PRIMI DATI SUGLI INNESTI DI ABIES NEBRODENSIS (LOJAC.) MATTEI ESEGUITI SU ABETI ESOTICI NELL\u2019AREA DI INDIGENATO (MADONIE)

C2 = PRIMI DATI SUGLI INNESTI DI ABIES NEBRODENSIS (LOJAC.) MATTEI ESEGUITI SU ABETI ESOTICI NELL\u2019AREA DI INDIGENATO (MADONIE) R. SCHICCHI, P. BONOMO, M. CANDORE, G. DI NOTO, A. PUCCI, F. TRAPANI, P. MARINO Dipartimento di Biologia Ambientale e Biodiversit\ue0 dell'Universit\ue0, via Archirafi 38, 90123 Palermo (I) [email protected] Abies nebrodensis \ue8 una specie endemica relitta, gravemente minacciata di estinzione, la cui popolazione naturale \ue8 costituita da 30 individui distribuiti discontinuamente in una piccola area del territorio di Polizzi Generosa, all\u2019interno del Parco naturale delle Madonie in Sicilia. La minaccia di estinzione \ue8 dovuta, oltre all'esiguit\ue0 della popolazione, al ridotto numero di piante mature (ventiquattro) e al pericolo di inquinamento genetico incombente. A partire dall\u2019inizio degli anni \u201880 del secolo scorso, infatti, sono stati introdotti nella vicinanze dell\u2019area di indignato individui di abeti esotici (Abies alba Mill., A. cephalonica Loudon, e A. nordmanniana (Stefen) Spach) che, avendo raggiunto la maturit\ue0 sessuale, potrebbero mettere a rischio l\u2019integrit\ue0 genetica della progenie dell\u2019abete locale (Raimondo & Schicchi, 2005). Nell\u2019ambito del progetto \u201cConservazione di Abies nebrodensis e ripristino delle torbiere di Geraci Siculo\u201d, in corso di attuazione da parte dell\u2019Ente Parco delle Madonie, del Dipartimento di Biologia ambientale e Biodiversit\ue0 di Palermo e di altri partners locali, un\u2019azione di salvaguardia prevede l\u2019innesto degli abeti esotici con marze di A. nebrodensis, valorizzando la positiva esperienza effettuata con il progetto Life Natura (1). A tal fine, nel periodo compreso tra la terza decade di aprile e la seconda decade di maggio sono stati effettuati circa 350 innesti in contrada Comunello, nel territorio di Isnello. Le marze sono state prelevate da individui maturi di A. nebrodensis di oltre 30 anni di et\ue0, introdotti nelle opere di ripopolamento che, in seguito ai risultati delle analisi genetiche, sono geneticamente correlati agli esemplari della popolazione naturale. Gli innesti sono stati eseguiti a spacco ma soprattutto a corona, previa capitozzatura dei soggetti di A. alba e A. cephalonica, a un\u2019altezza di circa 2 m per evitare possibili danneggiamenti da parte del bestiame. Nei soggetti sono stati, comunque, rilasciati temporaneamente alcuni palchi che, continuando a svolgere le normali funzioni vitali, agevoleranno l\u2019attecchimento delle marze. Queste ultime sono state opportunamente sagomate e inserite tra la corteccia e il legno sia nel fusto capitozzato che in alcuni rami laterali. Esse sono state tenute strettamente aderenti ai soggetti tramite nastro adesivo e protette da due sacchetti, rispettivamente di polietilene e di carta, per creare condizioni micro-climatiche ottimali alla sopravvivenza delle marze. Dalla terza decade di maggio \ue8 iniziato, con cadenza settimanale, il monitoraggio degli innesti e la foratura, ove necessario, del sacchetto di plastica per eliminare l\u2019umidit\ue0 in eccesso. Dopo 40 giorni \ue8 stato possibile constatare che oltre il 90% degli innesti \ue8 attecchito. Anche alcuni innesti eseguiti a scopo sperimentale su Pseudotsuga menziesii Franco sono vitali. Durante la fine di luglio e agosto si prevede una graduale scopertura delle marze. Gli esiti di questa azione, tuttavia, potranno essere valutati compiutamente all\u2019inizio della prossima primavera. La tecnica dell\u2019innesto riveste notevole importanza poich\ue9 permette di eliminare o ridurre una delle minacce pi\uf9 consistenti a carico della popolazione naturale di A. nebrodensis, trasformando la stessa in un punto di forza nell\u2019ambito della strategia di conservazione della specie. Essa, infatti, potr\ue0 contribuire a incrementare la popolazione dell\u2019endemico A. nebrodensis, valorizzando come portainnesti piante che se non abbattute costituirebbero un serio pericolo per la conservazione dell\u2019abete delle Madonie. Raimondo F. M., Schicchi R., 2005. Rendiconto sul progetto LIFE Natura n\ub0 LIFE2000NAT/IT/7228 \u201cConservazione in situ ed ex situ di Abies nebrodensis (Lojac.) Mattei\u201d. Tipolitografia Luxograph, Palermo

An improved method on stimulated T-lymphocytes to functionally characterize novel and known LDLR mutations.

The main causes of familial hypercholesterolemia (FH) are mutations in LDL receptor (LDLR) gene. Functional studies are necessary to demonstrate the LDLR function impairment caused by mutations and would be useful as a diagnostic tool if they allow discrimination between FH patients and controls. In order to identify the best method to detect LDLR activity, we compared continuous Epstein-Barr virus (EBV)-transformed B-lymphocytes and mitogen stimulated T-lymphocytes. In addition, we characterized both novel and known mutations in the LDLR gene. T-lymphocytes and EBV-transformed B-lymphocytes were obtained from peripheral blood of 24 FH patients and 24 control subjects. Functional assays were performed by incubation with fluorescent LDL followed by flow cytometry analysis. Residual LDLR activity was calculated normalizing fluorescence for the mean fluorescence of controls. With stimulated T-lymphocytes we obtained a better discrimination capacity between controls and FH patients compared with EBV-transformed B-lymphocytes as demonstrated by receiver operating characteristic (ROC) curve analysis (the areas under the curve are 1.000 and 0.984 respectively; P < 0.0001 both). The characterization of LDLR activity through T-lymphocytes is more simple and faster than the use of EBV-transformed B-lymphocytes and allows a complete discrimination between controls and FH patients. Therefore the evaluation of residual LDLR activity could be helpful not only for mutation characterization but also for diagnostic purposes

Successful control of an outbreak of colonization by Klebsiella pneumoniae carbapenemase-producing K.\ua0pneumoniae sequence type 258 in a neonatal intensive care unit, Italy

This article reports an outbreak of colonization by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) sequence type (ST) 258 in a neonatal intensive care unit (NICU) in Palermo, Italy. KPC-Kp ST258 was detected by an active surveillance culture programme. Between 18th September and 14th November 2012, KPC-Kp was isolated from 10 out of 54 neonates admitted in the outbreak period. No cases of infection were recorded. Male sex was associated with colonization, whereas administration of ampicilline-sulbactam plus gentamicin was protective. Infection control interventions interrupted the spread of KPC-Kp without the need to close the NICU to new admissions. (C) 2013 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved

Flow cytometry analysis of acute promyelocytic leukemia: the power of 'surface hematology'

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Valorization of indigenous dairy cattle breed through salami production

The aim of the research was to produce salami manufactured with meat of three different commercial categories of bovine breed: cow on retirement, beef and young bull. A total of six experimental productions, at small-scale plant, were carried out with and without starter culture inoculums. The evolution of physico-chemical parameters in all trials followed the trend already registered for other fermented meat products. Several LAB species were found during process with different levels of species diversity and frequency of isolation among inoculated (mainly Pediococcus pentosaceus and Staphylococcus xylosus) and uninoculated (mainly Enterococcus devriesei, Lactobacillus curvatus and Lactobacillus sakei) trials. Enterobacteriaceae were found at very low levels during the entire ripening period and no pathogenic bacteria were found in any samples. The multivariate analysis showed that starter inoculums and meat affected significantly the physico-chemical and the microbiological composition of salami. The sensory analysis evidenced the highest overall acceptability was displayed by salami produced with meat from cow on retirement

THE ROLE OF BUTYRIC ACID AS A OPROTECTIVE AGENT AGAINST INFLAMMATORY BOWEL DISEASE

Inflammatory Bowel disease (IBD), such as Crohn's disease and ulcerative colitis, are pathologies characterized by a chronic inflammation of the gastrointestinal tract. Their etiopathogenesis is not yet fully understood. Immune system and heat shock proteins (HSPs) dysfunctions are considered to be among the most likely causes of these diseases. Butyrate is a short-chain fatty acid produced by intestinal microflora. It has a trophic, benefical and protective role in the colonic mucosa, and it also induces changes in Hsp levels and localization. It may therefore be a valuable complementary therapeutic agent when used alongside trraditional drugs (mesalazine and corticosteroids) to treat the production of butyrate in the endoluminal environment may promote clinical remission in IBD patients. Due to these characteristics, there has been keen interest in the use of butyrate as a novel therapeutic supplement in the recent years. The current findings need to be validated through further clinical trials to better define the bbiomolecular dynamics of butyrate in the colonocytes of IBD patients

Plant Conservation Centres in Sicily: The ‘Valle Maria’ Regional Centre (Godrano, Palermo) and the case study of the rare Centaurea erycina (Asteraceae).

The role of the ‘Valle Maria’ Regional Centre and its activity in plant conservation thank to the collaboration with the University of Palermo, Dept. STEBICEF is discussed. The case study on propagation of Centaurea erycina, a narrow Sicilian endemic is presented

Dark matter search in a Beam-Dump eXperiment (BDX) at Jefferson Lab

MeV-GeV dark matter (DM) is theoretically well motivated but remarkably unexplored. This Letter of Intent presents the MeV-GeV DM discovery potential for a 1 m$^3$ segmented plastic scintillator detector placed downstream of the beam-dump at one of the high intensity JLab experimental Halls, receiving up to 10$^{22}$ electrons-on-target (EOT) in a one-year period. This experiment (Beam-Dump eXperiment or BDX) is sensitive to DM-nucleon elastic scattering at the level of a thousand counts per year, with very low threshold recoil energies ($\sim$1 MeV), and limited only by reducible cosmogenic backgrounds. Sensitivity to DM-electron elastic scattering and/or inelastic DM would be below 10 counts per year after requiring all electromagnetic showers in the detector to exceed a few-hundred MeV, which dramatically reduces or altogether eliminates all backgrounds. Detailed Monte Carlo simulations are in progress to finalize the detector design and experimental set up. An existing 0.036 m$^3$ prototype based on the same technology will be used to validate simulations with background rate estimates, driving the necessary R$\&$D towards an optimized detector. The final detector design and experimental set up will be presented in a full proposal to be submitted to the next JLab PAC. A fully realized experiment would be sensitive to large regions of DM parameter space, exceeding the discovery potential of existing and planned experiments by two orders of magnitude in the MeV-GeV DM mass range.Comment: 28 pages, 17 figures, submitted to JLab PAC 4

Performance of prototypes for the ALICE electromagnetic calorimeter

The performance of prototypes for the ALICE electromagnetic sampling calorimeter has been studied in test beam measurements at FNAL and CERN. A $4\times4$ array of final design modules showed an energy resolution of about 11% /$\sqrt{E(\mathrm{GeV})}$ $\oplus$ 1.7 % with a uniformity of the response to electrons of 1% and a good linearity in the energy range from 10 to 100 GeV. The electromagnetic shower position resolution was found to be described by 1.5 mm $\oplus$ 5.3 mm /$\sqrt{E \mathrm{(GeV)}}$. For an electron identification efficiency of 90% a hadron rejection factor of $>600$ was obtained.Comment: 10 pages, 10 figure

The Main Results of the Borexino Experiment

The main physical results on the registration of solar neutrinos and the search for rare processes obtained by the Borexino collaboration to date are presented.Comment: 8 pages, 8 figgures, To be published as Proceedings of the Third Annual Large Hadron Collider Physics Conference, St. Petersburg, Russia, 201