22 research outputs found

    Letʼs Play Ball

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    Response of a String to an Accelerating Mass

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    The response of a string to a mass particle undergoing a constant horizontal acceleration from rest has been calculated. The string deflection is expressed in terms of the transverse mass motion. A delay-differential equation is solved both numerically and asymptotically for the mass velocity. String profiles are presented at subsonic and supersonic speeds. Two oppositely traveling jumps in string displacement are found to appear as the mass is accelerated through the wave speed of the string.</jats:p

    Use of artificial neural networks for engineering analysis of complex physical systems

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    Co-Culture of Human Keratinocytes on Post-Mitotic Human Dermal Fibroblast Feeder Cells: Production of Large Amounts of Interleukin 6

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    A large synthesis of human IL-6 was demonstrated in co-cultures of human keratinocytes on post-mitotic human dermal fibroblast (HDF) feeder layers. Immunoreactive IL-1β could be detected in the co-cultures and the addition of rabbit anti IL-1β antibodies to the co-cultures considerably reduced the IL-6 synthesis, suggesting that it was induced by endogenous IL-1β. Addition of saturating concentrations of IL-1β to HDF feeder layers as well as to subcultures of keratinocytes induced in both similar but moderate IL-6 production. Conditioned medium from keratinocyte cultures induced IL-6 secretion in HDF feeder cells, whereas the conditioned medium from HDF feeder layers led to only minimal increase of keratinocyte IL-6 production. The co-cultures of keratinocytes on HDF feeder layers produced much larger amounts of IL-6 than the sum of the IL-6 produced by the feeder cell and keratinocyte cultures after the addition of IL-1β. The co-cultures of keratinocytes with HDF feeder layers separated by a permeable membrane in a two-chamber system produced significantly lower amounts of IL-6 than the unseparated co-cultures. These findings indicate that a direct cell contact between keratinocytes and feeder cells is involved in the overproportioned increase of IL-6 production and secretion into the medium
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