Machine learning for automatic prediction of the quality of electrophysiological recordings

The quality of electrophysiological recordings varies a lot due to technical and biological variability and neuroscientists inevitably have to select “good” recordings for further analyses. This procedure is time-consuming and prone to selection biases. Here, we investigate replacing human decisions by a machine learning approach. We define 16 features, such as spike height and width, select the most informative ones using a wrapper method and train a classifier to reproduce the judgement of one of our expert electrophysiologists. Generalisation performance is then assessed on unseen data, classified by the same or by another expert. We observe that the learning machine can be equally, if not more, consistent in its judgements as individual experts amongst each other. Best performance is achieved for a limited number of informative features; the optimal feature set being different from one data set to another. With 80–90% of correct judgements, the performance of the system is very promising within the data sets of each expert but judgments are less reliable when it is used across sets of recordings from different experts. We conclude that the proposed approach is relevant to the selection of electrophysiological recordings, provided parameters are adjusted to different types of experiments and to individual experimenters

BLUF Domain Function Does Not Require a Metastable Radical Intermediate State

BLUF (blue light using flavin) domain proteins are an important family of blue light-sensing proteins which control a wide variety of functions in cells. The primary light-activated step in the BLUF domain is not yet established. A number of experimental and theoretical studies points to a role for photoinduced electron transfer (PET) between a highly conserved tyrosine and the flavin chromophore to form a radical intermediate state. Here we investigate the role of PET in three different BLUF proteins, using ultrafast broadband transient infrared spectroscopy. We characterize and identify infrared active marker modes for excited and ground state species and use them to record photochemical dynamics in the proteins. We also generate mutants which unambiguously show PET and, through isotope labeling of the protein and the chromophore, are able to assign modes characteristic of both flavin and protein radical states. We find that these radical intermediates are not observed in two of the three BLUF domains studied, casting doubt on the importance of the formation of a population of radical intermediates in the BLUF photocycle. Further, unnatural amino acid mutagenesis is used to replace the conserved tyrosine with fluorotyrosines, thus modifying the driving force for the proposed electron transfer reaction; the rate changes observed are also not consistent with a PET mechanism. Thus, while intermediates of PET reactions can be observed in BLUF proteins they are not correlated with photoactivity, suggesting that radical intermediates are not central to their operation. Alternative nonradical pathways including a keto–enol tautomerization induced by electronic excitation of the flavin ring are considered

Search for hidden-photon dark matter with the FUNK experiment

Many extensions of the Standard Model of particle physics predict a parallel sector of a new U(1) symmetry, giving rise to hidden photons. These hidden photons are candidate particles for cold dark matter. They are expected to kinetically mix with regular photons, which leads to a tiny oscillating electric-field component accompanying dark matter particles. A conducting surface can convert such dark matter particles into photons which are emitted almost perpendicularly to the surface. The corresponding photon frequency follows from the mass of the hidden photons. In this contribution we present a preliminary result on a hidden photon search in the visible and near-UV wavelength range that was done with a large, 14 m2 spherical metallic mirror and discuss future dark matter searches in the eV and sub-eV range by application of different detectors for electromagnetic radiation.Comment: Contribution to the 35th International Cosmic Ray Conference ICRC2017, 10 to 20 July, 2017, Bexco, Busan, Korea. arXiv admin note: text overlap with arXiv:1711.0296

Primary Proton Spectrum of Cosmic Rays measured with Single Hadrons

The flux of cosmic-ray induced single hadrons near sea level has been measured with the large hadron calorimeter of the KASCADE experiment. The measurement corroborates former results obtained with detectors of smaller size if the enlarged veto of the 304 m^2 calorimeter surface is encounted for. The program CORSIKA/QGSJET is used to compute the cosmic-ray flux above the atmosphere. Between E_0=300 GeV and 1 PeV the primary proton spectrum can be described with a power law parametrized as dJ/dE_0=(0.15+-0.03)*E_0^{-2.78+-0.03} m^-2 s^-1 sr^-1 TeV^-1. In the TeV region the proton flux compares well with the results from recent measurements of direct experiments.Comment: 13 pages, accepted by Astrophysical Journa

Large scale cosmic-ray anisotropy with KASCADE

The results of an analysis of the large scale anisotropy of cosmic rays in the PeV range are presented. The Rayleigh formalism is applied to the right ascension distribution of extensive air showers measured by the KASCADE experiment.The data set contains about 10^8 extensive air showers in the energy range from 0.7 to 6 PeV. No hints for anisotropy are visible in the right ascension distributions in this energy range. This accounts for all showers as well as for subsets containing showers induced by predominantly light respectively heavy primary particles. Upper flux limits for Rayleigh amplitudes are determined to be between 10^-3 at 0.7 PeV and 10^-2 at 6 PeV primary energy.Comment: accepted by The Astrophysical Journa

KASCADE: Astrophysical results and tests of hadronic interaction models

KASCADE is a multi-detector setup to get redundant information on single air shower basis. The information is used to perform multiparameter analyses to solve the threefold problem of the reconstruction of (i)the unknown primary energy, (ii) the primary mass, and (iii) to quantify the characteristics of the hadronic interactions in the air-shower development. In this talk recent results of the KASCADE data analyses are summarized concerning cosmic ray anisotropy studies, determination of flux spectra for different primary mass groups, and approaches to test hadronic interaction models. Neither large scale anisotropies nor point sources were found in the KASCADE data set. The energy spectra of the light element groups result in a knee-like bending and a steepening above the knee. The topology of the individual knee positions shows a dependency on the primary particle. Though no hadronic interaction model is fully able to describe the multi-parameter data of KASCADE consistently, the more recent models or improved versions of older models reproduce the data better than few years ago.Comment: to appear in Nucl. Phys. B (Proc. Suppl.), Proc. of the XIII ISVHECRI, Pylos 2004 - with a better quality of the figure

Dissecting the knee - Air shower measurements with KASCADE

Recent results of the KASCADE air shower experiment are presented in order to shed some light on the astrophysics of cosmic rays in the region of the knee in the energy spectrum. The results include investigations of high-energy interactions in the atmosphere, the analysis of the arrival directions of cosmic rays, the determination of the mean logarithmic mass, and the unfolding of energy spectra for elemental groups

First results of the air shower experiment KASCADE

The main goals of the KASCADE (KArlsruhe Shower Core and Array DEtector) experiment are the determination of the energy spectrum and elemental composition of the charged cosmic rays in the energy range around the knee at ca. 5 PeV. Due to the large number of measured observables per single shower a variety of different approaches are applied to the data, preferably on an event-by-event basis. First results are presented and the influence of the high-energy interaction models underlying the analyses is discussed.Comment: 3 pages, 3 figures included, to appear in the TAUP 99 Proceedings, Nucl. Phys. B (Proc. Suppl.), ed. by M. Froissart, J. Dumarchez and D. Vignau