On possibility of measurement of the electron beam energy using absorption of radiation by electrons in a magnetic field

The possibility of the precise measurement of the electron beam energy using absorption of radiation by electrons in a static and homogeneous magnetic field in a range up to a few hundred GeV energies, was considered in [1]. With the purpose of experimental checking of this method in a range of several tens MeV energies, the possibility of measurement of absolute energy of the electron beam energy with relative accuracy up to 10^{-4} is examined in details.Comment: 14 pages, 10 figure

Application of Computing Technologies in Studying the Course «Building Materials»

Секция V : Проблемы инженерного образования в условиях меняющегося мира, его устойчивого развитияУсловия меняющегося мира предполагают переход высшего образования на новый этап развития с всё большим вовлечением в процесс обучения информационно-компьютерных технологий.=Сonditions of the varying world suppose a new stage of the development in higher education with using information computing technologies

Regulation of ABCC6 trafficking and stability by a conserved C-terminal PDZ-like sequence

Mutations in the ABCC6 ABC-transporter are causative of pseudoxanthoma elasticum (PXE). The loss of functional ABCC6 protein in the basolateral membrane of the kidney and liver is putatively associated with altered secretion of a circulatory factor. As a result, systemic changes in elastic tissues are caused by progressive mineralization and degradation of elastic fibers. Premature arteriosclerosis, loss of skin and vascular tone, and a progressive loss of vision result from this ectopic mineralization. However, the identity of the circulatory factor and the specific role of ABCC6 in disease pathophysiology are not known. Though recessive loss-of-function alleles are associated with alterations in ABCC6 expression and function, the molecular pathologies associated with the majority of PXE-causing mutations are also not known. Sequence analysis of orthologous ABCC6 proteins indicates the C-terminal sequences are highly conserved and share high similarity to the PDZ sequences found in other ABCC subfamily members. Genetic testing of PXE patients suggests that at least one disease-causing mutation is located in a PDZ-like sequence at the extreme C-terminus of the ABCC6 protein. To evaluate the role of this C-terminal sequence in the biosynthesis and trafficking of ABCC6, a series of mutations were utilized to probe changes in ABCC6 biosynthesis, membrane stability and turnover. Removal of this PDZ-like sequence resulted in decreased steady-state ABCC6 levels, decreased cell surface expression and stability, and mislocalization of the ABCC6 protein in polarized cells. These data suggest that the conserved, PDZ-like sequence promotes the proper biosynthesis and trafficking of the ABCC6 protein. © 2014 Xue et al

ИССЛЕДОВАНИЕ ОСОБЕННОСТЕЙ ФОРМИРОВАНИЯ БОРИДНЫХ ПОКРЫТИЙ НА КАРБОНИТРИРОВАННОЙ МАЛОУГЛЕРОДИСТОЙ СТАЛИ

The paper contains description about obtaining two-phase boride coatings on preliminary carbonitrated steel 20. The influence of a preliminary carbonitration on phase – and structure formation and properties of the boride coatings has been investigated in the paper.Получены двухфазные боридные покрытия на предварительно карбонитрированной стали 20. Исследовано влияние предварительной карбонитрации на фазо- и структурообразование и свойства боридных покрытий

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-dependent drug efflux pump involved in multidrug resistance of cancers.Codon-optimized "Opti-Pgp" and wild-type Pgp, identical in primary protein sequence, were rigorously analyzed for differences in function or solution structure. Yeast expression levels and yield of purified protein from P. pastoris (∼130 mg per kg cells) were about three-fold higher for Opti-Pgp than for wild-type protein. Opti-Pgp conveyed full in vivo drug resistance against multiple anticancer and fungicidal drugs. ATP hydrolysis by purified Opti-Pgp was strongly stimulated ∼15-fold by verapamil and inhibited by cyclosporine A with binding constants of 4.2±2.2 µM and 1.1±0.26 µM, indistinguishable from wild-type Pgp. Maximum turnover number was 2.1±0.28 µmol/min/mg and was enhanced by 1.2-fold over wild-type Pgp, likely due to higher purity of Opti-Pgp preparations. Analysis of purified wild-type and Opti-Pgp by CD, DSC and limited proteolysis suggested similar secondary and ternary structure. Addition of lipid increased the thermal stability from T(m) ∼40 °C to 49 °C, and the total unfolding enthalpy. The increase in folded state may account for the increase in drug-stimulated ATPase activity seen in presence of lipids.The significantly higher yields of protein in the native folded state, higher purity and improved function establish the value of our gene optimization approach, and provide a basis to improve production of other membrane proteins

ИССЛЕДОВАНИЕ ИЗНОСОСТОЙКОСТИ КАРБИДНЫХ И КАРБОНИТРИДНЫХ ПОКРЫТИЙ НА ЛЕГИРОВАННЫХ СТАЛЯХ

The paper describes investigations and comparative analysis pertaining to wear-resistance of carbide and carbonitride coatings which have been obtained on alloy steel from powder media while using conventional furnace heating and in the fluidized bed. Improvement of wear-resistance  has been obtained from 2 to 30 % during treatment process in the fluidized bed.Проведены исследования и сравнительный анализ износостойкости карбидных и карбонитридных покрытий, полученных на легированных сталях из порошковых сред при традиционном печном нагреве и в кипящем слое. Установлено повышение износостойкости от 2 до 30 % при обработке в кипящем слое.

РАЗРАБОТКА ПОРОШКОВЫХ СМЕСЕЙ ДЛЯ ПРОЦЕССА ОДНОФАЗНОГО БОРИРОВАНИЯ И ИССЛЕ- ДОВАНИЕ СВОЙСТВ ОДНОФАЗНЫХ БОРИДНЫХ СЛОЕВ

The paper presents results of the investigations in respect of powder media for realization of mono-phase borating process.Structure, chemical and phase composition of powder mixture are determined and properties of mono-phase boride coatings are investigated in the paper.Представлены результаты исследования порошковой среды для осуществления процесса однофазного борирования.Определены строение, химический и фазовый состав порошковой смеси и исследованы свойства однофазных боридных покрытий

Comparative study of thermostability and structure of close homologues - bamase and binase

Parameters of heat denaturation and intrinsic fluorescence of bamase and its close homologue, binase in the pH region 2-6 have been determined. The bamase heat denaturation (pH 2.85.5) proceeds according to the “all-or-none” principle. Bamase denaturation temperature is lower than that of binase and this difference increases from 2.5 °C at pH 5 to 7 °C at pH 3. Enthalpy values of bamase and binase denaturation coincide only at pH 4.5-5.5, but as far as pH decreases the bamase denaturation enthalpy decreases significantly and in this respect it differs from binase. The fluorescence and CD techniques do not reveal any distinctions in the local environment of aromatic residues in the two proteins, and the obtained difference in the parameters of intrinsic fluorescence is due to fluorescence quenching of the bamase Trp94 by the His 18 residue, absent in binase. Secondary structures of both native and denaturated proteins also do not differ. Some differences in the barnase and binase electrostatic characteristics, revealed in the character of the dipole moments distribution, have been found. © 1993 Taylor & Francis Ltd

БОРИРОВАНИЕ УГЛЕРОДИСТЫХ И ЛЕГИРОВАННЫХ СТАЛЕЙ В КИПЯЩЕМ СЛОЕ

The paper describes how to obtain boride coatings on steel 20, 4X5MФС, X12M being treated in a boiling layer of metallothermic powder environment. Phase and chemical compositions, hardness and wear- resistance of boride coatings Получены боридные покрытия на сталях 20, 4Х5МФС, Х12М, обработанных в кипящем слое из металлотермической порошковой среды. Исследованы фазовый, химический составы, твердость и износостойкость боридных покрытий.