance

A review of bin filling technologies for apple harvest and postharvest handling

Bin fillers play a critical role in transporting and distributing fruit evenly and without bruising into individual bins or containers from harvest platforms infield or sorting lines in the packinghouse. Over the years, a large variety of bin fillers have been developed for infield use and postharvest handling. This article reviews different bin filling technologies in terms of major design features, performance, and throughput, as well as automatic control and safety features. For infield use, bin filler designs have evolved from the early use of conveyors and reciprocating plates to recent adoption of soft pads and foam rollers, to reduce bruising and improve fruit distributions. For postharvest use, tilted bins and conveyors commonly used with early bin fillers have been replaced with hinged trays and vacuum suction cups for fruit transport and cylinder brushes and swingable dividers for bruise prevention. While many types of bin fillers have been developed, few are suitable for infield use because it imposes more constraints than postharvest use. Despite the use of automatic sensing and control in most bin fillers, human assistance is still needed during their operation. There are still major issues with the current bin fillers, such as large size, complexity in design, uneven fruit distributions, and low throughput. Further effort should, therefore, be directed towards the development of high throughput, simple yet reliable, compact and fully automated, or even intelligent bin fillers for infield and postharvest use

Development and preliminary evaluation of a new bin filler for apple harvesting and in-filed sorting machine

The bin filler, which is used for filling the fruit container or bin with apples coming from the sorting system, plays a critical role in the self-propelled apple harvest and in-field sorting (HIS) machine that is being developed in our laboratory. Two major technical challenges in developing the bin filler are limited space in the HIS machine and high throughput. A literature review showed that despite many different types of bin fillers currently available for in-field use, none of them is suitable for the HIS machine because of their large size, use of the bin rotating design concept, and high unit cost. Effort has thus been made on the development of new bin filling technology for use with the HIS machine. The new bin filler mainly consists of a mechanical system with a pinwheel design and an automatic control system. A key innovation in the mechanical system is the use of two foam rollers to catch freely falling apples, which has greatly simplified the bin filler design and also made the system compact and lower in cost. The control system is mainly composed of an onboard Arduino microcontroller and three sensors (one infrared sensor and two Hall effect sensors) to monitor and measure the status of apples filling the bin as well as the rotational speed of the pinwheel. A program was developed for the Arduino microcontroller to record and process the data from the sensors in real-time to achieve automatic control of the bin filling. Laboratory tests with ‘Gala’ apples demonstrated that 97% of apples that had been handled by the new bin filler were rated Extra Fancy grade, and its performance exceeded the industry’s requirement for bruising damage to apples

Modulation of the <i>Neisseria gonorrhoeae </i>drug efflux conduit MtrE

We acknowledge funding through the Wellcome Trust Interdisciplinary Research Funds (grant WT097818MF), the Scottish Universities’ Physics Alliance (SUPA), Tenovus Tayside (grant T16/30) and the Tayside Charitable Trust. O.N.V. has been funded through a BBSRC CASE award (BB/J013072/1).Widespread antibiotic resistance, especially of Gram-negative bacteria, has become a severe concern for human health. Tripartite efflux pumps are one of the major contributors to resistance in Gram-negative pathogens, by efficiently expelling a broad spectrum of antibiotics from the organism. In Neisseria gonorrhoeae, one of the first bacteria for which pan-resistance has been reported, the most expressed efflux complex is MtrCDE. Here we present the electrophysiological characterisation of the outer membrane component MtrE and the membrane fusion protein MtrC, obtained by a combination of planar lipid bilayer recordings and in silico techniques. Our in vitro results show that MtrE can be regulated by periplasmic binding events and that the interaction between MtrE and MtrC is sufficient to stabilize this complex in an open state. In contrast to other efflux conduits, the open complex only displays a slight preference for cations. The maximum conductance we obtain in the in vitro recordings is comparable to that seen in our computational electrophysiology simulations conducted on the MtrE crystal structure, indicating that this state may reflect a physiologically relevant open conformation of MtrE. Our results suggest that the MtrC/E binding interface is an important modulator of MtrE function, which could potentially be targeted by new efflux inhibitors.Publisher PDFPeer reviewe

The role of chaperone-mediated autophagy in bortezomib resistant multiple myeloma

Background: Multiple myeloma (MM) remains incurable despite high-dose chemotherapy, autologous stem cell transplants and novel agents. Even with the improved survival of MM patients treated with novel agents, including bortezomib (Bz), the therapeutic options in relapsed/refractory MM remain limited. The majority of MM patients eventually develop resistance to Bz, although the mechanisms of the resistance are poorly understood. Methods: Lysosomal associated membrane protein 2A (LAMP2A) mRNA and protein expression levels were assessed in ex vivo patient samples and a Bz-resistant MM cell line model by in real-rime PCR, western blotting and immunohistochemistry. In vitro modelling of chaperone-mediated autophagy (CMA) activity in response to ER stress were assessed by western blotting and confocal microscopy. The effects of CMA inhibition on MM cell via-bility and Bz sensitivity in MM cells were assessed by Annexin V/7AAD apoptosis assays using flow cytometry. Results: In this study, there is evidence that CMA, a chaperone-mediated protein degradation pathway, is upregulated in Bz-resistant MM and the inhibition of CMA sensitises resistant cells to Bz. The protein levels of LAMP2A, the rate-limiting factor of the CMA pathway, are significantly increased in MM patients resistant to Bz and within our Bz-resistant cell line model. Bz-resistant cell lines also possessed higher basal CMA activity than the Bz-sensitive parent cell line. In MM cell lines, CMA activity was upregulated in response to ER stress induced by Bz. The inhibition of CMA sensi-tises Bz-resistant cells to Bz and the combination of CMA inhibition and Bz in vitro had a more cyto-toxic effect on myeloma cells than Bz alone. Conclusion: In summary, the upregulation of CMA is a potential mechanism of resistance to Bz and a novel target to overcome Bz-resistant MM

A Role for Noncoding Variation in Schizophrenia

A large portion of common variant loci associated with genetic risk for schizophrenia reside within noncoding sequence of unknown function. Here, we demonstrate promoter and enhancer enrichment in schizophrenia variants associated with expression quantitative trait loci (eQTL). The enrichment is greater when functional annotations derived from the human brain are used relative to peripheral tissues. Regulatory trait concordance analysis ranked genes within schizophrenia genome-wide significant loci for a potential functional role, based on colocalization of a risk SNP, eQTL, and regulatory element sequence. We identified potential physical interactions of noncontiguous proximal and distal regulatory elements. This was verified in prefrontal cortex and -induced pluripotent stem cell-derived neurons for the L-type calcium channel (CACNA1C) risk locus. Our findings point to a functional link between schizophrenia-associated noncoding SNPs and 3D genome architecture associated with chromosomal loopings and transcriptional regulation in the brain

Structural basis for nutrient acquisition by dominant members of the human gut microbiota

The human large intestine is populated by a high density of microorganisms, collectively termed the colonic microbiota, which has an important role in human health and nutrition. The survival of microbiota members from the dominant Gram-negative phylum Bacteroidetes depends on their ability to degrade dietary glycans that cannot be metabolized by the host. The genes encoding proteins involved in the degradation of specific glycans are organized into co-regulated polysaccharide utilization loci, with the archetypal locus sus (for starch utilisation system) encoding seven proteins, SusA-SusG. Glycan degradation mainly occurs intracellularly and depends on the import of oligosaccharides by an outer membrane protein complex composed of an extracellular SusD-like lipoprotein and an integral membrane SusC-like TonB-dependent transporter. The presence of the partner SusD-like lipoprotein is the major feature that distinguishes SusC-like proteins from previously characterized TonB-dependent transporters. Many sequenced gut Bacteroides spp. encode over 100 SusCD pairs, of which the majority have unknown functions and substrate specificities. The mechanism by which extracellular substrate binding by SusD proteins is coupled to outer membrane passage through their cognate SusC transporter is unknown. Here we present X-ray crystal structures of two functionally distinct SusCD complexes purified from Bacteroides thetaiotaomicron and derive a general model for substrate translocation. The SusC transporters form homodimers, with each β-barrel protomer tightly capped by SusD. Ligands are bound at the SusC-SusD interface in a large solvent-excluded cavity. Molecular dynamics simulations and single-channel electrophysiology reveal a 'pedal bin' mechanism, in which SusD moves away from SusC in a hinge-like fashion in the absence of ligand to expose the substrate-binding site to the extracellular milieu. These data provide mechanistic insights into outer membrane nutrient import by members of the microbiota, an area of major importance for understanding human-microbiota symbiosis

Global wealth disparities drive adherence to COVID-safe pathways in head and neck cancer surgery

Peer reviewe