Valori del diritto del lavoro ed economia di mercato = Values of labor law and market economy. WP C.S.D.L.E. “Massimo D’Antona”.IT – 395/2019

The author deals with the topic of labour law’s inspiring values, which are discussed in their connection and co-evolution with the processes of transformation of the economic and social reality. Under this perspective of analysis, the author firstly takes a stand on the controversial relationship between labour law and the economic rationality in a broad sense. The presence of two basic approaches within the labour law culture, which are characterised by divergent views and attitudes on the market economy, is maintained (§ 2). The ways in which the discipline has responded to the neoliberal attack is then discussed (§ 3). Against this background, some arguments in favour of an “economically sustainable” labour law, which is aimed at reaching the best possible compromise between social and economic instances, along the thread of the enhancement of human labour, are put forward (§ 4). On the basis of the described context, the author comes back to the topic of labour law’s values, in order to suggest a constructive reinterpretation of the latter, which makes them more fit to the current processes of transformation, in particular as regards the non-paternalistic promotion of the worker’s autonomy and role. The Capabilities Approach is proposed as an ideal guide in view of such a revisiting (§ 5). The article is concluded by basic remarks about the implications of the suggested approach as regards the selection of the regulation techniques and the impact evaluations (§ 6)

I diritti fondamentali e la trasformazione del diritto del lavoro = Fundamental rights and the transformation of labor law. WP C.S.D.L.E. “Massimo D’Antona”.IT – 333/2017

The author deals with the role of fundamental rights in the complex process of transformation of labour law. The peculiar way in which the topic of fundamental rights has entered into the labour law discourse is firstly examined: while private law has recently “discovered” the fundamental rights mainly in connection with the adoption of the European Charter of Fundamental Rights, this cannot be said of labour law, whose encounter with fundamental rights is much earlier, as it dates back to the process of interpretation and implementation of the Italian Constitution. In a sense, labour law didn’t need to theorize the fundamental rights as it was used to conceive itself as fundamental as a whole. Labour lawyers have later addressed the issue in a different perspective when that represented by fundamental rights has become a frontline in the battle to defend labour law from the challenges issued by global economy, mainly in terms of flexibility. In any case, the author doubts that the Constitution can represent a decisive obstacle to the adoption of liberalization labour policies, at least as soon as certain limits won’t overcome (but many labour lawyers think that they have been). Afterwards the article focuses on the European Charter of Fundamental Rights, arguing that despite its great importance for the edification of a socially-oriented European Union, it has not added very much, up to now and with the albeit relevant exception of dismissal, to the number and extension of fundamental rights as resulting from the Italian Constitutional order. In any case, the permanent relevance of the topic, also in view of further advancements, is finally underlined

Structure and sequence analyses of Bacteroides proteins BVU_4064 and BF1687 reveal presence of two novel predominantly-beta domains, predicted to be involved in lipid and cell surface interactions.

BackgroundN-terminal domains of BVU_4064 and BF1687 proteins from Bacteroides vulgatus and Bacteroides fragilis respectively are members of the Pfam family PF12985 (DUF3869). Proteins containing a domain from this family can be found in most Bacteroides species and, in large numbers, in all human gut microbiome samples. Both BVU_4064 and BF1687 proteins have a consensus lipobox motif implying they are anchored to the membrane, but their functions are otherwise unknown. The C-terminal half of BVU_4064 is assigned to protein family PF12986 (DUF3870); the equivalent part of BF1687 was unclassified.ResultsCrystal structures of both BVU_4064 and BF1687 proteins, solved at the JCSG center, show strikingly similar three-dimensional structures. The main difference between the two is that the two domains in the BVU_4064 protein are connected by a short linker, as opposed to a longer insertion made of 4 helices placed linearly along with a strand that is added to the C-terminal domain in the BF1687 protein. The N-terminal domain in both proteins, corresponding to the PF12985 (DUF3869) domain is a β-sandwich with pre-albumin-like fold, found in many proteins belonging to the Transthyretin clan of Pfam. The structures of C-terminal domains of both proteins, corresponding to the PF12986 (DUF3870) domain in BVU_4064 protein and an unclassified domain in the BF1687 protein, show significant structural similarity to bacterial pore-forming toxins. A helix in this domain is in an analogous position to a loop connecting the second and third strands in the toxin structures, where this loop is implicated to play a role in the toxin insertion into the host cell membrane. The same helix also points to the groove between the N- and C-terminal domains that are loosely held together by hydrophobic and hydrogen bond interactions. The presence of several conserved residues in this region together with these structural determinants could make it a functionally important region in these proteins.ConclusionsStructural analysis of BVU_4064 and BF1687 points to possible roles in mediating multiple interactions on the cell-surface/extracellular matrix. In particular the N-terminal domain could be involved in adhesive interactions, the C-terminal domain and the inter-domain groove in lipid or carbohydrate interactions

Complete genome sequences of Vibrio cholera: specific bacteriophages 24 and X29

The complete genomes of two Vibrio cholerae bacteriophages of potential interest for cholera bacteriophage (phage) therapy were sequenced and annotated. The genome size of phage 24 is 44,395 bp encoding 71 putative proteins, and that of phage X29 is 41,569 bp encoding 68 putative proteins

Decreto Legislativo 81/2008. Quale prevenzione nei luoghi di lavoro?

A contribution in the medical field illustrates the historical evolution of the concepts of prevention and health to which it is necessary to refer for a discussion about health and safety in the workplace. Some contributions in the fields of labor law and organizational theory interpret the norms currently effective in Italy and the guidelines for the assessment of risks in work situations. What conception of prevention is implicitly assumed by the norms, and by the guidelines that the norms indicate? Are the current norms adequate to stimulate organizational choices aimed at preventing risks and discouraging harmful choices? Limits and contradictions hindering a real, effective, primary prevention are emphasized

PDBTM: Protein Data Bank of transmembrane proteins after 8 years

The PDBTM database (available at http://pdbtm .enzim.hu), the first comprehensive and up-to-date transmembrane protein selection of the Protein Data Bank, was launched in 2004. The database was created and has been continuously updated by the TMDET algorithm that is able to distinguish between transmembrane and non-transmembrane proteins using their 3D atomic coordinates only. The TMDET algorithm can locate the spatial positions of transmembrane proteins in lipid bilayer as well. During the last 8 years not only the size of the PDBTM database has been steadily growing from ~400 to 1700 entries but also new structural elements have been identified, in addition to the well-known a-helical bundle and b-barrel structures. Numerous ‘exotic’ transmembrane protein structures have been solved since the first release, which has made it necessary to define these new structural elements, such as membrane loops or interfacial helices in the database. This article reports the new features of the PDBTM database that have been added since its first release, and our current efforts to keep the database up-to-date and easy to use so that it may continue to serve as a fundamental resource for the scientific community

LUD, a new protein domain associated with lactate utilization.

BackgroundA novel highly conserved protein domain, DUF162 [Pfam: PF02589], can be mapped to two proteins: LutB and LutC. Both proteins are encoded by a highly conserved LutABC operon, which has been implicated in lactate utilization in bacteria. Based on our analysis of its sequence, structure, and recent experimental evidence reported by other groups, we hereby redefine DUF162 as the LUD domain family.ResultsJCSG solved the first crystal structure [PDB:2G40] from the LUD domain family: LutC protein, encoded by ORF DR_1909, of Deinococcus radiodurans. LutC shares features with domains in the functionally diverse ISOCOT superfamily. We have observed that the LUD domain has an increased abundance in the human gut microbiome.ConclusionsWe propose a model for the substrate and cofactor binding and regulation in LUD domain. The significance of LUD-containing proteins in the human gut microbiome, and the implication of lactate metabolism in the radiation-resistance of Deinococcus radiodurans are discussed

Combining Raman and infrared spectroscopy as a powerful tool for the structural elucidation of cyclodextrin-based polymeric hydrogels

A detailed experimental and theoretical vibrational analysis of hydrogels of b-cyclodextrin nanosponges (b-CDNS), obtained by polymerization of b-cyclodextrin (b-CD) with the cross-linking agent ethylenediaminetetraacetic acid (EDTA), is reported here. Thorough structural characterization is achieved by exploiting the complementary selection rules of FTIR-ATR and Raman spectroscopies and by supporting the spectral assignments by DFT calculations of the spectral profiles. The combined analysis of the FTIR-ATR spectra of the polymers hydrated with H2O and D2O allowed us to isolate the HOH bending of water molecules not involved in symmetrical, tetrahedral environments. The analysis of the HOH bending mode was carried out as a function of temperature, showing the existence of a supercooled state of the water molecules. The highest level of cooperativity of the hydrogen bond scheme was reached at a value of the b-CD/EDTA molar ratio n = 6. Finally, the connectivity pattern of ‘‘uncoupled’’ water molecules bound to the nanosponge backbone was found to be weakened by increasing T. The temperature above which the population of non-tetracoordinated water molecules becomes predominant turned out to be independent of the parameter n

Transport properties of ibuprofen encapsulated in cyclodextrin nanosponge hydrogels: A proton HR-MAS NMR spectroscopy study

The chemical cross-linking of β-cyclodextrin (β-CD) with ethylenediaminetetraacetic dianhydride (EDTA) led to branched polymers referred to as cyclodextrin nanosponges (CDNSEDTA). Two different preparations are described with 1:4 and 1:8 CD-EDTA molar ratios. The corresponding cross-linked polymers were contacted with 0.27 M aqueous solution of ibuprofen sodium salt (IP) leading to homogeneous, colorless, drug loaded hydrogels. The systems were characterized by high resolution magic angle spinning (HR-MAS) NMR spectroscopy. Pulsed field gradient spin echo (PGSE) NMR spectroscopy was used to determine the mean square displacement (MSD) of IP inside the polymeric gel at different observation times td. The data were further processed in order to study the time dependence of MSD: MSD = f(td). The proposed methodology is useful to characterize the different diffusion regimes that, in principle, the solute may experience inside the hydrogel, namely normal or anomalous diffusion. The full protocols including the polymer preparation and purification, the obtainment of drug-loaded hydrogels, the NMR sample preparation, the measurement of MSD by HR-MAS NMR spectroscopy and the final data processing to achieve the time dependence of MSD are here reported and discussed. The presented experiments represent a paradigmatic case and the data are discussed in terms of innovative approach to the characterization of the transport properties of an encapsulated guest within a polymeric host of potential application for drug delivery