Rainfall Variability along the Southern Flank of the Bambouto Mountain(West-Cameroon)

This paper presents the rainfall variability along the southern flank of the Bambouto mountain. Data were collected from rain gauges, while spatial variability was estimated through daily recorded data. Monthly and annual data were used to draw isohyetes via the triangular method, with linear interpolations between observation points. Results show that rainfall is highly variable along the slope. Daily rainfall amounts range from 0.1 mm to 120 mm. Mean yearly rainfall is 1918.1 mm. Rainfall amount does not have a linear relationship with altitude. Dschang is characterised by abnormally high rainfall. Following a North-South direction, rainfall decreases from Dschang to a Melang-Loung-Djuttitsa axis. From this axis, the gradient reverses as rainfall increases rapidly towards the Mélétan mountain. The existence of the relatively dry zone within the hillside seems to be due to the influence of two air masses. The first is cold and very wet which moves from the Mamfe basin to the summit zone where it starts to warm up as it flows towards Melang and Loung where temperature increases. The second comes from the south to south-east monsoon which is also impoverished during the ascension to higher altitudes. It is also likely that a third air mass from the dry harmattan is involved depending on the position of the ITCZ

A systematic review of methods to immobilise breast tissue during adjuvant breast irradiation

Greater use of 3D conformal, Intensity Modulated Radiotherapy (IMRT) and external beam partial breast irradiation following local excision (LE) for breast cancer has necessitated a review of the effectiveness of immobilisation methods to stabilise breast tissue. To identify the suitability of currently available breast (rather than thorax) immobilisation techniques an appraisal of the literature was undertaken. The aim was to identify and evaluate the benefit of additional or novel immobilisation approaches (beyond the standard supine, single arm abducted and angled breast board technique adopted in most radiotherapy departments). A database search was supplemented with an individual search of key radiotherapy peer-reviewed journals, author searching, and searching of the grey literature. A total of 27 articles met the inclusion criteria. The review identified good reproducibility of the thorax using the standard supine arm-pole technique. Reproducibility with the prone technique appears inferior to supine methods (based on data from existing randomised controlled trials). Assessing the effectiveness of additional breast support devices (such as rings or thermoplastic material) is hampered by small sample sizes and a lack of randomised data for comparison. Attention to breast immobilisation is recommended, as well as agreement on how breast stability should be measured using volumetric imaging. Keywords: Breast, immobilisation, positioning, reproducibility, review.</p

Redundant Mesh Trees for Robust Scalable Low-latency P2P Media Distribution

A redundant mesh (RMS) tree structure is proposed as a P2P network topology for reducing network latency. The RMS tree structure may enable low-latency network scalability through redundant connections within tree nodes, and a fully connected mesh cluster at each node of the tree structure. When compared to a traditional P2P or client-server network topology, bandwidth demands may be reduced at every node of the network. Thus, latent network capabilities may be utilized, and overall system latency may decrease

Genomic resolution of a cold subsurface aquifer community provides metabolic insights for novel microbes adapted to high CO2 concentrations.

As in many deep underground environments, the microbial communities in subsurface high-CO2 ecosystems remain relatively unexplored. Recent investigations based on single-gene assays revealed a remarkable variety of organisms from little studied phyla in Crystal Geyser (Utah, USA), a site where deeply sourced CO2 -saturated fluids are erupted at the surface. To provide genomic resolution of the metabolisms of these organisms, we used a novel metagenomic approach to recover 227 high-quality genomes from 150 microbial species affiliated with 46 different phylum-level lineages. Bacteria from two novel phylum-level lineages have the capacity for CO2 fixation. Analyses of carbon fixation pathways in all studied organisms revealed that the Wood-Ljungdahl pathway and the Calvin-Benson-Bassham Cycle occurred with the highest frequency, whereas the reverse TCA cycle was little used. We infer that this, and selection for form II RuBisCOs, are adaptions to high CO2 -concentrations. However, many autotrophs can also grow mixotrophically, a strategy that confers metabolic versatility. The assignment of 156 hydrogenases to 90 different organisms suggests that H2 is an important inter-species energy currency even under gaseous CO2 -saturation. Overall, metabolic analyses at the organism level provided insight into the biochemical cycles that support subsurface life under the extreme condition of CO2 saturation

Rapid inoculation of single bacteria into parallel picoliter fermentation chambers

Probst C, Grünberger A, Braun N, et al. Rapid inoculation of single bacteria into parallel picoliter fermentation chambers. Analytical methods. 2015;7(1):91-98.Microfluidic single-cell cultivation devices have been successfully utilized in a variety of biological research fields. One major obstacle to the successful implementation of high throughput single-cell cultivation technology is the requirement for a simple, fast and reliable cell inoculation procedure. In the present report, an air-bubble-based cell loading methodology is described and validated for inoculating single bacteria into multiple picoliter sized growth chambers arranged in a highly parallel manner. It is shown that the application of the injected air bubble can serve as a reproducible mechanism to modify laminar flow conditions. In this way, convective flow was temporarily induced in more than 1000 cultivation chambers simultaneously, which under normal conditions operate exclusively under diffusive mass transport. Within an inoculation time of 100 s, Corynebacterium glutamicum cells were inoculated by convection at minimal stress level and single bacteria remain successfully trapped by cell-wall interactions. The procedure is easy, fast, gentle and requires only minimal fluidic control and equipment. The technique is well suited for microbial cell loading into commonly used microfluidic growth sites arranged in parallel intended for high throughput single-cell analysis

Radiosynthesis of [18F]-Labelled Pro-Nucleotides (ProTides).

Phosphoramidate pro-nucleotides (ProTides) have revolutionized the field of anti-viral and anti-cancer nucleoside therapy, overcoming the major limitations of nucleoside therapies and achieving clinical and commercial success. Despite the translation of ProTide technology into the clinic, there remain unresolved in vivo pharmacokinetic and pharmacodynamic questions. Positron Emission Tomography (PET) imaging using [18F]-labelled model ProTides could directly address key mechanistic questions and predict response to ProTide therapy. Here we report the first radiochemical synthesis of [18F]ProTides as novel probes for PET imaging. As a proof of concept, two chemically distinct radiolabelled ProTides have been synthesized as models of 3'- and 2'-fluorinated ProTides following different radiosynthetic approaches. The 3'-[18F]FLT ProTide was obtained via a late stage [18F]fluorination in radiochemical yields (RCY) of 15-30% (n = 5, decay-corrected from end of bombardment (EoB)), with high radiochemical purities (97%) and molar activities of 56 GBq/μmol (total synthesis time of 130 min.). The 2'-[18F]FIAU ProTide was obtained via an early stage [18F]fluorination approach with an RCY of 1-5% (n = 7, decay-corrected from EoB), with high radiochemical purities (98%) and molar activities of 53 GBq/μmol (total synthesis time of 240 min)

Polydimethylsiloxane (PDMS) Sub-Micron Traps for Single-Cell Analysis of Bacteria

Probst C, Grünberger A, Wiechert W, Kohlheyer D. Polydimethylsiloxane (PDMS) Sub-Micron Traps for Single-Cell Analysis of Bacteria. Micromachines. 2013;4(4):357-369.Microfluidics has become an essential tool in single-cell analysis assays for gaining more accurate insights into cell behavior. Various microfluidics methods have been introduced facilitating single-cell analysis of a broad range of cell types. However, the study of prokaryotic cells such as Escherichia coli and others still faces the challenge of achieving proper single-cell immobilization simply due to their small size and often fast growth rates. Recently, new approaches were presented to investigate bacteria growing in monolayers and single-cell tracks under environmental control. This allows for high-resolution time-lapse observation of cell proliferation, cell morphology and fluorescence-coupled bioreporters. Inside microcolonies, interactions between nearby cells are likely and may cause interference during perturbation studies. In this paper, we present a microfluidic device containing hundred sub-micron sized trapping barrier structures for single E. coli cells. Descendant cells are rapidly washed away as well as components secreted by growing cells. Experiments show excellent growth rates, indicating high cell viability. Analyses of elongation and growth rates as well as morphology were successfully performed. This device will find application in prokaryotic single-cell studies under constant environment where by-product interference is undesired

EU Soil Strategy Actions Tracker

The EU Soil Strategy for 2030 sets out a framework to ensure that soils are used sustainably by setting concrete measures to protect and restore soils. It sets a vision and objectives to achieve healthy soil by 2050, with concrete actions due by 2030. The newly developed ‘EU Soil Strategy Actions Tracker’ is a tool to track the progress of these concrete actions since the adoption of the EU Soil Strategy in 2021. The EU Soil Strategy Actions Tracker provides an overview on the status of the actions, information on the due date, recent updates, links to related EU policies, links to relevant sources, and the main actors responsible for implementation. Currently, 62 out of 90 actions (69%) are marked as completed, 24 actions (27%) as in progress, and 4 actions (4%) as withdrawn. The EU Soil Strategy Actions Tracker provides a clear overview of the progress made by the European Commission under the EU Soil Strategy. With almost 70% of complete actions, the tracker shows that the Commission is well on track to complete most of the actions included in the EU Soil Strategy. Together with the EU Soil Observatory (EUSO) Soil Degradation Dashboard, it provides an informative overview on EU soil health status and ongoing actions to preserve and restore the state of soils in the EU. The EU Soil Strategy Actions Tracker is the first step towards an EUSO Soil Policy Actions Tracker to monitor EU policy actions relevant to soils, other policies (e.g. EU Climate Law) are foreseen to be included in 2025.JRC.D.3 - Land Resources and Supply Chain Assessment