Zukunftsfähige Stallkonzepte für die ökologische Milcherzeugung

Ensuring high animal welfare standards is one of the objectives of organic farming. The combination of modern housing concepts, proper herd management and good human-animal relationship could be an effective strategy. In this study 14 viable dairy barn concepts were described and evaluated. In nine of these dairy farms the management and animal welfare situation was examined. The outcome was a documentation of 14 different dairy farming concepts (housing, management) that are suitable to develop individual solutions with regard to animal welfare and the special requirements of organic dairy farms

Classification of graph C*-algebras with no more than four primitive ideals

We describe the status quo of the classification problem of graph C*-algebras with four primitive ideals or less

Bilinear modulation models for seasonal tables of counts

We propose generalized linear models for time or age-time tables of seasonal counts, with the goal of better understanding seasonal patterns in the data. The linear predictor contains a smooth component for the trend and the product of a smooth component (the modulation) and a periodic time series of arbitrary shape (the carrier wave). To model rates, a population offset is added. Two-dimensional trends and modulation are estimated using a tensor product B-spline basis of moderate dimension. Further smoothness is ensured using difference penalties on the rows and columns of the tensor product coefficients. The optimal penalty tuning parameters are chosen based on minimization of a quasi-information criterion. Computationally efficient estimation is achieved using array regression techniques, avoiding excessively large matrices. The model is applied to female death rate in the US due to cerebrovascular diseases and respiratory diseases

Disordered Topological Insulators via C∗C^*-Algebras

The theory of almost commuting matrices can be used to quantify topological obstructions to the existence of localized Wannier functions with time-reversal symmetry in systems with time-reversal symmetry and strong spin-orbit coupling. We present a numerical procedure that calculates a Z_2 invariant using these techniques, and apply it to a model of HgTe. This numerical procedure allows us to access sizes significantly larger than procedures based on studying twisted boundary conditions. Our numerical results indicate the existence of a metallic phase in the presence of scattering between up and down spin components, while there is a sharp transition when the system decouples into two copies of the quantum Hall effect. In addition to the Z_2 invariant calculation in the case when up and down components are coupled, we also present a simple method of evaluating the integer invariant in the quantum Hall case where they are decoupled.Comment: Added detail regarding the mapping of almost commuting unitary matrices to almost commuting Hermitian matrices that form an approximate representation of the sphere. 6 pages, 6 figure

Signatures of unresolved binaries in stellar spectra: implications for spectral fitting

The observable spectrum of an unresolved binary star system is a superposition of two single-star spectra. Even without a detectable velocity offset between the two stellar components, the combined spectrum of a binary system is in general different from that of either component, and fitting it with single-star models may yield inaccurate stellar parameters and abundances. We perform simple experiments with synthetic spectra to investigate the effect of unresolved main-sequence binaries on spectral fitting, modeling spectra similar to those collected by the APOGEE, GALAH, and LAMOST surveys. We find that fitting unresolved binaries with single-star models introduces systematic biases in the derived stellar parameters and abundances that are modest but certainly not negligible, with typical systematic errors of $300\,\rm K$ in $T_{\rm eff}$, 0.1 dex in $\log g$, and 0.1 dex in $[\rm Fe/H]$ for APOGEE-like spectra of solar-type stars. These biases are smaller for spectra at optical wavelengths than in the near-infrared. We show that biases can be corrected by fitting spectra with a binary model, which adds only two labels to the fit and includes single-star models as a special case. Our model provides a promising new method to constrain the Galactic binary population, including systems with single-epoch spectra and no detectable velocity offset between the two stars.Comment: Accept to MNRAS with minor revisions since v1. 7 pages, 5 figure

Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription.

Deregulated expression of MYC induces a dependence on the NUAK1 kinase, but the molecular mechanisms underlying this dependence have not been fully clarified. Here, we show that NUAK1 is a predominantly nuclear protein that associates with a network of nuclear protein phosphatase 1 (PP1) interactors and that PNUTS, a nuclear regulatory subunit of PP1, is phosphorylated by NUAK1. Both NUAK1 and PNUTS associate with the splicing machinery. Inhibition of NUAK1 abolishes chromatin association of PNUTS, reduces spliceosome activity, and suppresses nascent RNA synthesis. Activation of MYC does not bypass the requirement for NUAK1 for spliceosome activity but significantly attenuates transcription inhibition. Consequently, NUAK1 inhibition in MYC-transformed cells induces global accumulation of RNAPII both at the pause site and at the first exon-intron boundary but does not increase mRNA synthesis. We suggest that NUAK1 inhibition in the presence of deregulated MYC traps non-productive RNAPII because of the absence of correctly assembled spliceosomes

Evidence That the P\u3csub\u3ei\u3c/sub\u3e Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle

Nitrogenase reduction of dinitrogen (N2) to ammonia (NH3) involves a sequence of events that occur upon the transient association of the reduced Fe protein containing two ATP molecules with the MoFe protein that includes electron transfer, ATP hydrolysis, Pi release, and dissociation of the oxidized, ADP-containing Fe protein from the reduced MoFe protein. Numerous kinetic studies using the nonphysiological electron donor dithionite have suggested that the rate-limiting step in this reaction cycle is the dissociation of the Fe protein from the MoFe protein. Here, we have established the rate constants for each of the key steps in the catalytic cycle using the physiological reductant flavodoxin protein in its hydroquinone state. The findings indicate that with this reductant, the rate-limiting step in the reaction cycle is not protein–protein dissociation or reduction of the oxidized Fe protein, but rather events associated with the Pi release step. Further, it is demonstrated that (i) Fe protein transfers only one electron to MoFe protein in each Fe protein cycle coupled with hydrolysis of two ATP molecules, (ii) the oxidized Fe protein is not reduced when bound to MoFe protein, and (iii) the Fe protein interacts with flavodoxin using the same binding interface that is used with the MoFe protein. These findings allow a revision of the rate-limiting step in the nitrogenase Fe protein cycle

Biogenesis of mitochondrial porin

We review here the present knowledge about the pathway of import and assembly of porin into mitochondria and compare it to those of other mitochondrial proteins. Porin, like all outer mitochondrial membrane proteins studied so far is made as a precursor without a cleavble lsquosignalrsquo sequence; thus targeting information must reside in the mature sequence. At least part of this information appears to be located at the amino-terminal end of the molecule. Transport into mitochondria can occur post-translationally. In a first step, the porin precursor is specifically recognized on the mitochondrial surface by a protease sensitive receptor. In a second step, porin precursor inserts partially into the outer membrane. This step is mediated by a component of the import machinery common to the import pathways of precursor proteins destined for other mitochondrial subcompartments. Finally, porin is assembled to produce the functional oligomeric form of an integral membrane protein wich is characterized by its extreme protease resistance