Adaptations in lactate dehydrogenase and its isozymes in aging mammalian myocardium: Interaction of exercise and temperature

The responses of the left and right ventricles (LV and RV) to physical conditioning in cold (25°C) and thermoneutral temperatures (35°C), with special reference to lactate dehydrogenase (LDH) and its isoenzyme profile, were studied in the 2-month (young)- and 12-month (middle-aged)-old rats. Moderate hypertrophy was a common observation irrespective of age, region and swim temperature. LV, however, hypertrophied to a significantly lesser extent in the middle-aged, than the RV. Blood Lactate (La) content showed a decline in the trained rather than their untrained counterparts. LDH activity decreased with age. Swim training induced elevations in the enzyme activity. The isoenzyme profile was suitably and efficiently altered in the LV and RV of trained animals to meet the arising O2 demands. The above adaptations were best seen in the young and in the animals trained at thermoneutral temperatures. Thus it is suggested that young age is very apt for initiation of training programs although middle-age is not so late. Swimming in water near body temperature is emphasised as a more preferred environment to cold water, in order to derive maximal exercise-associated beneficial effects

Dietary vitamin E and physical exercise: II. Antioxidant status and lipofuscin-like substances in aging rat heart

The heart faces a high risk of free radical injury owing to a slow generation of antioxidant (AO) enzymes by its cells. A general decline in this system may be another reason for the development of age-related diseases. Although the correlation between aging and exercise has been studied extensively, these studies have produced conflicting data on the effects of vitamin E on the aging heart, when it is introduced as an intervening factor. To investigate these effects, we determined the activities of antioxidant enzymes (AOEs) such as superoxide dismutase (SOD) and catalase (CAT), lipid peroxidation (LP), lipofuscin (LF)-like autofluorescent substances and vitamin E content in the left and right ventricles (LV and RV) of the heart in male Wistar albino rats of 4-(young adults), 8-(old adults), 12-(middle-age) and 22-mos(old) of age. Animals were orally supplemented with vitamin E and allowed to swim for 30min/day, 5 days/week and for a total period of 60 days. Exercise training in all the age groups except the old was effective in upregulating the SOD activity. Old trainees showed an increase in SOD activity when supplemented with vitamin E. In the 22-mo-olds, a remarkable decrease in CAT activity was seen. Exercise by itself upregulated the CAT as well as SOD activity in all age groups except the old wherein vitamin E was effective in increasing the activities of AOEs. Supplementation significantly reduced LP as evidenced by lowered malondialdehyde (MDA) and LF-like autofluorescent substances in the trained as well as sedentary rats. Tissue vitamin E content was low in the swim trainees that were not supplemented. This change, well emphasized in the trainee groups of 22-mo-old suggests the probable utilization of vitamin E in keeping free radicals at bay. Our results suggest that vitamin E can stand out as a significant tool in ameliorating the declining AO defense in the old rats. © 2002 Elsevier Science Inc. All rights reserved

Temperature-induced changes in anaerobic capacity of interventricular septum in trained young and middle-aged rats

The interventricular site has been proposed as one of the sites for the myocyte cell death with aging and thereby enhancing the possibility of ventricular dysfunction with aging. Variations in lactate dehydrogenase (LDH), a key glycolytic regulatory enzyme along with its isomers were used as indices to evaluate the anaerobic capacity of the interventricular septum (IVS) in 2-month-old (young) and 12-month-old (middle-aged) rats trained to swim at 25°C (cold, C) and 35°C (thermo neutral, N) temperatures. Blood lactate (La) and LDH along with its isoenzymes in the IVS were assessed and compared with untrained and sedentary rats. Increased septal and heart weight was indicative of age-related hypertrophy accompanied by increased septal thickness. Elevated total LDH activity was evident in both the trained groups in the young. The middle-aged animals exhibited lesser variations in LDH and, H and M subunits. The data suggest an efficient adaptability of the IVS of young rats to physical training in contrast to a poor response by the IVS of middle-aged animals

Dietary vitamin E and physical exercise: I. Altered endurance capacity and plasma lipid profile in ageing rats

The effect of vitamin E on the exercise performance and plasma lipid profile was studied in male Wistar rats of 4-(young adults), 8-(old adults), 12-(middle-age) and 22-months (old) of age. Animals were orally supplemented with vitamin E and allowed to swim for 30min/day, 5 days/week and for a total period of 60 days. Swim velocity (Sv), external work done (Wext) and endurance (E) capacity were the parameters that were used to assess the exercise performance of the trained rats that were either supplemented or non-supplemented with the dietary antioxidant. Plasma lipid profile analyses were in terms of low-density lipoprotein (LDL-C), high-density lipoprotein, (HDL-C) cholesterol and total cholesterol (C). Age-related decline in Sv was noticeable in the 22-months old rats. However, the effect of vitamin E on the Sv between the trained groups was not evident in any of the age groups. Wext increased linearly with age with no significant variations between the trainees. Trainee rats, when allowed to swim to exhaustion, showed a higher endurance capacity when supplemented with vitamin E. However, this capacity declined with age. There was a significant age-associated elevation in plasma C with corresponding increase in LDL-C. Exercise training in conjunction with vitamin E supplementation was most effective in elevating HDL-C levels in all age groups. These changes were accompanied by significant reductions in cholesterol/HDL-C ratios in animals receiving vitamin E, sedentary or otherwise. Our data suggests that it may be important to consider vitamin E while attempting to derive the benefits of swim training, both in terms of favorably altering the plasma lipid profile as well as enhancing the endurance capacity of exercise trainees. Dietary supplementation by vitamin E could attenuate the early onset of fatigue in the old. © 2002 Elsevier Science Inc. All rights reserved

Effect of Panchagavya on Growth, Yield and Quality of Tomato (Lycopersicon esculentum L.)

An experiment was conducted to determine the “Effect of Panchagavya on growth, yield and quality of tomato (Lycopersicon esculentum L.)” at Horticulture Research Field, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj (Uttar Pradesh) during Kharif season 2021. Total 12 treatments and 3 Hybrids were replicated thrice in a factorial randomized block design (FRBD). Hybrids are TMTH-234, TMTH-2267 and TMTH-288. These hybrids were treated with different concentrations of Panchagavya (1%, 2%, 3%) and control (water spray) were sprayed at 15, 30, 45 days after transplanting. The application of Panchagavya significantly affects the vegetative as well as reproductive attributes of the crop. The results showed that hybrid TMTH-2267 with 3% spray of Panchagavya performed best in terms of plant height (78.14 cm), days to 50% flowering (71.10), days to first harvest (98.10), polar diameter (5.47 cm), radial diameter (6.90 cm), number of fruits per plant (24), fruit weight (280.08 g), fruit yield per plant (1.68 kg), yield per hectare (55.88 t) and TSS (5.83 0Brix)