H2S paper strip method - A bacteriological test for faecal coliforms in drinking water at various temperatures

Epidemics arising from waterborne diseases are a global health problem. Faecal contamination of drinking water is the main cause of these outbreaks. According to WHO (1996) for drinking water to be safe, a 100 ml sample should not contain any coliform bacteria. The standard methods currently used for routine testing have many limitations especially when applied in remote areas. The H2S method has been developed as an on-site, inexpensive and easy to use method to test drinking water for remote and rural areas. The present work analyses the reliability of the H2S method for detecting faecal contamination in drinking water. The minimum level of faecal coliforms that could be detected and the incubation period required at various levels of contamination were studied. The range of temperatures at which the method was effective and the incubation period required at various temperatures were also determined. The H2S method was found to be able to detect contamination down to a level of 1 CFU/100 ml of coliform bacteria. Although the H2S method could be used at a temperature range of 20 to 44oC, temperatures between 28 to 37oC gave faster results. An incubation period of only 24 hours was required at 37oC, which was found to be the most suitable incubation temperature. The incubation period increased with a decrease or increase in temperature

Cosmological horizon entropy and generalised second law for flat Friedmann Universe

We discuss the generalized second law (GSL) and the constraints imposed by it for two types of Friedmann universes. The first one is the Friedmann universe with radiation and a positive cosmological constant, and the second one consists of non-relativistic matter and a positive cosmological constant. The time evolution of the event horizon entropy and the entropy of the contents within the horizon are analyses in an analytical way by obtaining the Hubble parameter. It is shown that the GSL constraint the temperature of both the radiation and matter of the Friedmann universe. It is also shown that, even though the net entropy of the radiation (or matter) is decreasing at sufficiently large times as the universe expand, it exhibit an increase during the early times when universe is decelerating. That is the entropy of the radiation within the comoving volume is decreasing only when the universe has got an event horizon.Comment: 15 pages, 9 figure