An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans

The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating population studies and food genetic traceability has not been studied yet. The objective of the present work was to evaluate four methods of DNA extraction from leaves and chanar-based foods. The methods were evaluated based on yield, DNA purity, and molecular markers. The CCI-P (CTAB/Chloroform-Isoamylalcohol/pellet) method showed the highest yield of DNA obtained from leaves. However, the CPCI-SC (CTAB/Phenol-Chloroform-Isoamylalcohol/silica-column) method was the only one that resulted in acceptable DNA quality with both parameters (A260/A280 and A260/A230). The leaf DNA obtained with this method showed a greater amount of fragments with RAPD, and an acceptable amount of fragments with ISSR. On the other hand, the CCI-P method showed a higher yield of DNA from arrope de chanar (syrup). However, the CPCI-SC method was the only one that had relatively better DNA quality, which allowed the amplification of molecular markers. Regarding chanar flour, the CPCI-SC method showed the highest yield, DNA quality and good amplification with molecular markers. Therefore, the CPCI-SC extraction method is efficient for obtaining DNA from different matrices, and can support studies for a possible designation of origin of chanar-based foods

Holographic model for heavy vector meson masses

The experimentally observed spectra of heavy vector meson radial excitations show a dependence on two different energy parameters. One is associated with the quark mass and the other with the binding energy levels of the quark anti-quark pair. The first is present in the large mass of the first state while the other corresponds to the small mass splittings between radial excitations. In this article we show how to reproduce such a behavior with reasonable precision using a holographic model. In the dual picture, the large energy scale shows up from a bulk mass and the small scale comes from the position of anti-de Sitter (AdS) space where field correlators are calculated. The model determines the masses of four observed S-wave states of charmonium and six S-wave states of bottomonium with , 6.1 % rms error. In consistency with the physical picture, the large energy parameter is flavor dependent, while the small parameter, associated with quark anti-quark interaction is the same for charmonium and bottomonium states.Comment: In V5 we just added some clarifying explanations about the model. 5 tables, no figure. Version published in Europhysics Letter

Holographic Picture of Heavy Vector Meson Melting

The fraction of heavy vector mesons produced in a heavy ion collision, as compared to a proton proton collision, serves as an important indication of the formation of a thermal medium, the quark gluon plasma. This sort of analysis strongly depends on understanding the thermal effects of a medium like the plasma on the states of heavy mesons. In particular, it is crucial to know the temperature ranges where they undergo a thermal dissociation, or melting. AdS/QCD models are know to provide an important tool for the calculation of hadronic masses, but in general are not consistent with the observation that decay constants of heavy vector mesons decrease with excitation level. It has recently been shown that this problem can be overcome using a soft wall background and introducing an extra energy parameter, through the calculation of correlation functions at a finite position of anti-de Sitter space. This approach leads to the evaluation of masses and decay constants of S wave quarkonium states with just one flavor dependent and one flavor independent parameters. Here we extend this more realistic model to finite temperatures and analyse the thermal behavior of the states $1S, 2S$ and $3S$ of bottomonium and charmonium. The corresponding spectral function exhibits a consistent picture for the melting of the states where, for each flavor, the higher excitations melt at lower temperatures. We estimate for these six states, the energy ranges in which the heavy vector mesons undergo a transition from a well defined peak in the spectral function to complete melting in the thermal medium. A very clear distinction between the heavy flavors emerges, with bottomonium state $\Upsilon (1S)$ surviving deconfinemet transition at temperatures much larger than the critical deconfinement temperature of the medium.Comment: 20 pages, 7 figure

Non-equilibrium correlations and entanglement in a semiconductor hybrid circuit-QED system

We present a theoretical study of a hybrid circuit-QED system composed of two semiconducting charge-qubits confined in a microwave resonator. The qubits are defined in terms of the charge states of two spatially separated double quantum dots (DQDs) which are coupled to the same photon mode in the microwave resonator. We analyze a transport setup where each DQD is attached to electronic reservoirs and biased out-of-equilibrium by a large voltage, and study how electron transport across each DQD is modified by the coupling to the common resonator. In particular, we show that the inelastic current through each DQD reflects an indirect qubit-qubit interaction mediated by off-resonant photons in the microwave resonator. As a result of this interaction, both charge qubits stay entangled in the steady (dissipative) state. Finite shot noise cross-correlations between currents across distant DQDs are another manifestation of this nontrivial steady-state entanglement.Comment: Final versio

Ergodic Transport Theory, periodic maximizing probabilities and the twist condition

The present paper is a follow up of another one by A. O. Lopes, E. Oliveira and P. Thieullen which analyze ergodic transport problems. Our main focus will a more precise analysis of case where the maximizing probability is unique and is also a periodic orbit. Consider the shift T acting on the Bernoulli space \Sigma={1, 2, 3,.., d}^\mathbb{N} $and$A:\Sigma \to \mathbb{R} a Holder potential. Denote m(A)=max_{\nu is an invariant probability for T} \int A(x) \; d\nu(x) and, \mu_{\infty,A}, any probability which attains the maximum value. We assume this probability is unique (a generic property). We denote \T the bilateral shift. For a given potential Holder A:\Sigma \to \mathbb{R}, we say that a Holder continuous function W: \hat{\Sigma} \to \mathbb{R} is a involution kernel for A, if there is a Holder function A^*:\Sigma \to \mathbb{R}, such that, A^*(w)= A\circ \T^{-1}(w,x)+ W \circ \T^{-1}(w,x) - W(w,x). We say that A^* is a dual potential of A. It is true that m(A)=m(A^*). We denote by V the calibrated subaction for A, and, V^* the one for A^*. We denote by I^* the deviation function for the family of Gibbs states for \beta A, when \beta \to \infty. For each x we get one (more than one) w_x such attains the supremum above. That is, solutions of V(x) = W(w_x,x) - V^* (w_x)- I^*(w_x). A pair of the form (x,w_x) is called an optimal pair. If \T is the shift acting on (x,w) \in {1, 2, 3,.., d}^\mathbb{Z}, then, the image by \T^{-1} of an optimal pair is also an optimal pair. Theorem - Generically, in the set of Holder potentials A that satisfy (i) the twist condition, (ii) uniqueness of maximizing probability which is supported in a periodic orbit, the set of possible optimal w_x, when x covers the all range of possible elements x in \in \Sigma, is finite

Quantitative representation of reactivity, selectivity and site activation concepts in organic chemistry

Indexación: ScieloReactivity, selectivity and site activation are classical concepts in chemistry which are amenable to quantitative representation, in terms of static global, local and non local density response functions. The use of these electronic indexes describing chemical interconversion is developed in this work along the perspective of the pioneering work conducted in Chile by the late Professor Fernando Zuloaga, to whom this article is dedicated in memoriam. While global responses, represented as derivatives of the electronic energy with respect to the total number of electrons quantitatively describe the propensity of a system to interconvert into another chemical species (chemical reactivity), the local counterparts assesses well those regions in the molecule where the reactivity pattern dictated by the global quantities is developed (selectivity). Site activation /deactivation may in turn be described by the variations in the local or regional patterns of reactivity, that may be induced by solvent effects or chemical substitution. These concepts are illustrated for a series of chemical reactions in Organic Chemistry, including electrocyclic processes, cycloadditions and electrophilic addition reactions. Some relationships between quantitative scales of reactivity and reaction mechanisms are discussed.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072004000100010&lang=p