Variations of Lung Fissures: A Cadaveric Study

Background: The presence of fissures in the normal lungs enhances uniform expansion and hence facilitates more air intake. Accessory and incomplete fissures of varying depth can be seen in unusual locations of the lung, delimiting abnormal lobes which correspond to the normal bronchopulmonary segments. The knowledge of anatomical variations of lung fissures is essential for clinicians, surgeons, and for radiologist for recognizing various images of related abnormalities because an accessory or anomalous fissure can be mistaken for a lung lesion or an atypical appearance of pleural effusion. Aims and Objectives: The aim of the present study is to observe the variations of lung fissures in Indian population. Fifty pairs (right- 50; left- 50) of lungs were used for this study. Each lung was studied carefully for number of fissures whether complete or incomplete or absent. Presences of accessory fissures were noted. Results: We observed complete absence of fissures in two right and left lungs. Accessory fissures were present in 38% right lungs and 32% in left lungs. Conclusion: Incidence of absence of oblique fissure and accessory fissure was greater in our present work when compared our results with other authors. Considering this we feel that more elaborative study should be done on this topic which will throw more light on this

Identification and characterization of miRNAome in root, stem, leaf and tuber developmental stages of potato (Solanum tuberosum L.) by high-throughput sequencing

BACKGROUND: MicroRNAs (miRNAs) are ubiquitous components of endogenous plant transcriptome. miRNAs are small, single-stranded and ~21 nt long RNAs which regulate gene expression at the post-transcriptional level and are known to play essential roles in various aspects of plant development and growth. Previously, a number of miRNAs have been identified in potato through in silico analysis and deep sequencing approach. However, identification of miRNAs through deep sequencing approach was limited to a few tissue types and developmental stages. This study reports the identification and characterization of potato miRNAs in three different vegetative tissues and four stages of tuber development by high throughput sequencing. RESULTS: Small RNA libraries were constructed from leaf, stem, root and four early developmental stages of tuberization and subjected to deep sequencing, followed by bioinformatics analysis. A total of 89 conserved miRNAs (belonging to 33 families), 147 potato-specific miRNAs (with star sequence) and 112 candidate potato-specific miRNAs (without star sequence) were identified. The digital expression profiling based on TPM (Transcripts Per Million) and qRT-PCR analysis of conserved and potato-specific miRNAs revealed that some of the miRNAs showed tissue specific expression (leaf, stem and root) while a few demonstrated tuberization stage-specific expressions. Targets were predicted for identified conserved and potato-specific miRNAs, and predicted targets of four conserved miRNAs, miR160, miR164, miR172 and miR171, which are ARF16 (Auxin Response Factor 16), NAM (NO APICAL MERISTEM), RAP1 (Relative to APETALA2 1) and HAM (HAIRY MERISTEM) respectively, were experimentally validated using 5′ RLM-RACE (RNA ligase mediated rapid amplification of cDNA ends). Gene ontology (GO) analysis for potato-specific miRNAs was also performed to predict their potential biological functions. CONCLUSIONS: We report a comprehensive study of potato miRNAs at genome-wide level by high-throughput sequencing and demonstrate that these miRNAs have tissue and/or developmental stage-specific expression profile. Also, predicted targets of conserved miRNAs were experimentally confirmed for the first time in potato. Our findings indicate the existence of extensive and complex small RNA population in this crop and suggest their important role in pathways involved in diverse biological processes, including tuber development

Determination of some elemental content in Fabaceae family Cancer medicinal plants from North- East Karnataka region using SEM- EDX and FAAS spectrometry

The study aimed to determine the elemental content of Militia pinnata and Cassia tora Fabaceae family medicinal plants, traditionally used in cancer management and their medicinal properties. The analysis used Scanning microscope-Energy dispersive X-ray (SEM-EDX) and Flame atomic absorption (FAAS) techniques. The total 16 elemental content like the C, O, Mg Al, Si, Mo, K Sb, Ca Ti, Cr, Mn, Fe, Co, Ni and Zn in the plants sample. The results showed trace elements concentration in plants, with major are Mg, K and Ca and minor are Mn, Fe, Cu, Zn and Mo elements,  the concentration of Mg, K and Ca is highest, which is compared to  Mn, Fe, Cu, Zn and Mo  were determined in plants sample. These major and minor elements are within WHO ((World Health Organization) permissible limits

Reversible Image Watermarking Using Modified Quadratic Difference Expansion and Hybrid Optimization Technique.

With increasing copyright violation cases, watermarking of digital images is a very popular solution for securing online media content. Since some sensitive applications require image recovery after watermark extraction, reversible watermarking is widely preferred. This article introduces a Modified Quadratic Difference Expansion (MQDE) and fractal encryption-based reversible watermarking for securing the copyrights of images. First, fractal encryption is applied to watermarks using Tromino's L-shaped theorem to improve security. In addition, Cuckoo Search-Grey Wolf Optimization (CSGWO) is enforced on the cover image to optimize block allocation for inserting an encrypted watermark such that it greatly increases its invisibility. While the developed MQDE technique helps to improve coverage and visual quality, the novel data-driven distortion control unit ensures optimal performance. The suggested approach provides the highest level of protection when retrieving the secret image and original cover image without losing the essential information, apart from improving transparency and capacity without much tradeoff. The simulation results of this approach are superior to existing methods in terms of embedding capacity. With an average PSNR of 67 dB, the method shows good imperceptibility in comparison to other schemes

Reversible Image Watermarking Using Modified Quadratic Difference Expansion and Hybrid Optimization Technique

With increasing copyright violation cases, watermarking of digital images is a very popular solution for securing online media content. Since some sensitive applications require image recovery after watermark extraction, reversible watermarking is widely preferred. This article introduces a Modified Quadratic Difference Expansion (MQDE) and fractal encryption-based reversible watermarking for securing the copyrights of images. First, fractal encryption is applied to watermarks using Tromino's L-shaped theorem to improve security. In addition, Cuckoo Search-Grey Wolf Optimization (CSGWO) is enforced on the cover image to optimize block allocation for inserting an encrypted watermark such that it greatly increases its invisibility. While the developed MQDE technique helps to improve coverage and visual quality, the novel data-driven distortion control unit ensures optimal performance. The suggested approach provides the highest level of protection when retrieving the secret image and original cover image without losing the essential information, apart from improving transparency and capacity without much tradeoff. The simulation results of this approach are superior to existing methods in terms of embedding capacity. With an average PSNR of 67 dB, the method shows good imperceptibility in comparison to other schemes

CYTOTOXICITY OF STEM EXTRACTS OF SELECTED CASSIA SPECIES AGAINST HELA AND BREAST CANCER CELL LINES IN VITRO

ABSTRACTObjective: The use of plants for medicinal remedies is an integral part of the Indian cultural life, and the traditional background of Indian medicineshows widespread use of plant products in cancer treatment. In this study, stem extracts of some selected Cassia species have been evaluated for theircytotoxic activities under in vitro conditions.Methods: The stems were shade dried at room temperature. The dried and coarsely powdered plant material were extracted with petroleum ether(60-80°C), chloroform, and ethanol using soxhlet apparatus. The cytotoxicity was evaluated by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] assay.Results: Stem extracts of three Cassia species, viz., Cassia glauca, Cassia Obtusifolia, and Cassia sophera have been evaluated for their cytotoxic activitieswith chloroform, ethanol and pet ether against HeLa and breast cancer cell lines. Among the three different solvents used at different concentrations,the chloroform extracts of all Cassia species exhibited maximum cytotoxicity (%) against both cell lines. The CTC50 values are revealed the cytotoxicpotential of C. glauca chloroform extracts against HeLa cell line and breast cancer cell lines with CTC50 values 180.00±3.0 and 146.67±0.5, respectively.Chloroform extracts of C. Obtusifolia and C. sophera showed maximum activity against HeLa (380.00±1.1 and 800.00±1.7, respectively) and breastcancer cell lines (310.00±1.1 and 633.33±0.6).Conclusion: The results of this study demonstrate the potent cytotoxic activity of chloroform extracts of stems of Cassia species against HeLa andBreast cancer cell lines.Keywords: Cassia, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Cytotoxicity, HeLa, Breast cancer

Pharmaceutical analytical study of Hingwadi Taila

Hingwadi Taila is a Ayurvedic herbo mineral compound which is used mainly in the management of Kashtartava and Arthavajana. Hingwadi Taila contain drugs like Hingu, Kasisa, Saindhava, Kumari, Shunti, Tejapatra, Trikshara, Samudraphena, Haridra, Daruharidra and Sarshapa Taila. These drugs are very potent individually and possess Vedhana Sthapaka property. Our Acharyas has led down many pharmaceutical procedures through which many Dravyas can be taken in combination for better action and for easy assimilation to body. This Taila has got many Teekshna and Kshara Dravyas in it. Hence before administering internally, it has to be analyzed for its Organoleptic properties, acid value, Iodine value, moisture content w/w and saponification value. All the parameters are found to be good and within the standard parameters

Crop Security Model for Improvement in Agricultural Productivity Using Iot: Smart Farming

Most of the time in agriculture field, crops ravaged by local animals that leads to huge losses for the farmers. It’s very difficult for farmers to barricade entire fields and monitors continuously. Here the crop protection system model is developed for the farmers to prevent the crops from the animals. The model adopts the Arduino Uno based system and uses wired security that gives the shock to animals if they are approaching the field. The fire sensor is also used in the model to detect fire issues. In such situations, the microcontroller will turn ON the motor if there is a fire that interns intimate the farmers through mobile application. The temperature sensor and humidity sensors are also used in the model to provide the details of temperature and soil moisture of the field. The experimental values obtained by the model ensure complete safety of crops from animals and from fire thus protecting the farmer’s loss. In addition, mobile applications are also developed to provide the details of parameters such as temperature, moisture, water levels to farmers

Enhancement of Shelf-Life of Tomatoes Using Herbal Extracts

A study was taken up to enhance the shelf life of tomatoes by using Garlic (Allium sativam Linn) and Ginger (Zingiber officinale Rose) extracts at different concentrations viz, 1%, 5% and 10%. Tomatoes keeping quality was improved by spraying 10% garlic and ginger extracts, which reduced the spoilage, physiological loss and microbial load on the surface of the fruits. Among these, 10% garlic extract was more efficient in controlling spoilage microorganisms, enhancing shelf life and redicing physiological weight loss.   This study revealed the possibility of utilization of Garlic (Allium sativam Linn) and Ginger (Zingiber officinale Rose) extracts to enhance the keeping quality of tomatoes