Cracks in Martensite Plates as Hydrogen Traps in a Bearing Steel

It is demonstrated that a macroscopically homogeneous distribution of tiny cracks introduced into a martensitic bearing steel sample can provide powerful hydrogen traps. The phenomenon has been investigated through thermal desorption spectroscopy and hydrogen permeation measurements using both cracked and integral samples. The e↵ective hydrogen di↵usion coefficient through the cracked sample is found to be far less than in the uncracked one. Similarly, when samples are charged with hydrogen, and then subjected to thermal desorption analysis, the amount of hydrogen liberated from the cracked sample is smaller due to the trapping by the cracks. Theoretical analysis of the data shows that the traps due to cracks are so strong, that any hydrogen within the cracks can never in practice de-trap and cause harm by mechanisms that require the hydrogen to be mobile for the onset of embrittlement.W. Solano-Alvarez is very grateful for support from the Worshipful Company of Ironmongers, CONACyT, the Cambridge Overseas Trust, and the Roberto Rocca Education Programme.This is the accepted manuscript. The final publication is available at Springer via http://dx.doi.org/10.1007/s11661-014-2680-8

White-etching matter in bearing steel. Part II: Distinguishing cause and effect in bearing steel failure

The premature failure of large bearings of the type used in wind turbines, possibly through a mechanism called “white-structure flaking”, has triggered many studies of microstructural damage associated with “white-etching areas” created during rolling contact fatigue, although whether they are symptoms or causes of failure is less clear. Therefore, some special experiments have been conducted to prove that white-etching areas are the consequence, and not the cause, of damage. By artificially introducing a fine dispersion of microcracks in the steel through heat treatment and then subjecting the sample to rolling contact fatigue, manifestations of hard white-etching matter have been created to a much greater extent than samples similarly tested without initial cracks. A wide variety of characterization tools has been used to corroborate that the white areas thus created have the same properties as reported observations on real bearings. Evidence suggests that the formation mechanism of the white-etching regions involves the rubbing and beating of the free surfaces of cracks, debonded inclusions, and voids under repeated rolling contact. It follows that the focus in avoiding early failure should be in enhancing the toughness of the bearing steel in order to avoid the initial microscopic feature event.Funding by CONACyT, the Cambridge Overseas Trust, and the Roberto Rocca Education Programme is highly appreciated and acknowledged.This is the accepted manuscript version. The final published version is available from Springer at http://link.springer.com/article/10.1007%2Fs11661-014-2431-x

Spatiotemporal transcriptomic maps of whole mouse embryos at the onset of organogenesis

Spatiotemporal orchestration of gene expression is required for proper embryonic development. The use of single-cell technologies has begun to provide improved resolution of early regulatory dynamics, including detailed molecular definitions of most cell states during mouse embryogenesis. Here we used Slide-seq to build spatial transcriptomic maps of complete embryonic day (E) 8.5 and E9.0, and partial E9.5 embryos. To support their utility, we developed sc3D, a tool for reconstructing and exploring three-dimensional 'virtual embryos', which enables the quantitative investigation of regionalized gene expression patterns. Our measurements along the main embryonic axes of the developing neural tube revealed several previously unannotated genes with distinct spatial patterns. We also characterized the conflicting transcriptional identity of 'ectopic' neural tubes that emerge in Tbx6 mutant embryos. Taken together, we present an experimental and computational framework for the spatiotemporal investigation of whole embryonic structures and mutant phenotypes

Cell type-specific purifying selection of synonymous mitochondrial DNA variation

While somatic variants are well-characterized drivers of tumor evolution, their influence on cellular fitness in nonmalignant contexts remains understudied. We identified a mosaic synonymous variant (m.7076A > G) in the mitochondrial DNA (mtDNA)-encoded cytochrome c-oxidase subunit 1 (MT-CO1, p.Gly391=), present at homoplasmy in 47% of immune cells from a healthy donor. Single-cell multiomics revealed strong, lineage-specific selection against the m.7076G allele in CD8(+) effector memory T cells, but not other T cell subsets, mirroring patterns of purifying selection of pathogenic mtDNA alleles. The limited anticodon diversity of mitochondrial tRNAs forces m.7076G translation to rely on wobble pairing, unlike the Watson–Crick–Franklin pairing used for m.7076A. Mitochondrial ribosome profiling confirmed stalled translation of the m.7076G allele. Functional analyses demonstrated that the elevated translational and metabolic demands of short-lived effector T cells (SLECs) amplify dependence on MT-CO1, driving this selective pressure. These findings suggest that synonymous variants can alter codon syntax, impacting mitochondrial physiology in a cell type–specific manner