Spatio-Temporal Characteristics of Global Warming in the Tibetan Plateau during the Last 50 Years Based on a Generalised Temperature Zone - Elevation Model

Temperature is one of the primary factors influencing the climate and ecosystem, and examining its change and fluctuation could elucidate the formation of novel climate patterns and trends. In this study, we constructed a generalised temperature zone elevation model (GTEM) to assess the trends of climate change and temporal-spatial differences in the Tibetan Plateau (TP) using the annual and monthly mean temperatures from 1961-2010 at 144 meteorological stations in and near the TP. The results showed the following: (1) The TP has undergone robust warming over the study period, and the warming rate was 0.318°C/decade. The warming has accelerated during recent decades, especially in the last 20 years, and the warming has been most significant in the winter months, followed by the spring, autumn and summer seasons. (2) Spatially, the zones that became significantly smaller were the temperature zones of -6°C and -4°C, and these have decreased 499.44 and 454.26 thousand sq km from 1961 to 2010 at average rates of 25.1% and 11.7%, respectively, over every 5-year interval. These quickly shrinking zones were located in the northwestern and central TP. (3) The elevation dependency of climate warming existed in the TP during 1961-2010, but this tendency has gradually been weakening due to more rapid warming at lower elevations than in the middle and upper elevations of the TP during 1991-2010. The higher regions and some low altitude valleys of the TP were the most significantly warming regions under the same categorizing criteria. Experimental evidence shows that the GTEM is an effective method to analyse climate changes in high altitude mountainous regions

Identification and characterization of common carp (Cyprinus carpio L.) granzyme A/K, a cytotoxic cell granule-associated serine protease

Granzyme (Gzm) is an important member of serine protease family, and key component in the specific and non-specific cell-mediated cytotoxicity Partial GzmA/K cDNA sequence of common carp (Cyprinus carpi L) was isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently, the full length cDNA of carp GzmA/K was obtained by means of 3' RACE and 5' RACE, respectively The full length cDNA of carp GzmA/K was 1053 bp, consisting of a 5'-terminal untranslated region (UTR) of 65 bp, a 3'-terminal UTR of 214 bp, and an open reading frame of 774 bp Amino acid sequence analysis indicated the existence of a signal peptide, eight consensus cysteine residues, one conserved IIGG motif and three conserved residues as central elements of the GzmA/K active site. Carp GzmA/K shared 36% and 39% amino acid identity to human GzmA and K, respectively, and was phylogenetically related to the granzyme A and K subgroups Then, a genomic DNA, which covers the promoter region and entire coding region of carp GzmA/K, was obtained by PCR. In the 5.4 k-long genomic sequence, five exons and four introns were identified. Real-time RT-PCR analysis showed that carp GzmA/K transcript was predominantly detected in the immune-related tissues, and after SVCV infection, was up-regulated in most immune-related tissues in a time-dependent manner Real-time RT-PCR results also showed that carp GzmA/K transcript was up-regulated in thymus tissue of GH transgenic carp These results will help to understand the molecular characterization and the potential role of teleost GzmA/K, a cytotoxic cell granule-associated serine protease Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserve

The intersection form of production possibility set in DEA and its applications

2006-2007 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Microfluidic Perfusion for Regulating Diffusible Signaling in Stem Cells

Background Autocrine & paracrine signaling are widespread both in vivo and in vitro, and are particularly important in embryonic stem cell (ESC) pluripotency and lineage commitment. Although autocrine signaling via fibroblast growth factor-4 (FGF4) is known to be required in mouse ESC (mESC) neuroectodermal specification, the question of whether FGF4 autocrine signaling is sufficient, or whether other soluble ligands are also involved in fate specification, is unknown. The spatially confined and closed-loop nature of diffusible signaling makes its experimental control challenging; current experimental approaches typically require prior knowledge of the factor/receptor in order to modulate the loop. A new approach explored in this work is to leverage transport phenomena at cellular resolution to downregulate overall diffusible signaling through the physical removal of cell-secreted ligands. Methodology/Principal Findings We develop a multiplex microfluidic platform to continuously remove cell-secreted (autocrine\paracrine) factors to downregulate diffusible signaling. By comparing cell growth and differentiation in side-by-side chambers with or without added cell-secreted factors, we isolate the effects of diffusible signaling from artifacts such as shear, nutrient depletion, and microsystem effects, and find that cell-secreted growth factor(s) are required during neuroectodermal specification. Then we induce FGF4 signaling in minimal chemically defined medium (N2B27) and inhibit FGF signaling in fully supplemented differentiation medium with cell-secreted factors to determine that the non-FGF cell-secreted factors are required to promote growth of differentiating mESCs. Conclusions/Significance Our results demonstrate for the first time that flow can downregulate autocrine\paracrine signaling and examine sufficiency of extracellular factors. We show that autocrine\paracrine signaling drives neuroectodermal commitment of mESCs through both FGF4-dependent and -independent pathways. Overall, by uncovering autocrine\paracrine processes previously hidden in conventional culture systems, our results establish microfluidic perfusion as a technique to study and manipulate diffusible signaling in cell systems.National Institutes of Health (U.S.) (NIH grant No. EB007278)Swiss National Science FoundationSwiss National Science Foundatio

Synchronization Control for a Class of Discrete-Time Dynamical Networks With Packet Dropouts: A Coding-Decoding-Based Approach

10.13039/501100000288-Royal Society; 10.13039/501100001809-National Natural Science Foundation of China; 10.13039/501100000923-Australian Research Council; Program for Capability Construction of Shanghai Provincial Universities; 10.13039/100005156-Alexander von Humboldt-Stiftung

Determination of the number of J/ψ events with J/ψ → inclusive decays

postprin

Higher-order multipole amplitude measurement in ψ ′→γχ c2

Using 106×106 ψ ′ events collected with the BESIII detector at the BEPCII storage ring, the higher-order multipole amplitudes in the radiative transition ψ ′→γχ c2→γπ +π -/γK +K - are measured. A fit to the χ c2 production and decay angular distributions yields M2=0.046±0. 010±0.013 and E3=0.015±0.008±0.018, where the first errors are statistical and the second systematic. Here M2 denotes the normalized magnetic quadrupole amplitude and E3 the normalized electric octupole amplitude. This measurement shows evidence for the existence of the M2 signal with 4.4σ statistical significance and is consistent with the charm quark having no anomalous magnetic moment. © 2011 American Physical Society.published_or_final_versio

Controlling spin-dependent tunneling by bandgap tuning in epitaxial rocksalt MgZnO films

Widespread application of magnetic tunnel junctions (MTJs) for information storage has so far been limited by the complicated interplay between tunnel magnetoresistance (TMR) ratio and the product of resistance and junction area (RA). An intricate connection exists between TMR ratio, RA value and the bandgap and crystal structure of the barrier, a connection that must be unravelled to optimise device performance and enable further applications to be developed. Here, we demonstrate a novel method to tailor the bandgap of an ultrathin, epitaxial Zn-doped MgO tunnel barrier with rocksalt structure. This structure is attractive due to its good Δ1 spin filtering effect and we show that MTJs based on tunable MgZnO barriers allow effective balancing of TMR ratio and RA value. In this way spin-dependent transport properties can be controlled, a key challenge for the development of spintronic devices

Home-Based Self-Management After Permanent Pacemaker Implantation: What Should Patients Know

Han Yan,1,* Yu Shuang Chen,2,* Yi Li,2 Guan-Xing Wei,2 Fang Ma,3 Qiu Lan Hu,4 Lan Ding,5 Wei Wei,6 Yan Li,7 Yang-Juan Bai2 1Intensive Care Unit of Cardiac Surgery Department, QingDao Municipal Hospital (Group), Qingdao, Shandong, People’s Republic of China; 2The First Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 3Nursing Department, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 4Geriatric Intensive Care Unit, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 5Outpatient Department, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 6Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 7Department of Obstetrics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yang-Juan Bai, The First Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, Kunming, Yunnan, 650032, People’s Republic of China, Tel +8613678757051, Email [email protected]: The purpose of this study is to systematically explore and summarize the best evidence of home-based self-management in patients with permanent pacemaker implantation, providing comprehensive references and guidance for improving the self-management ability of such patients.Methods: Using the “ 6S” model, a systematic literature search was conducted on home-based self-management in patients with permanent pacemaker implantation. The types of literature included best practice, clinical decision-making, guidelines, expert consensus, systematic review and evidence summary. According to the inclusion and exclusion criteria, two researchers screened the literature, evaluated the quality, extracted the data, synthesized the evidence.Data Sources: BMJ Best Practice, Up To Date, Joanna Briggs Institute (JBI), the World Health Organization (WHO), the Guidelines International Network (GIN), the National Institute for Health and Care Excellence (NICE), the Registered Nurses Association of Ontario (RNAO), the Scottish Intercollegiate Guidelines Network (SIGN), the American Heart Association (AHA), the Cochrane Library, PubMed, CINAHL, Web of Science, Medlive, CNKI Database, SinoMed (China Biomedical Literature Database), Wanfang Database and VIP Database. The retrieval period spanned from the establishment of these databases until January 31, 2025.Results: A total of 21 articles were included, 1 best practice, 9 clinical decision-making, 5 guidelines, 3 expert consensus, 1 systematic review, and 2 evidence summaries. Thirty-five pieces of the best evidence from 7 aspects were summarized, including complication management, electromagnetic interference management, sports rehabilitation management, operative limb management, postoperative follow-up management, role management, and emotional management.Conclusion: This review comprehensively summarized the best evidence home-based self-management in patients with permanent pacemaker implantation. These findings are scientific and referential, and provide evidence-based support for nursing and management of patients during home-based rehabilitation.Keywords: permanent pacemaker, self-management, best evidence, evidence-based nursin