Molecular Docking of Compounds From Chaetomium SP. Against Human Estrogen Receptor Alpha in Searching Anti Breast Cancer

A study on molecular docking-based virtual screening has been conducted to select virtual hit of compounds, reported its existence in fungal endophytes of Chaetomium sp. as cytotoxic agent of breast cancer. The ligands were docked into Human Estrogen Receptor alpha (HERa) as the protein which regulates the breast cancer growth via estradiol-estrogen receptor binding intervention. The results showed that two compounds bearing xanthone and two compounds bearing benzonaphtyridinedione scaffolds were selected as virtual hit ligands for HERa leading to the conclusion that these compounds were good to be developed as anti breast cancer

Penelusuran Potensi Aktivitas Sitotoksik Fraksi Kulit Batang Tumbuhan Faloak (Sterculia Quadrifida R.br)

People on Timor island use Faloak (Sterculia quadrifida R.Br.) bark as herbal remedy to cure diseases such as liver diseases, gastroenteritis and as stamina booster. This study aims to analyze the ability of anticancer fraction of faloak bark ethanol extract. The mashed bark of faloak plants extracted by maceration method using ethanol. The ethanol extract fractionated using preparative thin layer chromatography with mixture mobile phase which, chloroform, n-butanol, ethyl acetate with ratio (3: 4: 1,5). Chromatogram detected with visible light, UV254, UV366, and cerium sulfate reagent, then marked. Fraction obtained five fractions. Cytotoxic test against T47D cell and Vero cell conducted using MTT, IC50 and selectivity sndex (SI) used as an anticancer efficacy parameter. Fraction 4 showed moderate anticancer activity with IC50 of 21.89 μg/mL in T47D cell line

Fitoterapi dalam Sistem Kesehatan

Buku ajar berjudul Fitoterapi dalam Sistem Kesehatan merupakan buku yang membahas secara mendalam peran terapi berbasis tumbuhan dalam mendukung sistem pelayanan kesehatan modern. Buku ini menguraikan prinsip-prinsip ilmiah di balik efektivitas tanaman obat, mulai dari kandungan fitokimia aktif, mekanisme kerja biologis, hingga potensi penggunaannya dalam pencegahan dan pengobatan berbagai penyakit. Selain meninjau aspek farmakologis dan fisiologis, buku ini juga mengulas bagaimana fitoterapi dapat diintegrasikan dengan pelayanan medis konvensional melalui regulasi, pengawasan mutu, serta edukasi masyarakat. Pembaca juga akan diajak memahami pentingnya penelitian berkelanjutan, keamanan penggunaan bahan alam, dan peran tenaga kesehatan dalam memberikan edukasi terkait terapi herbal yang bertanggung jawab. Disajikan dengan pendekatan ilmiah namun mudah dipahami, buku ini menjadi bacaan penting bagi siapa pun yang ingin mengenal peran fitoterapi dalam meningkatkan kualitas hidup. Buku ini menegaskan bahwa keseimbangan antara pengetahuan tradisional dan sains modern merupakan kunci dalam membangun sistem kesehatan yang holistik, berkelanjutan, dan berpihak pada kesejahteraan manusia

Penelusuran Potensi Aktifitas Antioksidan Jantung Pisang Kepok (Musa Paradisiaca L.)

INTISARI Antioksidan merupakan zat yang mampu memperlambat atau mencegah proses oksidasi. Zat ini secara nyata mampu memperlambat atau menghambat oksidasi zat yang mudah teroksidasi meskipun dalam konsentrasi rendah. Antioksidan juga sesuai didefinisikan sebagai senyawa-senyawa yang melindungi sel dari efek berbahaya radikal bebas oksigen reaktif jika berkaitan dengan penyakit, radikal bebas ini dapat berasal dari metabolisme tubuh maupun faktor eksternal lainnya Radikal bebas adalah spesies yang tidak stabil karena memiliki elektron yang tidak berpasangan dan mencari pasangan elektron dalam makromolekul biologi. Protein lipida dan DNA dari sel manusia yang sehat merupakan sumber pasangan elektron yang baik.Kondisi oksidasi dapat menyebabkan kerusakan protein dan DNA, kanker, penuaan, dan penyakit lainnya.Komponen kimia yang berperan sebagai antioksidan adalah senyawa golongan fenolik dan polifenolik.Senyawa-senyawa golongan tersebut banyak terdapat dialam, terutama pada tumbuh-tumbuhan, dan memiliki kemampuan untuk menangkap radikal bebas.Antioksidan yang banyak ditemukan pada bahan pangan, antara lain vitamin E, vitamin C, dan karotenoid

Sterculia foetida leaf fraction against matrix metalloproteinase-9 protein and 4T1 breast cancer cells: In-vitro and in-silico studies

Sterculia foetida leaf extract has been shown to have cytotoxic activity. Matrix metalloproteinase-9 (MMP-9) has an important role in pathophysiological functions. Inhibition of MMP9 is an important therapeutic approach for combating cancer. This study was conducted to determine the most active fraction of S. foetida as anti-breast cancer agent with hemopexin-like domain of MMP-9 (PEX9) as the selective protein target and 4T1 cells line as metastatic breast cancer cell. The leaves S. foetida was extracted using 80% methanol and was fractionated into fractions of n-hexane, chloroform, ethyl acetate, n-butanol, and insoluble n-butanol with liquid-liquid partition. In vitro screening against MMP-9 was performed using FRET-based assay and cytotoxic tests were performed using the MTT assay. Identification of compounds in the most active fraction using GC-MS. The docking to PEX9 was run using AutoDock Vina embedded in PyRx program. The n-hexane fraction was the most active fraction to inhibit MMP-9 with an IC50 of 19.67 µg/mL and inhibit the growth of 4T1 cells with an IC50 of 34.65 µg/mL. NNGH was used as positive control for the in-vitro and in-silico studies. The GC-MS results of the n-hexane fraction showed that there were 23 compounds, and they had binding affinity score of -8.9 to -4.9 kcal/mol towards PEX9. It can be concluded that S. foetida leaf has the potential to be developed for therapeutic use, especially for breast cancer therapy

Pterygota alata (Roxb.) R.Br. bark fraction induced intrinsic apoptotic pathway in 4T1 cells by decreasing Bcl-2 and inducing bax expression

Background and Objective: Pterygota alata (Roxb.) R.Br. bark extract has been studied to have cytotoxic activity on 4T1 cells. This study was conducted to determine the cytotoxic activity of several fractions of Pterygota alata (Roxb.) R.Br. bark against 4T1 breast cancer cells and to investigate the most active fractions on Bcl-2 and Bax expressions. Materials and Methods: The bark of Pterygota alata (Roxb.) R.Br. was extracted using 80% methanol and was fractionated into fractions of n-hexane, chloroform, ethyl acetate, n-butanol and insoluble n-butanol with liquid-liquid partition. Cytotoxic tests were performed using the MTT method and expressions of Bax and Bcl-2 on 4T1 breast cancer cells were detected with immunocytochemical staining. Identification of compounds in the most active fraction using GC-MS. Results: The results showed that the most active fraction was the insoluble fraction of n-butanol (IFB) with an IC50 of 15.14 μg mL–1. IFB also decreases the expression of Bcl-2 and increases the expression of Bax. Conclusion: It can be concluded that Pterygota alata (Roxb.) R.Br. bark has the potential to be developed for medical use, especially for breast cancer therapy