Reliability and validity of depth camera 3D scanning to determine thigh volume

Gross thigh volume is a key anthropometric variable to predict sport performance and health. Currently, it is either estimated by using the frustum method, which is prone to high inter-and intra-observer error, or using medical imaging, which is expensive and time consuming. Depth camera 3D-imaging systems offer a cheap alternative to measure thigh volume but no between-session reliability or comparison to medical imaging has been made. This experiment established between-session reliability and examined agreement with magnetic resonance imaging (MRI). Forty-eight male cyclists had their thigh volume measured by the depth camera system on two occasions to establish between-session reliability. A subset of 32 participants also had lower body MRIs, through which agreement between the depth camera system and MRI was established. The results showed low between-session variability (CV = 1.7%; Absolute Typical Error = 112 cm3) when measuring thigh volume using the depth camera system. The depth camera systematically measured gross thigh volume 32.6cm3 lower than MRI. These results suggest that depth camera 3D-imaging systems are reliable tools for measuring thigh volume and show good agreement with MRI scanners, providing a cheap and time-saving alternative to medical imaging analysis

Fabrication and optical characterization of pedestal micro-structures on DUV210 polymer: waveguides structures towards micro-resonators

International audienceThe paper presents the global design, fabrication and optical characterizations of pedestal waveguides and 2D microresonators made of DUV210 polymer. These particular geometries are achieved thanks to specific deep-UV lithography procedures allowing sub-lambda development coupled to chemical etching able to shape such pedestal configuration on various optical microstructures. Scanning electron microscopy images have confirmed their features. Moreover, such families of pedestal structures were characterized experimentally, including the optical losses measurements for the waveguides and the optical resonance responses for two kinds of microresonators. Optical studies of single mode propagation losses measurements have been performed by a cut back method leading to values close to 20 dB/cm at a 635 nm wavelength. Additionally, resonant spectral analysis has been performed into pedestal rings and racetracks microresonators with a broadband laser source centered at 795 nm, demonstrating the presence of expected theoretical resonances. Free spectral range values of 2.86 nm and 2.51 nm have been measured for these new designed pedestal resonators on DUV210 related to quality factor values superior to 520 and 610 respectively

Three years of harvest with the vector vortex coronagraph in the thermal infrared

For several years, we have been developing vortex phase masks based on sub-wavelength gratings, known as Annular Groove Phase Masks. Etched onto diamond substrates, these AGPMs are currently designed to be used in the thermal infrared (ranging from 3 to 13 {\mu}m). Our AGPMs were first installed on VLT/NACO and VLT/VISIR in 2012, followed by LBT/LMIRCam in 2013 and Keck/NIRC2 in 2015. In this paper, we review the development, commissioning, on-sky performance, and early scientific results of these new coronagraphic modes and report on the lessons learned. We conclude with perspectives for future developments and applications.Comment: To appear in SPIE proceedings vol. 990

High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein

Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)], a "transferrinfection" system for apo(a) was developed utilizing adenovirus receptor- and transferrin receptor-mediated DNA uptake into cells. Using this method, different apo(a) cDNA constructions of variable length, due to the presence of 3, 5, 7, 9, 15, or 18 internal kringle IV sequences, were expressed in cos-7 cells or CHO cells. All constructions contained kringle IV-36, which includes the only unpaired cysteine residue (Cys-4057) in apo(a). r-Apo(a) was synthesized as a precursor and secreted as mature apolipoprotein into the medium. When medium containing r-apo(a) with 9, 15, or 18 kringle IV repeats was mixed with normal human plasma LDL, stable complexes formed that had a bouyant density typical of Lp(a). Association was substantially decreased if Cys-4057 on r-apo(a) was replaced by Arg by site-directed mutagenesis or if Cys-4057 was chemically modified. Lack of association was also observed with r-apo(a) containing only 3, 5, or 7 kringle IV repeats without "unique kringle IV sequences", although Cys-4057 was present in all of these constructions. Synthesis and secretion of r-apo(a) was not dependent on its sialic acid content. r-Apo(a) was expressed even more efficiently in sialylation-defective CHO cells than in wild-type CHO cells. In transfected CHO cells defective in the addition of N-acetylglucosamine, apo(a) secretion was found to be decreased by 50%. Extracellular association with LDL was not affected by the carbohydrate moiety of r-apo(a), indicating a protein-protein interaction between r-apo(a) and apoB. These results show that, besides kringle IV-36, other kringle IV sequences are necessary for the extracellular association of r-apo(a) with LDL. Changes in the carbohydrate moiety of apo(a), however, do not affect complex formation

Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity

The role of ATP-binding Cassette G1 (ABCG1) transporter in human pathophysiology is still largely unknown. Indeed, beyond its role in mediating free cholesterol efflux to HDL, ABCG1 transporter equally promotes lipid accumulation in a triglyceride (TG)-rich environment through regulation of the bioavailability of Lipoprotein Lipase (LPL).As both ABCG1 and LPL are expressed in adipose tissue, we hypothesize that ABCG1 is implicated in adipocyte TG storage and could be then a major actor in adipose tissue fat accumulation.Silencing of Abcg1 expression by RNAi in 3T3-L1 preadipocytes compromised LPL-dependent TG accumulation during initial phase of differentiation. Generation of stable Abcg1 Knockdown 3T3-L1 adipocytes revealed that Abcg1 deficiency reduces TG storage and diminishes lipid droplet size through inhibition of Pparγ expression. Strikingly, local inhibition of adipocyte Abcg1 in adipose tissue from mice fed a high fat diet led to a rapid decrease of adiposity and weight gain. Analysis of two frequent ABCG1 SNPs (rs1893590 (A/C) and rs1378577 (T/G)) in morbidly obese individuals indicated that elevated ABCG1 expression in adipose tissue was associated with an increased PPARγ expression and adiposity concomitant to an increased fat mass and BMI (haplotype AT>GC). The critical role of ABCG1 regarding obesity was further confirmed in independent populations of severe obese and diabetic obese individuals.For the first time, this study identifies a major role of adipocyte ABCG1 in adiposity and fat mass growth and suggests that adipose ABCG1 might represent a potential therapeutic target in obesity

Exoplanet direct imaging in ground-based conditions on THD2 bench

The next generation of ground-based instruments aims to break through the knowledge we have on exoplanets by imaging circumstellar environments always closer to the stars. However, direct imaging requires an AO system and high-contrast techniques like a coronagraph to reject the diffracted light of an observed star and an additional wavefront sensor to control quasi-static aberrations, including the non common path aberrations. To observe faint objects, a focal plane wavefront sensor with a sub-nanometric wavefront control capability is required. In the past few years, we developed the THD2 bench which is a testbed for high-contrast imaging techniques, working in visible and near infrared wavelengths and currently reaching contrast levels lower than 1e-8 under space-like simulated conditions. We recently added a turbulence wheel on the optical path which simulates the residuals given by a typical extreme adaptive optics system and we tested several ways to remove quasi-statics speckles. One way to estimate the aberrations is a method called pair-wise probing where we record few images with known-shapes we apply on the adaptive optics deformable mirror. Once estimated, we seek to minimize the focal-plane electric field by an algorithm called Electric Field Conjugation. In this paper, we present the first results obtained on the THD2 bench using these two techniques together in turbulent conditions. We then compare the achieved performance with the one expected when all the quasi-static speckles are corrected.Comment: 9 pages, 3 figures, AO4ELT6 Qu\'ebec cit

Extremely Halophilic Archaeal Communities are Resilient to Short‐Term Entombment in Halite

Some haloarchaea avoid the harsh conditions present in evaporating brines by entombment in brine inclusions within forming halite crystals, where a subset of haloarchaea survives over geological time. However, shifts in the community structure of halite‐entombed archaeal communities remain poorly understood. Therefore, we analysed archaeal communities from in situ hypersaline brines collected from Trapani saltern (Sicily) and their successional changes in brines versus laboratory‐grown halite over 21 weeks, using high‐throughput sequencing. Haloarchaea were dominant, comprising >95% of the archaeal community. Unexpectedly, the OTU richness of the communities after 21 weeks was indistinguishable from the parent brine and overall archaeal abundance in halite showed no clear temporal trends. Furthermore, the duration of entombment was less important than the parent brine from which the halite derived in determining the community composition and relative abundances of most genera in halite‐entombed communities. These results show that halite‐entombed archaeal communities are resilient to entombment durations of up to 21 weeks, and that entombment in halite may be an effective survival strategy for near complete communities of haloarchaea. Additionally, the dominance of ‘halite specialists’ observed in ancient halite must occur over periods of years, rather than months, hinting at long‐term successional dynamics in this environment