Short-term memory and vocabulary development in children with Down syndrome and children with specific language impairment

A longitudinal comparison was made between development of verbal and visuo-spatial short-term memory and vocabulary in children with Down syndrome (DS), children with specific language impairment (SLI), and typically developing children as a control group. Participants were 12 children with DS (6 males, 6 females; mean chronological age 9y 9mo [SD 2.8 mo], range 8y 6mo to 11y 4mo); nine children with SLI (4 males, 5 females; mean chronological age 3y 9mo [SD 4.8mo], range 3y 3mo to 4y 5mo); and 12 typically developing children (5 males, 7 females; mean chronological age 4y 4mo [SD 3.9mo], range 3y 3mo to 4y 3mo). Participants were matched on mental age (mean mental age 4y 3mo). All participants completed verbal short-term memory, visuo-spatial short-term memory, and expressive and receptive vocabulary tasks on three occasions over 1 year. Similarities were seen in the clinical groups for verbal short-term memory. There was some evidence of difficulty in visuo-spatial short-term memory in the children with SLI relative to the other groups, but all three groups showed overlap in visuo-spatial short-term memory performance. At the final time-point vocabulary performance in the clinical groups was similar; the typically developing children showed higher vocabulary abilities than both clinical groups

Cognitive abilities in children with specific language impairment: consideration of visuo-spatial skills

Background: The study is concerned with the cognitive abilities of children with specific language impairment (SLI). Previous research has indicated that children with SLI demonstrate difficulties with certain cognitive tasks despite normal non‐verbal IQ scores. It has been suggested that a general processing limitation might account for the pattern of language and cognitive difficulties seen in children with SLI. The performances on a visuo‐spatial short‐term memory task and a visuo‐spatial processing task were considered in a group of young children with SLI. Verbal short‐term memory was also measured. Aims: To identify whether children with SLI demonstrate difficulties with visuo‐spatial memory as well as verbal short‐term memory. To see whether a visuo‐spatial processing task without short‐term memory requirements is problematic for children with SLI. To consider performance on these tasks over time. Methods & Procedures: Nine children with SLI (mean age 3;9 years at the study outset) and nine typically developing children (mean age 3;9 years at the study outset) were visited on three occasions over 1 year. Verbal short‐term memory, visuo‐spatial short‐term memory and visuo‐spatial processing tasks were administered to the children, and performance over time was compared between the two groups. Outcomes & Results: The children with SLI performed at a lower level than the typically developing children on the verbal short‐term memory task. Both groups showed similar development on the verbal short‐term memory task and the visuo‐spatial processing task over time. Only the visuo‐spatial short‐term memory task showed slower development over time in the children with SLI relative to the typically developing children. Conclusions: Children with SLI demonstrated slower development on a visuo‐spatial short‐term memory task relative to typically developing children of the same chronological age. This finding has implications for speech and language therapists and other professionals working with children with SLI. It may mean that only certain types of visual support are suitable, and that children with SLI will have difficulty with tasks requiring a high level of processing, or a number of mental manipulations

Organising undergraduate research projects: Student-led and academic-led models

Purpose: This paper addresses the management of undergraduate final year research dissertations. It intends to explain and clarify our experience of two models of delivery (student-led/academic-led) with reference to interest development theory (Hidi and Renninger, 2006). Approach: We focus on the advantages and drawbacks of each model within the context of the research literature, and describe a case study of the experiences of lecturers and students in one Division of a metropolitan UK University, running a leading programme in Speech & Language Therapy (Pathology). Recommendations are made which are intended to be of use to colleagues across disciplines and organisations. Findings: We argue that a delivery where students can choose their research topic from a limited set suggested by supervisors (academic-led model) is best placed to meet motivational challenges in Hidi and Renninger’s framework, and also increase feasibility for staff. We discuss how such a model might best be implemented. Originality: Describing case study experiences within a conceptual framework is important for the development of improved supervision methods. It is hoped that this case study paper will inform other institutions by providing clear theoretical underpinnings and practical recommendations; and that it will lead to further empirical research into models of organising final year dissertations

Aboveground Biomass Estimation for Three Common Woody Species in the Post Oak Savannah of Texas

The Post Oak Savannah occupies about 3.4 million hectares of gently rolling to hilly lands in east central Texas. Large post oak (Quercus stellata Wangenh.) blackjack oak (Quercus marilandica Munchh.), Eastern redcedar (Juniperus virginiana L.) and honey mesquite (Juniperus virginiana L.) usually form the overstory, often above thickets of yaupon (Ilex vomitoria), winged elm (Ulmus alata), gum bumelia (Sideroxylon lanuginosum Michx. Subsp. Oblongifolium (Nutt) T.D. Penn.), and live oak (Quercus virginiana Mill.). Historically limited to rocky hillsides and draws (Owens and Ansley 1997), these species have migrated over the last several hundred years into bottomlands where grasses once dominated, and the increase in abundance and range has fluctuated due to both the modification of the historic fire regime and overgrazing (Smeins and Fuhlendorf 1997). The primary focus of previous fire studies in the Post Oak Savannah have been ignition time, mortality rate and the effect of burning to the understory vegetation, not standing shrub biomass estimation. Biomass estimation equations developed in different regions may not be applicable to the Post Oak Savannah since these substitutions may result in substantial error (Grier and Milne 1981, Gottfried and Severson 1994). With better prediction equations for this region with an increasing Urban-Wildfire Interface, managers can more accurately estimate the potential severity of wildfires or the effects of prescribed burns (Martin et al. 1978). Biomass estimation methods that involve juniper species have focused on Pinyon-Juniper (Pinus edulis and Juniperus spp.) and overstory-understory interactions in the western states. Schnell (1976) developed biomass prediction equations tables for eastern redcedar in Georgia, Alabama Tennessee and Virginia, that required diameters at breast height (DBH) \u3e 12.7 cm. Clark et al. (1986) and Phillips (1981) developed equations for estimating post oak biomass in North Carolina, South Carolina and Georgia, using DBH and total height; Phillips (1981) also age, but neither included foliage. Common in both studies was a DBH \u3e 15.2 cm and total height as independent variables. There is little biomass estimation information available for gum bumelia, although Bryant and Kothmann (1979) suggested a quadratic equation might work best. The objective of this study was to develop regression models to predict the total above-ground biomass for three species commonly found in Post Oak Savannah plant communities

Narrative skills in deaf children who use spoken English: Dissociations between macro and microstructural devices

Previous research has highlighted that deaf children acquiring spoken English have difficulties in narrative development relative to their hearing peers both in terms of macro-structure and with micro-structural devices. The majority of previous research focused on narrative tasks designed for hearing children that depend on good receptive language skills. The current study compared narratives of 6 to 11-year-old deaf children who use spoken English (N=59) with matched for age and non-verbal intelligence hearing peers. To examine the role of general language abilities, single word vocabulary was also assessed. Narratives were elicited by the retelling of a story presented non-verbally in video format. Results showed that deaf and hearing children had equivalent macro-structure skills, but the deaf group showed poorer performance on micro-structural components. Furthermore, the deaf group gave less detailed responses to inferencing probe questions indicating poorer understanding of the story's underlying message. For deaf children, micro-level devices most strongly correlated with the vocabulary measure. These findings suggest that deaf children, despite spoken language delays, are able to convey the main elements of content and structure in narrative but have greater difficulty in using grammatical devices more dependent on finer linguistic and pragmatic skills

Contaminants in commercial preparations of ‘purified’ small leucine-rich proteoglycans may distort mechanistic studies

The authors are grateful to Genodisc (EC’s 7th Framework Programme (FP7, 2007-2013) under grant agreement no. HEALTH-F2-2008-201626) and the Orthopaedic Institute Ltd for funding.This paper reports the perplexing results that came about because of seriously impure commercially available reagents. Commercial reagents and chemicals are routinely ordered by scientists and are expected to have been rigorously assessed for their purity. Unfortunately, we found this assumption to be risky. Extensive work was carried out within our laboratory using commercially-sourced preparations of the small leucine-rich proteoglycans, decorin and biglycan, to investigate their influence on nerve cell growth. Unusual results compelled us to analyse the composition and purity of both preparations of these proteoglycans using both mass spectrometry and Western blotting, with and without various enzymatic deglycosylations. Commercial ‘decorin’ and ‘biglycan’ were found to contain a mixture of proteoglycans including not only both decorin and biglycan but also fibromodulin and aggrecan. The unexpected effects of ‘decorin’ and ‘biglycan’ on nerve cell growth could be explained by these impurities. Decorin and biglycan contain either chondroitin or dermatan sulphate glycosaminoglycan chains whilst fibromodulin only contains keratan sulphate and the large (>2,500 kDa), highly glycosylated aggrecan, contains both keratan and chondroitin sulphate. The different structure, molecular weights and composition of these impurities significantly affected our work and any conclusions that could be made. These findings beg the question as to whether scientists need to verify the purity of each commercially obtained reagent used in their experiments. The implications of these findings are vast, since the effects of these impurities may already have led to inaccurate conclusions and reports in the literature with concomitant loss of researchers’ funds and time.Publisher PDFPeer reviewe

Understory vegetative Diversity of Post-Thinned pine Plantations Treated with Fertilizer, Fire and Herbicide in East Texas

This study assessed biodiversity in the understory of two pine plantations where different management tools (fertilizer, prescribed burning, and herbicide application) were utilized. During three growing seasons, species, percent cover, and number of individuals, and physical characteristics were recorded. Responses to treatment were examined based on comparison of species richness, evenness, diversity, and importance. Two years after treatment, fertilized plots showed a decline in species richness, evenness, and diversity. Prescribed burning and herbicide treatments increased species richness but decreased species evenness, resulting in no change in diversity index. Herbicide treatment reduced the importance of dominant shrubs and increased the importance of disturbance-adapted species

Regulation of microRNA during cardiomyocyte maturation in sheep.

BACKGROUND: There is a limited capacity to repair damage in the mammalian heart after birth, which is primarily due to the inability of cardiomyocytes to proliferate after birth. This is in contrast to zebrafish and salamander, in which cardiomyocytes retain the ability to proliferate throughout life and can regenerate their heart after significant damage. Recent studies in zebrafish and rodents implicate microRNA (miRNA) in the regulation of genes responsible for cardiac cell cycle progression and regeneration, in particular, miR-133a, the miR-15 family, miR-199a and miR-590. However, the significance of these miRNA and miRNA in general in the regulation of cardiomyocyte proliferation in large mammals, including humans, where the timing of heart development relative to birth is very different than in rodents, is unclear. To determine the involvement of miRNA in the down-regulation of cardiomyocyte proliferation occurring before birth in large mammals, we investigated miRNA and target gene expression in sheep hearts before and after birth. The experimental approach included targeted transcriptional profiling of miRNA and target mRNA previously identified in rodent studies as well as genome-wide miRNA profiling using microarrays. RESULTS: The cardiac expression of miR-133a increased and its target gene IGF1R decreased with increasing age, reaching their respective maximum and minimum abundance when the majority of ovine cardiomyocytes were quiescent. The expression of the miR-15 family members was variable with age, however, four of their target genes decreased with age. These latter profiles are inconsistent with the direct involvement of this family of miRNA in cardiomyocyte quiescence in late gestation sheep. The expression patterns of 'pro-proliferative' miR-199a and miR-590 were also inconsistent with their involvement in cardiomyocyte quiescence. Consequently, miRNA microarray analysis was undertaken, which identified six discrete clusters of miRNA with characteristic developmental profiles. The functions of predicted target genes for the miRNA in four of the six clusters were enriched for aspects of cell division and regulation of cell proliferation suggesting a potential role of these miRNA in regulating cardiomyocyte proliferation. CONCLUSION: The results of this study show that the expression of miR-133a and one of its target genes is consistent with it being involved in the suppression of cardiomyocyte proliferation, which occurs across the last third of gestation in sheep. The expression patterns of the miR-15 family, miR-199a and miR-590 were inconsistent with direct involvement in the regulation cardiomyocyte proliferation in sheep, despite studies in rodents demonstrating that their manipulation can influence the degree of cardiomyocyte proliferation. miRNA microarray analysis suggests a coordinated and potentially more complex role of multiple miRNA in the regulation of cardiomyocyte quiescence and highlights significant differences between species that may reflect their substantial differences in the timing of this developmental process

Semantic fluency in deaf children who use spoken and signed language, in comparison to hearing peers

Background Deafness has an adverse impact on children’s ability to acquire spoken languages. Signed languages offer a more accessible input for deaf children, but because the vast majority are born to hearing parents who do not sign, their early exposure to sign language is limited. Deaf children as a whole are therefore at high risk of language delays. Aims We compared deaf and hearing children’s performance on a semantic fluency task. Optimal performance on this task requires a systematic search of the mental lexicon, the retrieval of words within a subcategory, and, when that subcategory is exhausted, switching to a new subcategory. We compared retrieval patterns between groups, and also compared the responses of deaf children who used British Sign Language (BSL) to those who used spoken English. We investigated how semantic fluency performance related to children’s expressive vocabulary and executive function skills, and also re-tested semantic fluency in the majority of the children nearly two years later, in order to investigate how much progress they had made in that time. Methods and procedures Participants were deaf children aged 6-11 years (N=106, comprising 69 users of spoken English, 29 users of BSL and 8 users of Sign Supported English) compared to hearing children (N=120) of the same age who used spoken English. Semantic fluency was tested for the category “animals”. We coded for errors, clusters (e.g., “pets”, “farm animals”) and switches. Participants also completed the Expressive One-Word Picture Vocabulary Test and a battery of six non-verbal executive function tasks. In addition, we collected follow-up semantic fluency data for 70 deaf and 74 hearing children, nearly 2 years after they were first tested. Outcomes and results Deaf children, whether using spoken or signed language, produced fewer items in the semantic fluency task than hearing children, but they showed similar patterns of responses for items most commonly produced, clustering of items into subcategories and switching between subcategories. Both vocabulary and executive function scores predicted the number of correct items produced. Follow-up data from deaf participants showed continuing delays relative to hearing children two years later. Conclusions and implications We conclude that semantic fluency can be used experimentally to investigate lexical organisation in deaf children, and that it potentially has clinical utility across the heterogeneous deaf population. We present normative data to aid clinicians who wish to use this task with deaf children

Associated reading skills in children with a history of Specific Language Impairment (SLI)

A large cohort of 200 eleven-year-old children with Specific Language Impairment (SLI) were assessed on basic reading accuracy and on reading comprehension as well as language tasks. Reading skills were examined descriptively and in relation to early language and literacy factors. Using stepwise regression analyses in which age and nonverbal IQ were controlled for, it was found that a single word reading measure taken at 7 years was unsurprisingly a strong predictor of the two different types of reading ability. However, even with this measure included, a receptive syntax task (TROG) entered when reading accuracy score was the DV. Furthermore, a test of expressive syntax/narrative and a receptive syntax task completed at 7 years entered into the model for word reading accuracy. When early reading accuracy was excluded from the analyses, early phonological skills also entered as a predictor of both reading accuracy and comprehension at 11 years. The group of children with a history of SLI were then divided into those with no literacy difficulties at 11 and those with some persisting literacy impairment. Using stepwise logistic regression, and again controlling for IQ and age, 7 years receptive syntax score (but not tests of phonology, expressive vocabulary or expressive syntax/narrative) entered as a positive predictor of membership of the ‘no literacy problems’ group regardless of whether early reading accuracy was controlled for in step one. The findings are discussed in relation to the overlap of SLI and dyslexia and the long term sequelae of language impairment