Diagnóstico ecográfico en un caso clínico de quiste paraprostático canino

El artículo describe el diagnóstico ecográfico de un caso clínico de quiste paraprostático en un perro, discutiendo la importancia de la imagen en el diagnóstico de afecciones prostáticas y mostrando imágenes radiológicas y ecográficas.This paper describes the ultrasonographic diagnosis in a case of paraprostatic cyst in a dog. Importance of imaging diagnostic methods in prostatic diseases are discussed. Radiologic and ultrasound images are shown

Diagnóstico por imagen de un caso de invaginación intestinal

Aprovechando como base un caso clínico de intususcepción intestinal, se discuten las ventajas e inconvenientes de los dos métodos de diagnóstico por imagen más usuales en medicina veterinaria, como son la radiología y la ecografía.A clinical case of intestinal intussusception in a dog is described, emphatazing the imaging diagnosis and discussing about the advantages and disadvantages of the two most used methods, as radiology and ultrasonography

Marie Anne Paulze Lavoisier: unha descoñecida científica

[RESUMO] A contribución das mulleres á creación científica ao longo da historia da Humanidade non parece importante se consideramos o pouco que aparecen nos libros de texto; deste xeito, o alumnado non advertirá a existencia de mulleres que fixeron ou elaboraron leis ou teorías nas ciencias. Personaxes como: Hipatia de Alejandría, Hildegarda de Birgen, Caroline Herschel, María Cunitz, Marie Orr Evershed, Augusta Ada Byron, María Montagu, María Agnesi, Sophía Germain, Sonya Kovalevski, María Goeppter Mayer, Rosalin Franklin, Lise Meitner,..., son descoñecidas do gran público. Incluso aquelas que resultan ser coñecidas, como Irene ou Marie Curie, tampouco foron dabondo valoradas. Para recuperar estas mulleres científicas do silencio e do esquecemento e facelas visibles debemos sacar á luz as súas achegas. Este é o caso da protagonista da nosa comunicación. Marie-Anne Pierrette Paulze, que nos libros aparece como a esposa de Lavoisier, pero non como unha persoa con coñecementos científicos profundos como esperamos amosar. Presentamos as súas no mundo da química, que practicaba xunto ao seu home, e a todos cantos científicos formaban o chamado grupo do Arsenal

Clonal human fetal ventral mesencephalic dopaminergic neuron precursors for cell therapy research

A major challenge for further development of drug screening procedures, cell replacement therapies and developmental studies is the identification of expandable human stem cells able to generate the cell types needed. We have previously reported the generation of an immortalized polyclonal neural stem cell (NSC) line derived from the human fetal ventral mesencephalon (hVM1). This line has been biochemically, genetically, immunocytochemically and electrophysiologically characterized to document its usefulness as a model system for the generation of A9 dopaminergic neurons (DAn). Long-term in vivo transplantation studies in parkinsonian rats showed that the grafts do not mature evenly. We reasoned that diverse clones in the hVM1 line might have different abilities to differentiate. In the present study, we have analyzed 9 hVM1 clones selected on the basis of their TH generation potential and, based on the number of v-myc copies, v-myc down-regulation after in vitro differentiation, in vivo cell cycle exit, TH+ neuron generation and expression of a neuronal mature marker (hNSE), we selected two clones for further in vivo PD cell replacement studies. The conclusion is that homogeneity and clonality of characterized NSCs allow transplantation of cells with controlled properties, which should help in the design of long-term in vivo experimentsThis work was supported by grants from the Spanish Ministry of Economy and Competitiveness (formerly Science and Innovation; PLE2009-0101, SAF2010-17167), Comunidad Autónoma Madrid (S2011-BMD-2336), Instituto Salud Carlos III (RETICS TerCel, RD06/0010/0009) and European Union (Excell, NMP4-SL-2008-214706). This work was also supported by an institutional grant from Foundation Ramón Areces to the Center of Molecular Biology Severo Ocho

Human cortical organoids expose a differential function of GSK3 on cortical neurogenesis

The regulation of the proliferation and polarity of neural progenitors is crucial for the development of the brain cortex. Animal studies have implicated glycogen synthase kinase 3 (GSK3) as a pivotal regulator of both proliferation and polarity, yet the functional relevance of its signaling for the unique features of human corticogenesis remains to be elucidated. We harnessed human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Single-cell transcriptome profiling revealed a direct impact on early neurogenesis and uncovered a selective role of GSK3 in the regulation of glutamatergic lineages and outer radial glia output. Our dissection of the GSK3-dependent transcriptional network in human corticogenesis underscores the robustness of the programs determining neuronal identity independent of tissue architecture

WASP-14b: 7.3 <i>M</i>_J transiting planet in an eccentric orbit

We report the discovery of a 7.3 MJ exoplanet WASP-14b, one of the most massive transiting exoplanets observed to date. The planet orbits the 10th-magnitude F5V star USNO-B1?11118?0262485 with a period of 2.243?752 d and orbital eccentricity e= 0.09 . A simultaneous fit of the transit light curve and radial velocity measurements yields a planetary mass of 7.3 ± 0.5 MJ and a radius of 1.28 ± 0.08 RJ. This leads to a mean density of about 4.6 g?cm?3 making it the densest transiting exoplanets yet found at an orbital period less than 3 d. We estimate this system to be at a distance of 160 ± 20 pc. Spectral analysis of the host star reveals a temperature of 6475 ± 100 K, log?g= 4.07 cm?s?2 and v sin?i= 4.9 ± 1.0 km?s?1, and also a high lithium abundance, log?N(Li) = 2.84 ± 0.05 . The stellar density, effective temperature and rotation rate suggest an age for the system of about 0.5-1.0 Gyr

Juvenile obesity and its association with utilisation and costs of pharmaceuticals - results from the KiGGS study

<p>Abstract</p> <p>Background</p> <p>According to a national reference, 15% of German children and adolescents are overweight (including obese) and 6.3% are obese. An earlier study analysed the impact of childhood overweight and obesity on different components of direct medical costs (physician, hospital and therapists). To complement the existing literature for Germany, this study aims to explore the association of body mass index (BMI) with utilisation of pharmaceuticals and related costs in German children and adolescents.</p> <p>Methods</p> <p>Based on data from 14, 836 respondents aged 3-17 years in the German Interview and Examination Survey for Children and Adolescents (KiGGS), drug intake and associated costs were estimated using a bottom-up approach. To investigate the association of BMI with utilisation and costs, univariate analyses and multivariate generalised mixed models were conducted.</p> <p>Results</p> <p>There was no significant difference between BMI groups regarding the probability of drug utilisation. However, the number of pharmaceuticals used was significantly higher (14%) for obese children than for normal weight children. Furthermore, there was a trend for more physician-prescribed medication in obese children and adolescents. Among children with pharmaceutical intake, estimated costs were 24% higher for obese children compared with the normal weight group.</p> <p>Conclusions</p> <p>This is the first study to estimate excess drug costs for obesity based on a representative cross-sectional sample of the child and adolescent population in Germany. The results suggest that obese children should be classified as a priority group for prevention. This study complements the existing literature and provides important information concerning the relevance of childhood obesity as a health problem.</p

Serological Studies Confirm the Novel Astrovirus HMOAstV-C as a Highly Prevalent Human Infectious Agent

Molecular identification of a microbe is the first step in determining its prevalence of infection and pathogenic potential. Detection of specific adaptive immune responses can provide insights into whether a microbe is a human infectious agent and its epidemiology. Here we characterized human anti-IgG antibody responses by luciferase immunoprecipitation systems (LIPS) against two protein fragments derived from the capsid protein of the novel HMOAstV-C astrovirus. While antibodies to the N-terminal fragment were not informative, the C-terminal capsid fragment of HMOAstV-C showed a high frequency of immunoreactivity with serum from healthy blood donors. In contrast, a similar C-terminal capsid fragment from the related HMOAstV-A astrovirus failed to show immunoreactivity. Detailed analysis of adult serum from the United Sates using a standardized threshold demonstrated HMOAstV-C seropositivity in approximately 65% of the samples. Evaluation of serum samples from different pediatric age groups revealed that the prevalence of antibodies in 6–12 month, 1–2 year, 2–5 year and 5–10 year olds was 20%, 23%, 32% and 36%, respectively, indicating rising seroprevalence with age. Additionally, 50% (11/22) of the 0–6 month old children showed anti-HMOAstV-C antibody responses, likely reflecting maternal antibodies. Together these results document human humoral responses to HMOAstV-C and validate LIPS as a facile and effective approach for identifying humoral responses to novel infectious agents