Development of novel plant-based adjuvant formulation against rubella and hepatitis B vaccine antigen

Introduction: Numerous metabolites present in the aqueous extract from plants are responsible for inducing adjuvant activity against rubella and hepatitis B vaccine antigen (HBsAg). One of the medicinal plants, Adhatoda vasica has been pointed out with great potential of vaccine adjuvant property

Inhibitory Potential of Aqueous Leaves Extract of Mesua Ferrea and Mimusops Elengi on Antigen Specific Immune Response using Human Whole Blood

ABSTRACT: The objective of this study is to examine the effect of variable doses of aqueous leaves extract of Mesua ferrea and Mimusops elengi (0.5 -10 mg/ml, 50 µl), medicinal plant on human lysed whole blood (cultured for 48 h) in order to determine the antigen (IBD, infectious bursal disease; virus derived from chicken) specific immune response including CD14 monocyte surface marker which is determined through flow cytometry. The results showed that Mesua ferrea and Mimusops elengi (10 mg/ml, 50 µl) showed dose dependent decline in antigen specific immune response including CD14 monocyte surface marker as compared to IBD virus and control. IBD used as standard for these studies and the results showed that there is significant enhancement in antigen specific immune response and CD14 monocyte surface marker as compared to control. Moreover, the outcomes of the work provide a platform on the way to discover novel immunosuppressive as well as anti-viral agents against IBD virus from plant origin

Anti-inflammatory and immunosuppressive activities of flavonoids from medicinal plants

Introduction: Medicinal plants have been used to treat various immunological diseases. Nitric oxide (NO) including proliferation and CD14 monocyte surface marker has played an important regulatory role in various types of inflammatory processes. This study was aimed to evaluate or scrutinize the effect of flavonoid extracted from the leaves of Santalum album, Butea frondosa and Emblica officinalis for determining their anti-inflammatory and immunosuppressive activity on human peripheral blood mononuclear cells (PBMC) using hepatitis B vaccine antigen (20 μg/mL; 10 μl). Methods: Flavonoids extracted from these medicinal plants were prepared (0.5–10 mg/mL; 50 μl) and their effects on human PBMC proliferation were examined using HBsAg. The NO production including CD14 monocyte surface marker was also estimated. Results: All these flavonoids at higher doses demonstrated a significant decrease in proliferation, NO production and CD14 surface marker. This inhibitory effect was seen after 48 hours of treatment. Conclusion: These results may indicate the presence of anti-inflammatory and immunosuppressive activity in these medicinal plants