X-Ray Microanalysis in Cryosections of Natively Frozen Paramecium Caudatum with Regard to Ion Distribution in Ciliates

Cells of Paramecium caudatum were shock-frozen without pretreatment for cryoultramicrotomy and freeze-dried for subsequent X-ray microanalysis. Na, Mg, P, S, Cl, K, and Ca were detected in different amounts in several subcellular compartments. In particular, calcium was localized below the cell surface (pellicle). Trichocysts were found to contain significant amounts of Na in their base but not in the tip. Na, Mg, P, S, Cl, K, Ca were found in electron dense deposits within the lumen of the contractile vacuole. A small K concentration was found in the cytoplasm and in the mitochondria. X-ray microanalysis of the element distribution in different subcellular compartments provides information for the understanding of cellular functions such as exocytosis, locomotion, and ion regulation

The Influence of Different Cryopreparations on the Distribution of Ions in Bullfrog Myocard Cells

Bullfrog heart muscle trabecula are shock-frozen in liquid propane cooled by liquid nitrogen and then processed for X-ray microanalysis in two different ways: 1. Freeze-drying followed by vacuum embedding. 2. Cryoultramicrotomy and freeze-drying. Stained sections of freeze-dried, embedded tissue exhibit detailed ultrastructure, but are useless for X-ray microanalysis. Unstained, dry cut plastic-sections are suitable for X-ray microanalysis, but the ultrastructure appears faint. Higher electron optical contrast and peak-to-background ratio of X-ray spectra are generally obtained in freeze-dried cryosections. Both preparation methods show that the X-ray spectra are influenced by the quality of cryofixation. The phosphorus/potassium ratio in nuclei increases with increasing ice crystal size

Identification of Bone Marrow Cell Subpopulations Associated With Improved Functional Outcomes in Patients With Chronic Left Ventricular Dysfunction: An Embedded Cohort Evaluation of the FOCUS-CCTRN Trial

In the current study, we sought to identify bone marrow-derived mononuclear cell (BM-MNC) subpopulations associated with a combined improvement in left ventricular ejection fraction (LVEF), left ventricular end-systolic volume (LVESV), and maximal oxygen consumption (VO2 max) in patients with chronic ischemic cardiomyopathy 6 months after receiving transendocardial injections of autologous BM-MNCs or placebo. For this prospectively planned analysis, we conducted an embedded cohort study comprising 78 patients from the FOCUS-Cardiovascular Cell Therapy Research Network (CCTRN) trial. Baseline BM-MNC immunophenotypes and progenitor cell activity were determined by flow cytometry and colony-forming assays, respectively. Previously stable patients who demonstrated improvement in LVEF, LVESV, and VO2 max during the 6-month course of the FOCUS-CCTRN study (group 1, n = 17) were compared to those who showed no change or worsened in one to three of these endpoints (group 2, n = 61) and to a subset of patients from group 2 who declined in all three functional endpoints (group 2A, n = 11). Group 1 had higher frequencies of B-cell and CXCR4(+) BM-MNC subpopulations at study baseline than group 2 or 2A. Furthermore, patients in group 1 had fewer endothelial colony-forming cells and monocytes/macrophages in their bone marrow than those in group 2A. To our knowledge, this is the first study to show that in patients with ischemic cardiomyopathy, certain bone marrow-derived cell subsets are associated with improvement in LVEF, LVESV, and VO2 max at 6 months. These results suggest that the presence of both progenitor and immune cell populations in the bone marrow may influence the natural history of chronic ischemic cardiomyopathy-even in stable patients. Thus, it may be important to consider the bone marrow composition and associated regenerative capacity of patients when assigning them to treatment groups and evaluating the results of cell therapy trials

Electrical and Thermal Transport at the Planckian Bound of Dissipation in the Hydrodynamic Electron Fluid of WP2

Materials with strongly-correlated electrons exhibit interesting phenomena such as metal-insulator transitions and high-temperature superconductivity. In stark contrast to ordinary metals, electron transport in these materials is thought to resemble the flow of viscous fluids. Despite their differences, it is predicted that transport in both, conventional and correlated materials, is fundamentally limited by the uncertainty principle applied to energy dissipation. Here we discover hydrodynamic electron flow in the Weyl-semimetal tungsten phosphide (WP2). Using thermal and magneto-electric transport experiments, we observe the transition from a conventional metallic state, at higher temperatures, to a hydrodynamic electron fluid below 20 K. The hydrodynamic regime is characterized by a viscosity-induced dependence of the electrical resistivity on the square of the channel width, and by the observation of a strong violation of the Wiedemann-Franz law. From magneto-hydrodynamic experiments and complementary Hall measurements, the relaxation times for momentum and thermal energy dissipating processes are extracted. Following the uncertainty principle, both are limited by the Planckian bound of dissipation, independent of the underlying transport regime

Clinical Performance of the Automated LIAISON® Meridian H. pylori SA Stool Antigen Test

Background. Antigens derived from Helicobacter pylori can be used as stool biomarkers to assist in the diagnosis of H. pylori infection. Since current assays have variable performance, we assessed the clinical performance of the automated LIAISON® Meridian H. pylori SA chemiluminescent immunoassay against more invasive biopsy tests that are considered to be the "gold standard" (Composite Reference Method). Methods. This prospective multisite study enrolled patients undergoing an esophagogastroduodenoscopy with collection of biopsy and stool specimens. Adult patients (≥22 years) participated in the study from February 2017 to August 2018. Specimens of the stomach were tested by three methods, known as the Composite Reference Method: (1) histological evaluation, (2) culture of the organism, and (3) rapid urease detection test. H. pylori in stool was detected using the automated LIAISON® Meridian H. pylori SA assay, a chemiluminescent immunoassay. Statistical analyses were performed using MedCalc 18.11.6. Results. 277 patients (63% female) were included in the study. The prevalence of infected subjects was 24.2% in this study cohort. Clinical performance assessed against the Composite Reference Method showed very good agreement (Cohen's kappa=0.922), with good sensitivity (95.5%) and specificity (97.6%). Reproducibility study results showed total imprecision ranging from 3.1% to 13.9% CV. Conclusion. The automated LIAISON® Meridian H. pylori SA assay brings reliable noninvasive testing for H. pylori to the laboratory that is in very good agreement with the current, more invasive biopsy-based methods such as histology, culture, or rapid urease test. The clinical trial identifiers are NCT03060746 (pretherapy) and NCT03060733 (posttherapy)

Impact of surface treatments on the photocatalytic performance of anodic aluminum oxide templates

Nanostructured materials receive great interest nowadays due to their unique properties, increased surface areas, and superior performances with associated reduced material usage. However, directly nanostructuring functional materials themselves can be technologically challenging. Hence, nanostructured substrates such as anodic aluminum oxide (AAO) can serve as templates for depositing active materials. The templates’ chemical stability is crucial for accurately assessing functional materials. Since AAO structures incorporate electrolyte ions during anodization, their chemical stability is influenced by the subsequent processing after anodization. This work investigates the effect of various post-anodization modifications on the surface chemistry and photocatalytic performance of bare AAO structures. Treatments with H2O2 or H3PO4 can stabilize the photocatalytic performance of the AAO templates over consecutive measurements. XPS measurements indicate that such stabilization results from AAO surface chemistry alterations. Further, we explore the functionalization of these modified AAO templates with photocatalysts by atomic layer deposition. The photocatalytic performance of TiO2 as a chemically stable photocatalyst is not affected by the templates’ post-anodization treatment. In contrast, the performance of templates functionalized with Fe2O3 as an inherent chemically instable photocatalyst depends on the template stability. This work highlights the importance of chemically stable template materials for exploring the properties of new functional materials

Enhancing the Photocatalytic Activity by Tailoring an Anodic Aluminum Oxide Photonic Crystal to the Semiconductor Catalyst: At the Example of Iron Oxide

Photonic crystals (PhCs) are interesting structures for photocatalytic applications because of their capability of harnessing distinct forms of light–matter interactions within the PhCs. Of all these, overlapping one of the photonic stopband's (PSB) edge with the absorption of the PhC material or adsorbed molecules improves their excitation and generated charge carriers can subsequently induce photocatalytic reactions. The PSB position of anodic aluminum oxide PhCs (AAO-PhCs) can be easily adjusted by modifying the anodization profile. Herein, AAO-PhCs are designed to match the band gap of a model semiconductor enabling a general photocatalytic activity enhancement independent of the chemical to be decomposed. Fe2O3, as an example photocatalyst, is coated onto AAO-PhCs to demonstrate efficient photocatalytic systems by utilizing the slow photon effect. Tailored Fe2O3-AAO-PhCs with their PSB edge at 564 nm matching the Fe2O3 band gap exhibit generally enhanced degradation of three different organic dyes while a significant activity decrease is observed when the PSB edge does not overlap with the Fe2O3 absorption. Furthermore, photocatalyst degradation can be reduced down to only 4% activity loss over six consecutive measurements by an ultra-thin alumina coating.Authors would like to acknowledge funding by the Deutsche Forschungsgemeinschaft (DFG) within the Collaborative Research Initiative SFB 986 “Tailor-Made Multi-Scale Materials Systems” (project number 192346071). Spanish MCINN and AEI under research grant No. PID2019-108075RB-C32/AEI/10.13039/501100011033 are also gratefully acknowledged. Authors thank the support provided by the Australian Research Council through the grants DP200102614 and DP220102857. The authors acknowledge financial support from the Open Access Publication Fund of the Universität Hamburg

Enhancing the Photocatalytic Activity by Tailoring an Anodic Aluminum Oxide Photonic Crystal to the Semiconductor Catalyst: At the Example of Iron Oxide

Published online: OnlinePublPhotonic crystals (PhCs) are interesting structures for photocatalytic applications because of their capability of harnessing distinct forms of light–matter interactions within the PhCs. Of all these, overlapping one of the photonic stopband’s (PSB) edge with the absorption of the PhC material or adsorbed molecules improves their excitation and generated charge carriers can subsequently induce photocatalytic reactions. The PSB position of anodic aluminum oxide PhCs (AAO-PhCs) can be easily adjusted by modifying the anodization profile. Herein, AAO-PhCs are designed to match the band gap of a model semiconductor enabling a general photocatalytic activity enhancement independent of the chemical to be decomposed. Fe₂O₃, as an example photocatalyst, is coated onto AAO-PhCs to demonstrate efficient photocatalytic systems by utilizing the slow photon effect. Tailored Fe₂O₃-AAO-PhCs with their PSB edge at 564 nm matching the Fe₂O₃ band gap exhibit generally enhanced degradation of three different organic dyes while a significant activity decrease is observed when the PSB edge does not overlap with the Fe₂O₃ absorption. Furthermore, photocatalyst degradation can be reduced down to only 4% activity loss over six consecutive measurements by an ultra-thin alumina coating.Carina Hedrich, Anna R. Burson, Silvia González-García, Víctor Vega, Victor M. Prida, Abel Santos, Robert H. Blick, and Robert Zierol

Electrochemical engineering of nanoporous materials for photocatalysis: fundamentals, advances, and perspectives

Photocatalysis comprises a variety of light-driven processes in which solar energy is converted into green chemical energy to drive reactions such as water splitting for hydrogen energy generation, degradation of environmental pollutants, CO₂ reduction and NH3 production. Electrochemically engineered nanoporous materials are attractive photocatalyst platforms for a plethora of applications due to their large effective surface area, highly controllable and tuneable light-harvesting capabilities, efficient charge carrier separation and enhanced diffusion of reactive species. Such tailor-made nanoporous substrates with rational chemical and structural designs provide new exciting opportunities to develop advanced optical semiconductor structures capable of performing precise and versatile control over light–matter interactions to harness electromagnetic waves with unprecedented high efficiency and selectivity for photocatalysis. This review introduces fundamental developments and recent advances of electrochemically engineered nanoporous materials and their application as platforms for photocatalysis, with a final prospective outlook about this dynamic field.Siew Yee Lim, Cheryl Suwen Law, Lina Liu, Marijana Markovic, Carina Hedrich, Robert H. Blick, Andrew D. Abell, Robert Zierold, and Abel Santo

High Aspect Ratio Nanotubes fabricated by Ion-Track Technology and Atomic Layer Deposition

Nanotubes and nanochannels embedded in solid state membranes are of high relevance in many different fields including nanofluidics, catalysis, health care, or solar energy harvesting. On the way to novel industrial applications, systematic basic studies on these nanostructures as well as development of suitable fabrication techniques to precisely tailor their dimensions and surface properties are required. For synthesis of cylindrical nanochannels anodic alumina and polymer membranes are frequently used