Diffraction of complex molecules by structures made of light

We demonstrate that structures made of light can be used to coherently control the motion of complex molecules. In particular, we show diffraction of the fullerenes C60 and C70 at a thin grating based on a standing light wave. We prove experimentally that the principles of this effect, well known from atom optics, can be successfully extended to massive and large molecules which are internally in a thermodynamic mixed state and which do not exhibit narrow optical resonances. Our results will be important for the observation of quantum interference with even larger and more complex objects.Comment: 4 pages, 3 figure

Atom gratings produced by large angle atom beam splitters

An asymptotic theory of atom scattering by large amplitude periodic potentials is developed in the Raman-Nath approximation. The atom grating profile arising after scattering is evaluated in the Fresnel zone for triangular, sinusoidal, magneto-optical, and bichromatic field potentials. It is shown that, owing to the scattering in these potentials, two \QTR{em}{groups} of momentum states are produced rather than two distinct momentum components. The corresponding spatial density profile is calculated and found to differ significantly from a pure sinusoid.Comment: 16 pages, 7 figure

High resolution amplitude and phase gratings in atom optics

An atom-field geometry is chosen in which an atomic beam traverses a field interaction zone consisting of three fields, one having frequency $\Omega =c/\lambda$ propagating in the $\hat{z}$ direction and the other two having frequencies $\Omega +\delta_{1}$ and $\Omega +\delta_{2}$ propagating in the -$\hat{z}$ direction. For $n_{1}\delta_{1}+n_{2}\delta_{2}=0$ and $|\delta_{1}| T,|\delta_{2}| T\gg 1$, where $n_{1}$ and $n_{2}$ are positive integers and $T$ is the pulse duration in the atomic rest frame, the atom-field interaction results in the creation of atom amplitude and phase gratings having period $\lambda /[2(n_{1}+n_{2})]$. In this manner, one can use optical fields having wavelength $\lambda$ to produce atom gratings having periodicity much less than $\lambda$.Comment: 11 pages, 14 figure

Bloch-Like Quantum Multiple Reflections of Atoms

We show that under certain circumstances an atom can follow an oscillatory motion in a periodic laser profile with a Gaussian envelope. These oscillations can be well explained by using a model of energetically forbidden spatial regions. The similarities and differences with Bloch oscillations are discussed. We demonstrate that the effect exists not only for repulsive but also for attractive potentials, i.e. quantum multiple reflections are also possible.Comment: LaTeX, 7 pages, 7 figure

Talbot Oscillations and Periodic Focusing in a One-Dimensional Condensate

An exact theory for the density of a one-dimensional Bose-Einstein condensate with hard core particle interactions is developed in second quantization and applied to the scattering of the condensate by a spatially periodic impulse potential. The boson problem is mapped onto a system of free fermions obeying the Pauli exclusion principle to facilitate the calculation. The density exhibits a spatial focusing of the probability density as well as a periodic self-imaging in time, or Talbot effect. Furthermore, the transition from single particle to many body effects can be measured by observing the decay of the modulated condensate density pattern in time. The connection of these results to classical and atom optical phase gratings is made explicit

Bragg spectroscopy of a Bose-Einstein condensate

Properties of a Bose-Einstein condensate were studied by stimulated, two-photon Bragg scattering. The high momentum and energy resolution of this method allowed a spectroscopic measurement of the mean-field energy and of the intrinsic momentum uncertainty of the condensate. The coherence length of the condensate was shown to be equal to its size. Bragg spectroscopy can be used to determine the dynamic structure factor over a wide range of energy and momentum transfers.Comment: 4 pages, 3 figure

Planck's scale dissipative effects in atom interferometry

Atom interferometers can be used to study phenomena leading to irreversibility and dissipation, induced by the dynamics of fundamental objects (strings and branes) at a large mass scale. Using an effective, but physically consistent description in terms of a master equation of Lindblad form, the modifications of the interferometric pattern induced by the new phenomena are analyzed in detail. We find that present experimental devices can in principle provide stringent bounds on the new effects.Comment: 12 pages, plain-Te

Activity of Bdellovibrio Hit Locus Proteins, Bd0108 and Bd0109, Links Type IVa Pilus Extrusion/Retraction Status to Prey-Independent Growth Signalling

Bdellovibrio bacteriovorus are facultatively predatory bacteria that grow within gram-negative prey, using pili to invade their periplasmic niche. They also grow prey-independently on organic nutrients after undergoing a reversible switch. The nature of the growth switching mechanism has been elusive, but several independent reports suggested mutations in the hit (host-interaction) locus on the Bdellovibrio genome were associated with the transition to preyindependent growth. Pili are essential for prey entry by Bdellovibrio and sequence analysis of the hit locus predicted that it was part of a cluster of Type IVb pilus-associated genes, containing bd0108 and bd0109. In this study we have deleted the whole bd0108 gene, which is unique to Bdellovibrio, and compared its phenotype to strains containing spontaneous mutations in bd0108 and the common natural 42 bp deletion variant of bd0108. We find that deletion of the whole bd0108 gene greatly reduced the extrusion of pili, whereas the 42 bp deletion caused greater pilus extrusion than wild-type. The pili isolated from these strains were comprised of the Type IVa pilin protein; PilA. Attempts to similarly delete gene bd0109, which like bd0108 encodes a periplasmic/secreted protein, were not successful, suggesting that it is likely to be essential for Bdellovibrio viability in any growth mode. Bd0109 has a sugar binding YD- repeat motif and an N-terminus with a putative pilin-like fold and was found to interact directly with Bd0108. These results lead us to propose that the Bd0109/Bd0108 interaction regulates pilus production in Bdellovibrio (possibly by interaction with the pilus fibre at the cell wall), and that the presence (and possibly retraction state) of the pilus feeds back to alter the growth state of the Bdellovibrio cell. We further identify a novel small RNA encoded by the hit locus, the transcription of which is altered in different bd0108 mutation background

Effects of N-Glycosylation Site Removal in Archaellins on the Assembly and Function of Archaella in Methanococcus maripaludis

In Methanococcus maripaludis S2, the swimming organelle, the archaellum, is composed of three archaellins, FlaB1S2, FlaB2S2 and FlaB3S2. All three are modified with an N-linked tetrasaccharide at multiple sites. Disruption of the N-linked glycosylation pathway is known to cause defects in archaella assembly or function. Here, we explored the potential requirement of N-glycosylation of archaellins on archaellation by investigating the effects of eliminating the 4 N-glycosylation sites in the wildtype FlaB2S2 protein in all possible combinations either by Asn to Glu (N to Q) substitution or Asn to Asp (N to D) substitutions of the N-glycosylation sequon asparagine. The ability of these mutant derivatives to complement a non-archaellated ΔflaB2S2 strain was examined by electron microscopy (for archaella assembly) and swarm plates (for analysis of swimming). Western blot results showed that all mutated FlaB2S2 proteins were expressed and of smaller apparent molecular mass compared to wildtype FlaB2S2, consistent with the loss of glycosylation sites. In the 8 single-site mutant complements, archaella were observed on the surface of Q2, D2 and D4 (numbers after N or Q refer to the 1st to 4th glycosylation site). Of the 6 double-site mutation complementations all were archaellated except D1,3. Of the 4 triple-site mutation complements, only D2,3,4 was archaellated. Elimination of all 4 N-glycosylation sites resulted in non-archaellated cells, indicating some minimum amount of archaellin glycosylation was necessary for their incorporation into stable archaella. All complementations that led to a return of archaella also resulted in motile cells with the exception of the D4 version. In addition, a series of FlaB2S2 scanning deletions each missing 10 amino acids was also generated and tested for their ability to complement the ΔflaB2S2 strain. While most variants were expressed, none of them restored archaellation, although FlaB2S2 harbouring a smaller 3-amino acid deletion was able to partially restore archaellation