Relationship of lipoprotein(a) to variables of coagulation and fibrinolysis in a healthy population

Abstract In the Prospective Cardiovascular Münster (PROCAM) study, serum lipoprotein(a) [Lp(a)] and its relationship to pro- and anticoagulatory as well as fibrinolytic indices were determined in a large group of employees: 864 men (m) and 373 women (f), ages 16-65 years. Univariate statistical analysis showed Lp(a) concentration to be associated with fibrinogen concentrations in both sexes (m: r = 0.08, P less than 0.05; f: r = 0.20, P less than 0.001), but not with euglobulin fibrinolysis activity, tissue-type plasminogen activator, plasminogen activator inhibitor type 1 (PAI-1), or the split products of cross-linked fibrin (d-dimer). In women only, Lp(a) was significantly correlated with antithrombin III (r = 0.15, P less than 0.01) and Protein C (r = 0.17, P less than 0.01). Further sex-related differences were seen in the relationship between Lp(a) and age (m: r = 0.05; f: r = 0.23, P less than 0.001) and body mass index (m: r = 0.01; f: r = 0.19, P less than 0.001), primarily as a consequence of remarkable differences of Lp(a) concentrations between postmenopausal (mean = 79.4 mg/L) and premenopausal women (mean = 51.5 mg/L, P = 0.001). Multiple-regression analysis demonstrated a significant negative correlation of Lp(a) to PAI-1 (m: beta = -0.12, P less than 0.01; f: beta = -0.14, P less than 0.05) and a positive correlation to cholesterol (m: beta = 0.18, P less than 0.001; f: beta = 0.17, P less than 0.01) and systolic blood pressure (m: beta = 0.08, P less than 0.05; f: beta = 0.11, P less than 0.05).</jats:p

A new high resolution radioimager for the quantitative analysis of radiolabelled molecules in tissue section

We present a high-speed, high-resolution imager of beta particles. It is devoted to be used in autoradiography experiments such as receptor binding or in situ hybridization experiments, either instead of, or in complement with autoradiographic film and emulsions. It allows the user to locate and perform quantitative analyses of (H-3, C-14, S-35, P-33, P-32, I-125) labelled molecules with a 15 mu m spatial resolution on a 0.9 x 1.3 cm(2) sensitive area. Combining recent techniques (specific scintillator thin sheets and intensified charge-coupled device (CCD)) this imager offers a wide dynamic range and real-time acquisitio

The role of affective control, strategy repertoire and subjective emotion regulation success in developmental internalising psychopathology

Abstract Adolescence poses significant challenges for emotion regulation (ER) and is thus a critical phase in the emergence of various mental disorders, specifically internalising disorders such as anxiety and depression. Affective control, defined as the application of cognitive control in affective contexts, is crucial for effective ER. However, the relationship between ER and affective control is unclear. This study examined the predictive role of ER strategies and difficulties in affective control, measured as the congruency effect and error rate on an Emotional Stroop task (EST), in a sample of adolescents and young adults (aged 14–21, M = 17.28, 22% male). It was hypothesised that participants with internalising disorders would show higher congruency effects and error rates on the EST than healthy controls after a psychosocial stress induction, indicating lower affective control. Surprisingly, our findings revealed no significant differences in these measures between the groups. However, higher depression scores were associated with increased EST errors. While ER strategies and difficulties did not predict affective control, exploratory analyses unveiled associations between depression scores and ER strategy repertoire, perceived ER success and the ER strategy Acceptance. These findings underscore the importance of implicit ER facets, particularly perceived ER success and flexibility to change between applied strategies for adolescents and young adults with elevated depressive symptoms

A new high resolution radioimager for the quantitative analysis of radiolabelled molecules in tissue section

We present a high-speed, high-resolution imager of beta particles. It is devoted to be used in autoradiography experiments such as receptor binding or in situ hybridization experiments, either instead of, or in complement with autoradiographic film and emulsions. It allows the user to locate and perform quantitative analyses of (H-3, C-14, S-35, P-33, P-32, I-125) labelled molecules with a 15 mu m spatial resolution on a 0.9 x 1.3 cm(2) sensitive area. Combining recent techniques (specific scintillator thin sheets and intensified charge-coupled device (CCD)) this imager offers a wide dynamic range and real-time acquisitio

Defined monoclonal antibodies to Escherichia coli β-galactosidase as a tool for characterisation of recombinant expression products

AbstractMouse monoclonal antibodies were prepared against β-galactosidase (EC 3.2.1.23) of Escherichia coli. The binding sites of these monoclonal antibodies within the β-galactosidase molecule were estimated by immunoblot analyses to various defined peptide regions of β-galactosidase, encoded by expression plasmids. Monoclonal antibodies, were characterised, which either bind to the amino-terminal or to the carboxy-terminal region or to an internal section of β-galactosidase. These defined monoclonal antibodies were shown to be a useful tool for characterisation of β-galactosidase fusion proteins expressed in Escherichia coli.Galactosidase, β-; Monoclonal antibody; Recombinant expression product; Epitope mapping; (E. coli