Evidence for dynamic and multiple roles for huntingtin in Ciona intestinalis

Although mutations in the huntingtin gene (HTT) due to poly-Q expansion cause neuropathology in humans (Huntington's disease; HD), the normal function(s) of the gene and its protein (HTT) remain obscure. With new information from recently sequenced invertebrate genomes, the study of new animal models opens the possibility of a better understanding of HTT function and its evolution. To these ends, we studied huntingtin expression pattern and dynamics in the invertebrate chordate Ciona intestinalis. Ciona huntingtin (Ci-HTT) shows a biphasic expression pattern during larval development and prior to metamorphosis. A single form of huntingtin protein is present until the early larval stages, at which time two different mass proteins become evident in the metamorphically competent larva. An antibody against Ci-HTT labeled 50 cells in the trunk mesenchyme regions in pre-hatching and hatched larvae and probably represents the distribution of the light form of the protein. Dual labeling with anti-Ci-HTT and anti-aldoketoreductase confirmed the presence of Ci-HTT in mesenchyme cells. Suppression of Ci-HTT RNA by a morpholino oligonucleotide reduced the number and apparent mobility of Ci-HTT positive cells. In Ciona, HTT expression has a dynamic temporal and spatial expression pattern that in ontogeny precedes metamorphosis. Although our results may reflect a derived function for the protein in pre- and post-metamorphic events in Ciona, we also note that as in vertebrates, there is evidence for multiple differential temporal expression, indicating that this protein probably has multiple roles in ontogeny and cell migration. © 2013 Springer-Verlag Berlin Heidelberg

Mass-Galaxy offsets in Abell 3827, 2218 and 1689: intrinsic properties or line-of-sight substructures?

We have made mass maps of three strong-lensing clusters, Abell 3827, Abell 2218 and Abell 1689, in order to test for mass-light offsets. The technique used is GRALE, which enables lens reconstruction with minimal assumptions, and specifically with no information about the cluster light being given. In the first two of these clusters, we find local mass peaks in the central regions that are displaced from the nearby galaxies by a few to several kpc. These offsets {\em could\/} be due to line of sight structure unrelated to the clusters, but that is very unlikely, given the typical levels of chance line-of-sight coincidences in $\Lambda CDM$ simulations --- for Abell 3827 and Abell 2218 the offsets appear to be intrinsic. In the case of Abell 1689, we see no significant offsets in the central region, but we do detect a possible line of sight structure: it appears only when sources at z\ga 3 are used for reconstructing the mass. We discuss possible origins of the mass-galaxy offsets in Abell 3827 and Abell 2218: these include pure gravitational effects like dynamical friction, but also non-standard mechanisms like self-interacting dark-matter.Comment: 14 pages, 9 figures; Accepted for publication in MNRA

Highly efficient spin-orbit torque and switching of layered ferromagnet Fe3GeTe2

Among van der Waals (vdW) layered ferromagnets, Fe3GeTe2 (FGT) is an excellent candidate material to form FGT/heavy metal heterostructures for studying the effect of spin-orbit torques (SOT). Its metallicity, strong perpendicular magnetic anisotropy built in the single atomic layers, relatively high Curie temperature (Tc about 225 K) and electrostatic gate tunability offer a tantalizing possibility of achieving the ultimate high SOT limit in monolayer all-vdW nanodevices. The spin current generated in Pt exerts a damping-like SOT on FGT magnetization. At about 2.5x1011 A/m2 current density,SOT causes the FGT magnetization to switch, which is detected by the anomalous Hall effect of FGT. To quantify the SOT effect, we measure the second harmonic Hall responses as the applied magnetic field rotates the FGT magnetization in the plane. Our analysis shows that the SOT efficiency is comparable with that of the best heterostructures containing three-dimensional (3D) ferromagnetic metals and much larger than that of heterostructures containing 3D ferrimagnetic insulators. Such large efficiency is attributed to the atomically flat FGT/Pt interface, which demonstrates the great potential of exploiting vdW heterostructures for highly efficient spintronic nanodevices

New connection formulae for the q-orthogonal polynomials via a series expansion of the q-exponential

Using a realization of the q-exponential function as an infinite multiplicative sereis of the ordinary exponential functions we obtain new nonlinear connection formulae of the q-orthogonal polynomials such as q-Hermite, q-Laguerre and q-Gegenbauer polynomials in terms of their respective classical analogs.Comment: 14 page

Matrix Metalloproteinases and Glaucoma Treatment.

Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that degrade extracellular matrix (ECM) components such as collagen and have important roles in multiple biological processes, including development and tissue remodeling, both in health and disease. The activity of MMPs is influenced by the expression of MMPs and tissue inhibitors of metalloproteinase (TIMPs). In the eye, MMP-mediated ECM turnover in the juxtacanalicular region of the trabecular meshwork (TM) reduces outflow resistance in the conventional outflow pathway and helps maintain intraocular pressure (IOP) homeostasis. An imbalance in the MMP/TIMP ratio may be involved in the elevated IOP often associated with glaucoma. The prostaglandin analog/prostamide (PGA) class of topical ocular hypotensive medications used in glaucoma treatment reduces IOP by increasing outflow through both conventional and unconventional (uveoscleral) outflow pathways. Evidence from in vivo and in vitro studies using animal models and anterior segment explant and cell cultures indicates that the mechanism of IOP lowering by PGAs involves increased MMP expression in the TM and ciliary body, leading to tissue remodeling that enhances conventional and unconventional outflow. PGA effects on MMP expression are dependent on the identity and concentration of the PGA. An intracameral sustained-release PGA implant (Bimatoprost SR) in development for glaucoma treatment can reduce IOP for many months after expected intraocular drug bioavailability. We hypothesize that the higher concentrations of bimatoprost achieved in ocular outflow tissues with the implant produce greater MMP upregulation and more extensive, sustained MMP-mediated target tissue remodeling, providing an extended duration of effect

Performance Analysis of Best Relaying Protocol Selection with Interferences at Relays

In this paper, we investigate the performance of selecting the best protocol between amplify and forward (AF) and decode and forward (DF) in multiple relay networks with multiple interferences at relays. In the selection scheme, the best protocol between AF and DF is selected depending on the comparisons of signal-to-interference and noise ratio (SINR) for all source-relay links. All relays measure the received SINR to decide forwarding signal or not. When SINR is above a certain threshold then DF is used otherwise AF is used. Particularly, we develop an accurate mathematical model for best relaying protocol by considering the effect of interferences to our scheme. Firstly, we derive the asymptotic closed form expression for the symbol error rate (SER) for the system under study. Also we derive an upper and lower bound of symbol error rate and show how they were tight with exact SER. Furthermore an approximate expression for the outage probability is derived. Numerical results are finally presented to validate the theoretical analysis with a different number of relays