Spatially Resolved Spectroscopy of Sub-AU-Sized Regions of T Tauri and Herbig Ae/Be Disks

We present spatially resolved near-IR spectroscopic observations of 15 young stars. Using a grism spectrometer behind the Keck Interferometer, we obtained an angular resolution of a few milli-arcseconds and a spectral resolution of 230, enabling probes of both gas and dust in the inner disks surrounding the target stars. We find that the angular size of the near-IR emission typically increases with wavelength, indicating hot, presumably gaseous material within the dust sublimation radius. Our data also clearly indicate Brackett-gamma emission arising from hot hydrogen gas, and suggest the presence of water vapor and carbon monoxide gas in the inner disks of several objects. This gaseous emission is more compact than the dust continuum emission in all cases. We construct simple physical models of the inner disk and fit them to our data to constrain the spatial distribution and temperature of dust and gas emission components.Comment: 40 pages, 8 figures. Accepted for publication in Ap

Spatially and Spectrally Resolved Hydrogen Gas within 0.1 AU of T Tauri and Herbig Ae/Be Stars

We present near-infrared observations of T Tauri and Herbig Ae/Be stars with a spatial resolution of a few milli-arcseconds and a spectral resolution of ~2000. Our observations spatially resolve gas and dust in the inner regions of protoplanetary disks, and spectrally resolve broad-linewidth emission from the Brackett gamma transition of hydrogen gas. We use the technique of spectro-astrometry to determine centroids of different velocity components of this gaseous emission at a precision orders of magnitude better than the angular resolution. In all sources, we find the gaseous emission to be more compact than or distributed on similar spatial scales to the dust emission. We attempt to fit the data with models including both dust and Brackett gamma-emitting gas, and we consider both disk and infall/outflow morphologies for the gaseous matter. In most cases where we can distinguish between these two models, the data show a preference for infall/outflow models. In all cases, our data appear consistent with the presence of some gas at stellocentric radii of ~0.01 AU. Our findings support the hypothesis that Brackett gamma emission generally traces magnetospherically driven accretion and/or outflows in young star/disk systems.Comment: 48 pages, including 17 figures. Accepted for publication by Ap

Stellar and Molecular Radii of a Mira Star: First Observations with the Keck Interferometer Grism

Using a new grism at the Keck Interferometer, we obtained spectrally dispersed (R ~ 230) interferometric measurements of the Mira star R Vir. These data show that the measured radius of the emission varies substantially from 2.0-2.4 microns. Simple models can reproduce these wavelength-dependent variations using extended molecular layers, which absorb stellar radiation and re-emit it at longer wavelengths. Because we observe spectral regions with and without substantial molecular opacity, we determine the stellar photospheric radius, uncontaminated by molecular emission. We infer that most of the molecular opacity arises at approximately twice the radius of the stellar photosphere.Comment: 12 pages, including 3 figures. Accepted by ApJ

First L-band Interferometric Observations of a Young Stellar Object: Probing the Circumstellar Environment of MWC 419

We present spatially-resolved K- and L-band spectra (at spectral resolution R = 230 and R = 60, respectively) of MWC 419, a Herbig Ae/Be star. The data were obtained simultaneously with a new configuration of the 85-m baseline Keck Interferometer. Our observations are sensitive to the radial distribution of temperature in the inner region of the disk of MWC 419. We fit the visibility data with both simple geometric and more physical disk models. The geometric models (uniform disk and Gaussian) show that the apparent size increases linearly with wavelength in the 2-4 microns wavelength region, suggesting that the disk is extended with a temperature gradient. A model having a power-law temperature gradient with radius simultaneously fits our interferometric measurements and the spectral energy distribution data from the literature. The slope of the power-law is close to that expected from an optically thick disk. Our spectrally dispersed interferometric measurements include the Br gamma emission line. The measured disk size at and around Br gamma suggests that emitting hydrogen gas is located inside (or within the inner regions) of the dust disk.Comment: Accepted for publication in Ap

Interferometric Observations of V838 Monocerotis

We have used long-baseline near-IR interferometry to resolve the peculiar eruptive variable V838 Mon and to provide the first direct measurement of its angular size. Assuming a uniform disk model for the emission we derive an apparent angular diameter at the time of observations (November-December 2004) of $1.83 \pm 0.06$ milli-arcseconds. For a nominal distance of $8\pm2$ kpc, this implies a linear radius of $1570 \pm 400 R_{\odot}$. However, the data are somewhat better fit by elliptical disk or binary component models, and we suggest that the emission may be strongly affected by ejecta from the outburst.Comment: 12 pages, 1 two-part encapsulated postscript figure. Accepted by ApJL. Added a table of observation

Science with the Keck Interferometer ASTRA Program

The ASTrometric and phase-Referenced Astronomy (ASTRA) project will provide phase referencing and astrometric observations at the Keck Interferometer, leading to enhanced sensitivity and the ability to monitor orbits at an accuracy level of 30-100 microarcseconds. Here we discuss recent scientific results from ASTRA, and describe new scientific programs that will begin in 2010-2011. We begin with results from the "self phase referencing" (SPR) mode of ASTRA, which uses continuum light to correct atmospheric phase variations and produce a phase-stabilized channel for spectroscopy. We have observed a number of protoplanetary disks using SPR and a grism providing a spectral dispersion of ~2000. In our data we spatially resolve emission from dust as well as gas. Hydrogen line emission is spectrally resolved, allowing differential phase measurements across the emission line that constrain the relative centroids of different velocity components at the 10 microarcsecond level. In the upcoming year, we will begin dual-field phase referencing (DFPR) measurements of the Galactic Center and a number of exoplanet systems. These observations will, in part, serve as precursors to astrometric monitoring of stellar orbits in the Galactic Center and stellar wobbles of exoplanet host stars. We describe the design of several scientific investigations capitalizing on the upcoming phase-referencing and astrometric capabilities of ASTRA.Comment: Published in the proceedings of the SPIE 2010 conference on "Optical and Infrared Interferometry II

Transcriptomic and ChIP-sequence interrogation of EGFR signaling in HER2+ breast cancer cells reveals a dynamic chromatin landscape and S100 genes as targets.

BACKGROUND:The Human Epidermal Growth Factor Receptor (EGFR/HER1) can be activated by several ligands including Transforming Growth Factor alpha (TGF-α) and Epidermal Growth Factor (EGF). Following ligand binding, EGFR heterodimerizes with other HER family members, such as HER2 (human epidermal growth factor receptor-2). Previously, we showed that the EGFR is upregulated in trastuzumab resistant HER2 positive (HER2+) breast cancer cells. This study is aimed to determine the downstream effects on transcription following EGFR upregulation in HER2+ breast cancer cells. METHODS:RNA-sequence and ChIP-sequence for H3K18ac and H3K27ac (Histone H3 lysine K18 and K27 acetylation) were conducted following an Epidermal Growth Factor (EGF) treatment time course in HER2+ breast cancer cells, SKBR3. The levels of several proteins of interest were confirmed by western blot analysis. The cellular localization of proteins of interest was examined using biochemically fractionated lysates followed by western blot analysis. RESULTS:Over the course of 24 h, EGFR stimulation resulted in the modulation of over 4000 transcripts. Moreover, our data demonstrates that EGFR/HER2 signaling regulates the epigenome, with global H3K18ac and H3K27ac oscillating as a function of time following EGF treatment. RNA-sequence data demonstrates the activation of immediate early genes (IEGs) and delayed early genes (DEGs) within 1 h of EGF treatment. More importantly, we have identified members of the S100 (S100 Calcium Binding Protein) gene family as likely direct targets of EGFR signaling as H3K18ac, H3K27ac and pol2 (RNA polymerase II) increase near the transcription start sites of some of these genes. CONCLUSIONS:Our data suggests that S100 proteins, which act as Ca2+ sensors, could play a role in EGF induced tumor cell growth and metastasis, contribute to trastuzumab resistance and cell migration and that they are likely drug targets in HER2+ breast cancer