83 research outputs found
Effects of myosin and heavy meromyosin on actin-related gelation of HeLa cell extracts.
Comparison of 3D transitional CFD simulations for rotating wind turbine wings with measurements:Paper
Since the investigation of van Ingen et al., attempts were undertaken to search for laminar parts within the boundary layer of wind turbines operating in the lower atmosphere with much higher turbulence levels than seen in wind tunnels or at higher altitudes where airplanes usually fly. Based on the results of the DAN-Aero experiment and the Aerodynamic Glove project, a special work package Boundary Layer Transition was embedded in IAEwind Task 29 MexNext 3rd phase (MN3). Here, we report on the results of the application of various CFD tools to predict transition on the MEXICO blade. In addition, recent results from a comparison of thermographic pictures (aimed at detecting transition) with 3D transitional CFD are included as well. The MEXICO (2006) and NEW MEXICO (2014) wind tunnel experiments on a turbine equipped with three 2.5 m blades have been described extensively in the literature. In addition, during MN3, high-frequency Kulite data from experiments were used to detect traces of transitional effects. Complementary, the following set of codes were applied to cases 1.1 and 1.2 (axial inflow with 10 m/s and 15 m/s respectively) – elsA, CFX, OpenFOAM (with 2 different turbulence/transitional models), Ellipsys, (with 2 different turbulence models and eN transition prediction tool), FLOWer and TAU – to search for detection of laminar parts by means of simulation. Obviously, the flow around a rotating blade is much more complicated than around a simple 2D section. Therefore, results for even integrated quantities like thrust and torque are varying strongly. Nevertheless, visible differences between fully turbulent and transitional set-ups are present. We discuss our findings, especially with respect to turbulence and transition models used
A hybrid actuator disc - full rotor CFD methodology for modelling the effects of wind turbine wake interactions on performance
The performance of individual wind turbines is crucial for maximum energy yield, however, their performance is often reduced when turbines are placed together in an array. The wake produced by the rotors interacts with downstream turbines, resulting in a reduction in power output. In this paper, we demonstrate a new and faster modelling technique which combines actuator disc theory, modelled using wind tunnel validated Computational Fluid Dynamics (CFD), and integrated into full rotor CFD simulations. This novel hybrid of techniques results in the ability to analyse performance when simulating various array layouts more rapidly and accurately than using either method on its own. It is shown that there is a significant power reduction from a downstream turbine that is subjected to the wake of an upstream turbine, and that this is due to both a reduction in power in the wind and also due to changes in the aerodynamics. Analysis of static pressure along the blade showed that as a result of wake interactions, a large reduction in the suction peak along the leading edge reduced the lift generated by the rotor and so reduced the torque production and the ability for the blade to extract energy from the wind
Date of Planting Studies of Winter Wheat and Winter Barley in Relation to Root and Crown Rot Grain Yields and Quality
This study was undertaken to determine the nature of root and crown rot of wheat and to ascertain the proper time to plant winter wheat so as to conserve moisture, minimize root and crown rot and thereby maximize yield. Studies conducted for 15 years at several locations in western Nebraska reveal that planting date is a significant factor for higher yields, particularly in certain years
Microtubule-associated proteins of HeLa cells: heat stability of the 200,000 mol wt HeLa MAPs and detection of the presence of MAP-2 in HeLa cell extracts and cycled microtubules.
Results of the Cooperative Uniform Soybean Tests, 1947 Part I. North Central States
United States Department of Agriculture, Agricultural Research Administration; Bureau of Plant Industry, Soils, and Agricultural Engineering, Division of Forage Crops and Diseases Cooperating with State Agricultural Experiment Station
Results of the Cooperative Uniform Soybean Tests, 1946 Part I. North Central States
United States Department of Agriculture, Agricultural Research Administration; Bureau of Plant Industry, Soils, and Agricultural Engineering, Division of Forage Crops and Diseases Cooperating with State Agricultural Experiment Station
Structural and Functional Evaluation of C. elegans Filamins FLN-1 and FLN-2
Filamins are long, flexible, multi-domain proteins composed of an N-terminal actin-binding domain (ABD) followed by multiple immunoglobulin-like repeats (IgFLN). They function to organize and maintain the actin cytoskeleton, to provide scaffolds for signaling components, and to act as mechanical force sensors. In this study, we used transcript sequencing and homology modeling to characterize the gene and protein structures of the C. elegans filamin orthologs fln-1 and fln-2. Our results reveal that C. elegans FLN-1 is well conserved at the sequence level to vertebrate filamins, particularly in the ABD and several key IgFLN repeats. Both FLN-1 and the more divergent FLN-2 colocalize with actin in vivo. FLN-2 is poorly conserved, with at least 23 IgFLN repeats interrupted by large regions that appear to be nematode-specific. Our results indicate that many of the key features of vertebrate filamins are preserved in C. elegans FLN-1 and FLN-2, and suggest the nematode may be a very useful model system for further study of filamin function
The filamins: properties and functions
The filamins are a group of homologous proteins defined by their high native molecular weight (500 000), their amino acid compositions, their cross-reactivity to antibodies to heterologous filamins, their localization to actin networks and bundles in situ, and their ability to cross-link actin filaments in vitro into three-dimensional networks and bundles. Native filamins contain two subunits (relative mass = 250 000). Each subunit carries at least one actin-binding site and formation of bivalent dimers is therefore believed to explain filamin's ability to cross-link actin filaments. Formation of networks in vitro (corresponding to formation of macroscopic gels) has been analyzed using the theory of Flory. As predicted, a sharp transition to gel (at the critical gelation concentration of filamin) is observed when actin is mixed with increasing concentrations of filamin and the critical gelation concentration is found to vary inversely with the length of actin filaments. However, the measured values of the critical gelation concentration are all higher (2- to 14-fold) than predicted by the theory and the prediction that the critical concentration varies directly with the actin concentration was verified with only one of two techniques used. Filamin's length (160–190 nm) and flexibility (1000-fold greater than actin filaments) may make it especially well fitted to cross-link actin filaments into three-dimensional networks when present in low molar ratios (1:200 to 1:50) relative to actin. At higher molar ratios (> 1:20) it also cross-links actin filaments into bundles. Assuming that filamin actually helps organize supramolecular structures inside cells (not yet tested directly), then its concentration relative to actin may help determine whether networks or bundles are formed. Other factors that may influence its localization and function inside cells include competition with other actin-binding proteins (such as myosin and tropomyosin) for binding sites on actin and phosphorylation, which may alter its ability to bind to actin. </jats:p
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