Genetic characterization of human coxsackievirus A6 variants associated with atypical hand, foot and mouth disease: a potential role of recombination in emergence and pathogenicity

Human coxsackievirus A6 (CVA6) is an enterically transmitted enterovirus. Until recently, CVA6 infections were considered as being of minor clinical significance, and only rarely aetiologically linked with hand, foot and mouth disease (HFMD) associated with other species A enteroviruses (particularly EV71 and CVA16). From 2008 onwards, however, CVA6 infections have been associated with several outbreaks worldwide of atypical HFMD (aHFMD) accompanied by a varicelliform rash. We recently reported CVA6-associated eczema herpeticum occurring predominantly in children and young adults in Edinburgh in January and February 2014. To investigate genetic determinants of novel clinical phenotypes of CVA6, we genetically characterized and analysed CVA6 variants associated with eczema herpeticum in Edinburgh in 2014 and those with aHFMD in CAV isolates collected from 2008. A total of eight recombinant forms (RFs) have circulated worldwide over the past 10 years, with the particularly recent appearance of RF-H associated with eczema herpeticum cases in Edinburgh in 2014. Comparison of phylogenies and divergence of complete genome sequences of CVA6 identified recombination breakpoints in 2A-2C, within VP3, and between 5' untranslated region and VP1. A Bayesian temporal reconstruction of CVA6 evolution since 2004 provided estimates of dates and the actual recombination events that generated more recently appearing recombination groups (RF-E, -F, -G and -H). Associations were observed between recombination groups and clinical presentations of herpangina, aHFMD and eczema herpeticum, but not with VP1 or other structural genes. These observations provided evidence that NS gene regions may potentially contribute to clinical phenotypes and outcomes of CVA6 infection

Upaya Isolasi β-Asarone pada Ekstrak N-heksan Rimpang Dringo (Acorus Calamus L.) Asal Kabupaten Pinrang

The isolation of n-hexane extract from Sweet Flag rhizome (Acorus calamus L.) was done. The aim of this research is to obtain β-asarone isolate as marker compound of Sweet Flag (Acorus calamus L.). Sample from 1 kg of dried rizhome, mascerate using n-hexane. This process produce 31 ml thick extract. Isolation of n-hexane extract using column chromatography with isocratic methode using n-hexane etil acetat 8 : 2. The isolate identified by UV-Vis spectroscopy, IR spectroscopy, (1H) NMR and Gas Chromatography Mass Spectro. From the data result show that isolate is β-asarone

Standarisasi Ekstrak Air Daun Jati Belanda dan Teh Hijau

Teh hijau memiliki nama spesies Camellia sinensis (L.) Kuntze, family Theaceae dan Jati belanda (Guazuma ulmifolia Lam.) termasuk kedalam family sterculiaceae Tujuan Penelitian ini adalah melakukan standarisasi ekstrak air daun jati belanda dan ekstrak air the hijau. Ekstrak distandardisasi dengan beberapa dua parameter yaitu parameter spesifik dan parameter non spesifik. Kadar sari larut air pada jati belanda 12,88 % dan teh hijau 40,88, sedangkan kadar sari larut etanol pada jati belanda 4, 23 % dan pada teh hijau 4,23 %. Hasil pengujian kandungan kimia menunjukkan pada ekstrak jati belanda mengandung saponin dan flavonoid sedangkan pada teh hijau mengandung tanin dan flavonoid. Kadar air ekstrak daun jati belanda 0,95 % dan teh hijau 2,79%. Hasil kadar abu total jati belanda sebesar 37,61% dan teh hijau 36,84%. Kadar abu tidak larut asam yaitu pada jati belanda sebesar 3,54% dan teh hijau 3,77%. Hasil dari penetapan susut pengeringan pada ekstrak jati belanda yaitu 0,46 % dan teh hijau 0,46 %. Ekstrak jati belanda maupun teh hijau berdasarkan pengujian standarisasi meliputi parameter spesifik dan non-spesifik memenuhi standarisasi mutu bahan baku

Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay

AbstractA robust oligonucleotide array-in-well hybridization assay using novel up-converting phosphor reporter technology was applied for genotyping clinically relevant human adenovirus types. A total of 231 adenovirus-positive respiratory, ocular swab, stool and other specimens from 219 patients collected between April 2010 and April 2011 were included in the study. After a real-time PCR amplification targeting the adenovirus hexon gene, the array-in-well assay identified the presence of B03 (n = 122; 57.5% of patients), E04 (29; 13.7%), C02 (21; 9.9%), D37 (14; 6.6%), C01 (12; 5.7%), C05 (5; 2.4%), D19 (4; 1.9%), C06 (2; 0.9%), D08 (1; 0.5%), A31 (1; 0.5%) and F41 (1; 0.5%) genotypes among the clinical sample panel. The typing result was obtained for all specimens that could be amplified (n = 223; 97%), and specificity of the typing was confirmed by sequencing specimens representing each of the different genotypes. No hybridization signal was obtained in adenovirus-negative specimens or specimens with other viruses (n = 30). The array-in-well hybridization assay has great potential as a rapid and multiplex platform for the typing of clinically relevant human adenovirus genotypes in different specimen types

Imidazopurinones are markers of physiological genomic damage linked to DNA instability and glyoxalase 1-associated tumour multidrug resistance

Glyoxal and methylglyoxal are reactive dicarbonyl metabolites formed and metabolized in physiological systems. Increased exposure to these dicarbonyls is linked to mutagenesis and cytotoxicity and enhanced dicarbonyl metabolism by overexpression of glyoxalase 1 is linked to tumour multidrug resistance in cancer chemotherapy. We report herein that glycation of DNA by glyoxal and methylglyoxal produces a quantitatively important class of nucleotide adduct in physiological systems—imidazopurinones. The adduct derived from methylglyoxal-3-(2′-deoxyribosyl)-6,7-dihydro-6,7-dihydroxy-6/7-methylimidazo-[2,3-b]purine-9(8)one isomers—was the major quantitative adduct detected in mononuclear leukocytes in vivo and tumour cell lines in vitro. It was linked to frequency of DNA strand breaks and increased markedly during apoptosis induced by a cell permeable glyoxalase 1 inhibitor. Unexpectedly, the DNA content of methylglyoxal-derived imidazopurinone and oxidative marker 7,8-dihydro-8-oxo-2′-deoxyguanosine were increased moderately in glyoxalase 1-linked multidrug resistant tumour cell lines. Together these findings suggest that imidazopurinones are a major type of endogenous DNA damage and glyoxalase 1 overexpression in tumour cells strives to counter increased imidazopurinone formation in tumour cells likely linked to their high glycolytic activity

Life style related risk factors for cardiovascular disease among patients at a teaching hospital in Karachi

BACKGROUND: Cardiovascular disease is a leading cause for morbidity and mortality all over the world. It is important to study life style related, modifiable cardiovascular risk factors among patients, in order to devise preventive strategies. METHODS: We surveyed family practice patients visiting the out-patient clinics of Aga Khan University Hospital, Karachi. The interview was questionnaire-based and recorded the demographic profile of the patients, in addition to information on life style related cardiovascular risk factors. The ethical requirements for the study were met. SPSS computer software was used for data management. RESULTS: Fifty patients were surveyed and included 28 (56%) men and 22 (44%) women. Thirty seven (74%) respondents were married, nineteen (38%) had graduate education, twenty five (50%) were in private service, and twelve (24%) were housewives. Twenty six (52%) respondents were overweight or obese. Butter, cream, margarine and red meat was consumed by 17 (34%) and 32 (64%) respondents respectively. White meat, fresh fruits and vegetables were consumed by 30 (60%), 29 (58%) and 38 (76%) respondents respectively. Oil from vegetable sources was used by 49 (98%) respondents. Addition of table salt was found occasionally and always in 18 (36%) and 09 (18%) respondents respectively. Twenty seven (54%) respondents exercised at least twenty minutes, three times a week, while eleven (22%) were current smokers. CONCLUSIONS: Life style related modifiable risk factors are prevalent in patients reporting to our hospital. This offers an opportunity for preventive strategies, to prevent cardiovascular disease. Such surveys, followed by interventional strategies, are recommended to be followed by all Primary care facilities

ANALISIS PEMASARAN SAPI MADURA DI PASAR BENGKAL KABUPATEN SUMENEP

Penelitian dilaksanakan pada tanggal 13 September sampai 26 September 2021. Penelitian ini bertujuan untuk mengetahui Kelayakan Finansial Usaha Peternakan Sapi Potong di Kecamatan Tujuh Belas Kabupaten Bengkayang Provinsi Kalimantan Barat. Materi yang dipakai dalam penelitian ini adalah 10  peternak kuesioner responden. Metode yang digunakan dalam penelitian ini adalah metode penelitian survey dengan mengumpulkan data angket (kuesioner) dan wawancara dengan pengambilan secara purposive sampling. Hasil penelitian menunjukan nilai rata-rata biaya tetap sebesar Rp. 2.418.000, rata-rata biaya variabel sebesar Rp.159.725.900, rata-rata biaya total sebsar Rp.161.761.900, rata-rata analisis penerimaan sebesar Rp.266.630.000, rata rata pendapatan sebesar Rp. 104.888.100, nilai R/C Ratio sebesar 1,64, sedang nilai BEP produksi sebesar 6 ekor dan BEP harga sebesar Rp. 17.971.322 yang artinya bahwa usaha tersebut layak diusahakan dengan minimal beternak sebanyak 6 ekor dengan harga jual Rp.17.971.322 /ekor.Kata kunci:saluran pemasaran Sapi Madura,margin pemasaran,efisiensi pemasaran

Implications for the practice of a patient expectation and satisfaction survey, at a teaching hospital in Karachi, Pakistan

Objective: To study the expectations and satisfaction of patients visiting Family Practice Clinics, at the Aga Khan University Hospital, in Karachi, Pakistan. Methodology: A cross sectional survey of 316 patients was carried out. A questionnaire, based on the study objectives was developed and administered. The participating patient signed a consent form, after assurance of confidentiality was provided. Data on the demographic profile of the patients was collected.Results: The mean age was 33.81 years, with 105 (33.2%) women, and 211 (66.8%) men. The majority were married, with education above intermediate level and were in private or government service or were housewives. The median for the patient waiting time was 30 minutes, against an expectation of 12.69 minutes. Reading newspaper, watching television, reading magazine, reading Quran and listening to music were quoted as ways to lessen the burden of waiting to see a physician. The average consultation time with the physician was 13.89 minutes, against an expectation of 16.37 minutes. Patient expectation in terms of listening by the doctor with patience, explanation of the diagnosis and treatment, prescription of medicines, ordering of investigations and specialist referral has been documented. Objections to the presence of medical student, nursing student, resident doctor, nurse and an observer, in the consultation room have also been documented. Reasons quoted for the objection include issues of privacy/confidentiality, lack of justification, discomfort, and interference with the consultation process. The expected average cost for doctor’s consultation was Pakistani Rs. 124, while 196 (61.8%) of the respondents were satisfied with the consultation based on Rs. 70. Conclusion: We have collected important information to improve the services offered at our Family Practice clinics (JPMA 53:122;2003)