भारत में शूलचर्मी पालन में संभावनाएं

Prospects of Echiniculture in Indi

Cortical AAV-CNTF gene therapy combined with intraspinal mesenchymal precursor cell transplantation promotes functional and morphological outcomes after spinal cord injury in adult rats

Ciliary neurotrophic factor (CNTF) promotes survival and enhances long-distance regeneration of injured axons in parts of the adult CNS. Here we tested whether CNTF gene therapy targeting corticospinal neurons (CSN) in motor-related regions of the cerebral cortex promotes plasticity and regrowth of axons projecting into the female adult F344 rat spinal cord after moderate thoracic (T10) contusion injury (SCI). Cortical neurons were transduced with a bicistronic adeno-associated viral vector (AAV1) expressing a secretory form of CNTF coupled to mCHERRY (AAV-CNTFmCherry) or with control AAV only (AAV-GFP) two weeks prior to SCI. In some animals, viable or nonviable F344 rat mesenchymal precursor cells (rMPCs) were injected into the lesion site two weeks after SCI to modulate the inhibitory environment. Treatment with AAV-CNTFmCherry, as well as with AAV-CNTFmCherry combined with rMPCs, yielded functional improvements over AAV-GFP alone, as assessed by open-field and Ladderwalk analyses. Cyst size was significantly reduced in the AAV-CNTFmCherry plus viable rMPC treatment group. Cortical injections of biotinylated dextran amine (BDA) revealed more BDA-stained axons rostral and alongside cysts in the AAV-CNTFmCherry versus AAV-GFP groups. After AAV-CNTFmCherry treatments, many sprouting mCherry-immunopositive axons were seen rostral to the SCI, and axons were also occasionally found caudal to the injury site. These data suggest that CNTF has the potential to enhance corticospinal repair by transducing parent CNS populations

Carrier shifting algorithms for the mitigation of circulating current in diode clamped MLI fed induction motor drive

Abstract: Reduction of circulating current is one of the major considerations in inverter fed electrical drives. Diode clamped MLI enables higher output current per phase, thereby rating of the drive gets increased effectively. Various methods of triggering in the inverter legs creates better voltage profile and leads to the enabling of circulating current in the drive system. The induced circulating current flows through the apparatus neutral (N) and supply ground (G) is caused by the existence of parasitic capacitance. This circulating current may cause potential danger especially when parasitic capacitance poses large. In the past, different modulation techniques and conversion topologies have been introduced to minimize the flow of circulating current. However, these techniques lead to complexity, high cost, low voltage profile and efficiency due to lower modulation parameters. This paper proposes PS, POD, PD carrier shifting PWM algorithms for diode clamped MLI to tumbling the circulating current within the each phase of inverter legs. The performances of proposed algorithm, in terms of circulating current, THD, losses and efficiencies are analyzed theoreticallyand are validated via simulation and experimental results

Nitrogen ion beam synthesis of InN in InP (100) at elevated temperature

InN phase is grown in crystalline InP(100) substrates by 50 keV N+ implantation at an elevated temperature of 400 deg C followed by annealing at 525 deg C in N2 ambient. Crystallographic structural and Raman scattering studies are performed for the characterization of grown phases. Temperature- and power-dependent photoluminescence studies show direct band-to-band transition peak ~1.06 eV at temperatures <=150K. Implantations at an elevated temperature with a low ion beam current and subsequent low temperature annealing step are found responsible for the growth of high-quality InN phase.Comment: 11 pages, 4 figures, Journa

Dynamic Behavior of Geogrid-Reinforced Soil

Geosynthetic materials particularly geogrids are widely used as reinforcement and is a proven technology for enhancing the performance of the foundation soil system under monotonic loadings. But the dynamic behavior of the reinforced foundation soil has not been well understood so far, and therefore dynamic loads (square wave pulse) of frequencies 0.2, 0.4 and 1 hertz under an amplitude of displacement of 2mm have been studied by carrying out dynamic plate loading tests on both unreinforced and the reinforced sandy beds. The size of the square plate is 150mm x 150mm x 30mm

Promotion of Hendra virus replication by microRNA 146a

Hendra virus is a highly pathogenic zoonotic paramyxovirus in the genus Henipavirus. Thirty-nine outbreaks of Hendra virus have been reported since its initial identification in Queensland, Australia, resulting in seven human infections and four fatalities. Little is known about cellular host factors impacting Hendra virus replication. In this work, we demonstrate that Hendra virus makes use of a microRNA (miRNA) designated miR-146a, an NF-&kappa;B-responsive miRNA upregulated by several innate immune ligands, to favor its replication. miR-146a is elevated in the blood of ferrets and horses infected with Hendra virus and is upregulated by Hendra virus in human cells in vitro. Blocking miR-146a reduces Hendra virus replication in vitro, suggesting a role for this miRNA in Hendra virus replication. In silico analysis of miR-146a targets identified ring finger protein (RNF)11, a member of the A20 ubiquitin editing complex that negatively regulates NF-&kappa;B activity, as a novel component of Hendra virus replication. RNA interference-mediated silencing of RNF11 promotes Hendra virus replication in vitro, suggesting that increased NF-&kappa;B activity aids Hendra virus replication. Furthermore, overexpression of the I&kappa;B superrepressor inhibits Hendra virus replication. These studies are the first to demonstrate a host miRNA response to Hendra virus infection and suggest an important role for host miRNAs in Hendra virus disease

Pore resistivity variation by Resistivity imaging technique in sedimentary part of main Gadilam river basin, Cuddalore District, Tamil Nadu, India

Electrical resistivity is the only property of physics which give information of subsurface moisture content in the formation, Hence geophysical electrical resistivity survey was carried out to investigate the nature of shallow subsurface formations and geological contact in the main Gadilam river basin of Cuddalore District in Tamil Nadu. Twenty-seven vertical electrical soundings (VES) were conducted by Schlumberger configuration in the basin. Data is interpreted by curve matching techniques using IPI2 WIN software, layer parameters like apparent resistivity (?a) and thickness (h) interpretation were exported to Geographic Information System (GIS). Interpretation distinguishes three major geoelectric layers like topsoil, sandy clay layer, clayey sand layer along the contact zone in the basin. Interpreted VES sounding curves are mostly four-layer cases of QH, H, HA and KH type. Investigation demarcates lithology of subsurface and hydrogeological set up by employing maximum possible electrode sounding to infer saline water and freshwater occurrence based on resistivity signals. Zone of groundwater potential map was prepared with the combination of resistivity (?= ?1+ ?2+ ?3+ ?4) and corresponding thickness (T= T1+T2+T3+T4). High resistivity value of &gt;200 ? m and low resistivity value of &lt;10 ? m show the occurrence of alkaline and saline water within the formation aquifers as a result of possible rock water interaction and saline water dissolution. Four-layer resistivity cases from the matched curve (namely KH, AH, QA, and KA type) show the resistivity distribution/variation. It separates the freshwater depth wish from 1 to 140 ? m in fluvial sediments. Flood basin, sandstone and clay layer with low resistivity value of 3.16 - 7.5 ? m indicates contact with saline and freshwater aquifer. The Iso – resistivity map delineates saline water and freshwater zones with in the fourth layer cases in the same locations to indicate the irrational way of abstracting groundwater, resulting in saltwater ingress