Аpplication of the laccase, produced on coconut flesh by Pleurotus Florida for dye decolorization

We investigated the ability of Pleurotus florida to produce laccase on coconut flesh as a solid substrate fermentation. The decolorization of two structurally different dyes such as azo (Reactive Blue 198) and triphenylmethane dye (Malachite Green) were analysed. The decolorization of Reactive blue 198 and Malachite Green at 8 hrs was 93% and 63% respectiely. The untreated and treated dye was characterized by UV-Vis and fourier transform infrared (FTIR) spectroscopy scan. FTIR analysis pointed out the involvement of alkene (C = C) and carboxylic (C – O) groups in the decolorization process. The toxicity with respect to Allium cepa root inhibition was measured to demonstrate the potential of laccase in the detoxication and bioremediation process.The Corresponding author (Principal Investigator – Project No.: BT/PR8973/GBD/27/57/2006 Dt 14/08/07) is grateful to Government of India, Ministry of Science and Technology, Department of Biotechnology for financial support

Factorial design for optimization of laccase production from <i style="mso-bidi-font-style:normal">Pleurotus ostreatus</i> IMI 395545 and laccase mediated synthetic dye decolorization<i style="mso-bidi-font-style:normal"> </i>

236-245In the present study, Plackett–Burman design (PBD) followed by response surface methodology (RSM) was used to optimize the culture medium composition for laccase production from Pleurotus ostreatus IMI 395545. Influence of carbon, nitrogen (sources) and inducer were evaluated by Plackett–Burman design. The factors that induce positive effect on enzyme production were further optimized using central composite design of RSM. The multiple regression equation was used to optimize the values of carbon, nitrogen and inducer for maximum enzyme production. The optimized values were found to be 1.8 g L-1, 0.76 g L-1 and 164.95 µM for glucose, ammonium nitrate and copper sulphate, respectively. The maximum enzyme activity of 2.7 U mL-1 obtained experimentally was very close to the predicted response, 2.5 U mL-1. Furthermore, optimized laccase was used for decolorization of synthetic dyes, such as, reactive blue 172 and reactive red 22, which are routinely used in textile industries. Fourier transform infrared spectroscopy was performed to confirm the decolorization of the studied dyes by laccase. <span style="font-size:9.0pt;mso-fareast-font-family:Calibri;color:black; mso-ansi-language:EN-US" lang="EN-US">Allium cepa <span style="font-size:9.0pt;mso-fareast-font-family:Calibri;color:black;mso-ansi-language: EN-US" lang="EN-US">toxicity test was performed, which indicated that root growth inhibition was dependent upon the concentration of dye. </span

Аpplication of the laccase, produced on coconut flesh by Pleurotus Florida for dye decolorization

We investigated the ability of Pleurotus florida to produce laccase on coconut flesh as a solid substrate fermentation. The decolorization of two structurally different dyes such as azo (Reactive Blue 198) and triphenylmethane dye (Malachite Green) were analysed. The decolorization of Reactive blue 198 and Malachite Green at 8 hrs was 93% and 63% respectiely. The untreated and treated dye was characterized by UV-Vis and fourier transform infrared (FTIR) spectroscopy scan. FTIR analysis pointed out the involvement of alkene (C = C) and carboxylic (C – O) groups in the decolorization process. The toxicity with respect to Allium cepa root inhibition was measured to demonstrate the potential of laccase in the detoxication and bioremediation process.The Corresponding author (Principal Investigator – Project No.: BT/PR8973/GBD/27/57/2006 Dt 14/08/07) is grateful to Government of India, Ministry of Science and Technology, Department of Biotechnology for financial support

Beneficial protective effects of 2-allyl amino 4-methyl sulfanyl butyric acid on glucose metabolism and glycoprotein components in streptozotocin induced diabetic rats with molecular modeling

The potential effects of 2-allyl amino 4-methyl sulfanyl butyric acid (AMSB) on the glucose metabolism and glycoprotein components in streptozotocin (STZ) induced experimental diabetic rats were studied.</p