Root morphology and seed and leaf ionomic traits in a Brassica napus L. diversity panel show wide phenotypic variation and are characteristic of crop habit

Background: Mineral nutrient uptake and utilisation by plants are controlled by many traits relating to root morphology, ion transport, sequestration and translocation. The aims of this study were to determine the phenotypic diversity in root morphology and leaf and seed mineral composition of a polyploid crop species, Brassica napus L., and how these traits relate to crop habit. Traits were quantified in a diversity panel of up to 387 genotypes: 163 winter, 127 spring, and seven semiwinter oilseed rape (OSR) habits, 35 swede, 15 winter fodder, and 40 exotic/unspecified habits. Root traits of 14 d old seedlings were measured in a ‘pouch and wick’ system (n = ~24 replicates per genotype). The mineral composition of 3–6 rosette-stage leaves, and mature seeds, was determined on compost-grown plants from a designed experiment (n = 5) by inductively coupled plasma-mass spectrometry (ICP-MS). Results: Seed size explained a large proportion of the variation in root length. Winter OSR and fodder habits had longer primary and lateral roots than spring OSR habits, with generally lower mineral concentrations. A comparison of the ratios of elements in leaf and seed parts revealed differences in translocation processes between crop habits, including those likely to be associated with crop-selection for OSR seeds with lower sulphur-containing glucosinolates. Combining root, leaf and seed traits in a discriminant analysis provided the most accurate characterisation of crop habit, illustrating the interdependence of plant tissues. Conclusions: High-throughput morphological and composition phenotyping reveals complex interrelationships between mineral acquisition and accumulation linked to genetic control within and between crop types (habits) in B. napus. Despite its recent genetic ancestry (<10 ky), root morphology, and leaf and seed composition traits could potentially be used in crop improvement, if suitable markers can be identified and if these correspond with suitable agronomy and quality traits

Impact of Abandoned Land on Environmental Protection, Landscape and Economic Benefit in Lithuania

Abstract. Human economic activity is defined as an anthropogenic process that has a negative impact on natural envi-ronment. The urbanisation and agricultural development influence the environment mostly. In order to receive economic benefit to the above-mentioned activities, the landscape is changed, the soil is affected and pollutants are released into the environment. In the light of the fact that the identified processes and problems caused by them are global, the variety of international directives are designed to minimise the impact of anthropogenic activities on the environment. The territory of the Republic of Lithuania has a considerable amount of areas, where the economic activity is sus-pended, i.e. the land is abandoned. This is due to a variety of social, natural and economic reasons. This process can be perceived as opposite to the anthropogenic activity, however it is important to examine how it affects the environment, landscape or economic needs. These processes are relevant on the local and international level, therefore, the scientific results of this article may be useful for the professionals in various areas and further research. The research investigates the territory of abandoned agricultural land, which is to be urbanised in the future. Land cadastre data, spatial planning documents are analysed, a questionnaire-based survey is conducted, and the actual inspec-tion in the area is carried out. The research results identify the causes for non-use of the land, and the impact of no eco-nomic activity on the environment, the landscape and the economic performance. To reach the set objectives, the data analysis, synthesis, induction methods have been used

Wpływ jesiennego terminu siewu na przezimowanie mieszańcowej i konwencjonalnej odmiany rzepaku ozimego

Field experiment on different sowing dates of two winter rapeseed cultivars (Brassica napus L. spp. and oleifera biennis Metzg.) was carried out in years 2009-2010 at the Experimental Station of the Aleksandras Stulginskis University (54°53’ N; 23°50’ E). The objective of the study was to evaluate the effect of winter rapeseed sowing date on the biometric parameters of rapeseed rosette: number of leaves, root collar diameter, height of apical bud, and its chemical composition: N:K ratio, amount of dry matter, total sugar in autumn before wintering, changes of chemical composition during the autumnwinter period, and overwintering. Sowing date significantly influenced all the examined indexes. According to the biometric parameters, the chemical composition of leaves, and the apical bud, winter rapeseed sown between August 30th and September 5th was best prepared for wintering and overwintered. Growth during the period of preparation for overwintering was more intensive. Changes in the chemical composition during the overwintering period were more favourable and the overwintering of winter rapeseed was better in the hybrid cultivar Kronos compared with cultivar Sunday.Doświadczenie polowe przeprowadzono w latach 2009-2010 w Stacji Doświadczalnej Litewskiego Uniwersytetu Rolniczego (54°53’ N; 23°50’ E). Zastosowano różne terminy siewu dla dwóch odmian rzepaku ozimego (Brassica napus L. spp. oleifera biennis Metzg.). Celem badań była ocena wpływu terminu siewu dwóch odmian rzepaku ozimego na wartość parametrów biometrycznych w stadium rozety, skład chemiczny jesienią przed przezimowaniem, zmiany składu chemicznego w okresie jesienno-zimowym i przezimowanie. Termin siewu znacząco wpłynął na wszystkie badane cechy. Według parametrów biometrycznych, składu chemicznego liści i pąków wierzchoł-kowych, rzepak ozimy zasiany w terminie od 30 sierpnia do 5 września był lepiej przygotowany do przezimowania i w konsekwencji przezimował najlepiej. Wzrost w okresie przygotowania do przezimowania był bardziej intensywny, zmiany składu chemicznego w ciągu zimowania były korzystniejsze oraz przezimowanie mieszańcowej odmiany rzepaku ozimego Kronos było lepsze niż tradycyjnej odmiany rzepaku ozimego Sunday

Controllable formation of high density SERS-active silver nanoprism layers on hybrid silica-APTES coatings

In this work sol-gel process for preparation of the uniform hybrid silica-3-aminopropyltriethoxysilane (APTES) coatings on glass surface is presented from mechanistic point of view. The suggested synthetic approach is straightforward, scalable and provides the means to tune the amount of amino groups on the surface simply by changing concentration of APTES in the initial sol. Deposition rate of different size silver nanoprisms (AgNPRs) on hybrid silica coatings of various amounts of APTES were studied and their performance as SERS materials were probed. The acquired data shows that the deposition rate of AgNPRs can be tuned by changing the amount of APTES. The optimal amount of APTES was found to be crucial for successful AgNPRs assembly and subsequent uniformity of the final SERS substrate—too high APTES content may result in rapid non-stable aggregation and non-uniform assembly process. SERS study revealed that SERS enhancement is the strongest at moderate AgNPRs aggregation level whereas it significantly drops at high aggregation levels