Dermatophilosis in a buffalo: A case report

Dermatophilosis is caused by Dermatophilus Congolensis, pandemic  but more prevalent in tropical countries. The present paper reports the successful diagnosis and treatment of dermatophilosis in a graded Murrah buffalo heifer. Three year-old graded Murrah heifer and weighing approximately 200 kg was referred to teaching veterinary clinical complex with the history of inappetence, pruritus, dirty yellow colored scabs and crusts all over the body. The scabs and crusts were collected for direct microscopy with the presence of filamentous and branching zoospores in the impression smears confirmed the dermatophilosis. The animal was treated initially with Oxytetracycline 10 mg/kg body weight intravenously along with intramuscular Chlorpheniramine maleate 0.2 mg/kg for first five days and it was followed by long acting Oxytetracycline 20 mg/kg through intramuscular route. The animal was showed marked improvement after 9 days of therapy. Complete clinical improvement was noticed after 16 days of therapy with the disappearance of scabs and crusts

ANTIOXIDANT, FREE RADICAL SCAVENGING ACTIVITY AND GC-MS STUDIES ON PEDILANTHUS TITHYMALOIDES (L.) POIT

Objective: To evaluate the methanolic extract of the leaves of Pedilanthus tithymaloides for total phenol, total flavonoid, total antioxidant and free radical scavenging ability and detect the phytoconstituents using GC-MS. Methods: The total phenols were quantified using Folin-Ciocalteu method. Aluminium chloride method and Phosphomolybdenum method were used to quantify total flavonoid and total antioxidant contentrespectively. In addition to the above, Ferric thiocyanate assay, the thiobarbituric acid assay,Ferric Reducing Antioxidant Power assay and ABTS assay were performed to know the antioxidant potency of the methanolic extract of leaves of Pedilanthus tithymaloides. The phytoconstituents was detected using GC-MS. Results: The leaves of Pedilanthus tithymaloides recorded a phenolic content of 10.98±0.08 mg TAE/g DW, flavonoid content of 11.49±0.15 µg QE/g DW and total antioxidant content of 6.64±0.05 mg TAE/g DW. The study also revealed significant free radical scavenging ability of the plant leaves as assessed by FTC, TBA, FRAP and ABTS assays. The structural elucidation by GC-MS analysis revealed five different compounds, includingthree esters, an amine and an alkaloid. Conclusion: The study proves the anticipative potential ability of Pedilanthus tithymaloides, suggesting its exploitation in pharmaceutical applications

4′-(2,4-Dichloro­phen­yl)-1,1′-dimethyl­piperidine-3-spiro-3′-pyrrolidine-2′-spiro-3′′-indoline-4,2′′-dione

In the title compound, C23H23Cl2N3O2, the pyrroline ring adopts an envelope conformation and the piperidinone ring assumes a slightly twisted chair form. In the crystal, inversion dimers linked by pairs of N—H⋯O hydrogen bonds generate an R 2 8 graph-set motif and a short Cl⋯Cl contact of 3.478 (1) Å occurs

Antibacterial Effect of Green Synthesized Silver Nanoparticles using Cineraria maritima

Nanoparticles display entirely novel physicochemical characteristics for specific applications because of their exceptional size and shape. Owing to the present study, we reported biosynthesis, characterization and antibacterial properties of Cineraria maritima (Cm) assisted silver nanoparticles (Ag NPs). The surface plasmon vibration, crystalline structure, surface morphology, elemental composition, and possible functional molecules vibration of prepared Cm-Ag NPs were characterized by different instrumentation techniques. The spectrum of UV-Vis of Cm-Ag NPs showed maximum plasma intensity occurred around 425nm. XRD spectrum showed the face-centred cubic (FCC) nature of Cm-Ag NPs. The SEM image of the Cm-Ag NPs demonstrated a predominantly spherical shape with cluster formation of small particles to large particles with sizes ranging from 21.57 nm to 39.16 nm. EDS spectrum indicated the existence of Ag elements in Cm-Ag NPs. FTIR intense peaks of Cm-Ag NPs showed the different functional molecules such as phenol, alkene, aldehydes, and a carbonyl group. In addition, Cm-Ag NPs coated textile cotton fabric sample showed substantial anti-bacterial properties against a tested bacterial pathogen

Synthesis, Characterization and Remedial Action of Biogenic Silver Nanoparticles and Chitosan-Silver Nanoparticles against Bacterial Pathogens

Custard apple is a dry land fruit. Its leaves exhibit different pharmacological activities. In the present study, both silver (Ag) nanoparticles and chitosan-coated Ag (Chi-Ag) nanoparticles were fabricated using the aqueous leaf extract of the custard apple plant. During preliminary phytochemical analysis, various types of phytocompounds were found in the aqueous leaf extract of the same plant. Next, both nanoparticles were physiochemically characterized. FTIR analysis exhibited the fingerprint vibrational peaks of active bioactive compounds in plant extract, Ag nanoparticles, and Chi-Ag nanoparticles. UV/Visible spectral analysis revealed the highest absorbance peak at 419 nm, indicating the presence of Ag nanoparticles. XRD analysis presented the face-centered cubic (FCC) structure of both prepared nanomaterials. Further, the average crystalline size of both Ag nanoparticles and Chi-Ag nanoparticles was calculated to be 23 and 74 nm, respectively. FESEM analysis showed the spherical and cubical shapes of Ag nanoparticles and Chi-Ag nanoparticles, respectively. EDS analysis indicated a peak around 3.29 keV, conforming to the binding energies of Ag ions. The biogenic nanomaterial also showed strong antibacterial activity against all tested bacterial pathogens.</p

Early life drought predicts components of adult body size in wild female baboons

Objectives: In many taxa, adverse early-life environments are associated with reduced growth and smaller body size in adulthood. However, in wild primates, we know very little about whether, where, and to what degree trajectories are influenced by early adversity, or which types of early adversity matter most. Here, we use parallel-laser photogrammetry to assess inter-individual predictors of three measures of body size (leg length, forearm length, and shoulder-rump length) in a population of wild female baboons studied since birth. Materials and Methods: Using >2000 photogrammetric measurements of 127 females, we present a cross-sectional growth curve of wild female baboons (Papio cynocephalus) from juvenescence through adulthood. We then test whether females exposed to several important sources of early-life adversity—drought, maternal loss, low maternal rank, or a cumulative measure of adversity—were smaller for their age than females who experienced less adversity. Using the “animal model,” we also test whether body size is heritable in this study population. Results: Prolonged early-life drought predicted shorter limbs but not shorter torsos (i.e., shoulder-rump lengths). Our other measures of early-life adversity did not predict variation in body size. Heritability estimates for body size measures were 36%–67%. Maternal effects accounted for 13%–17% of the variance in leg and forearm length, but no variance in torso length. Discussion: Our results suggest that baboon limbs, but not torsos, grow plastically in response to maternal effects and energetic early-life stress. Our results also reveal considerable heritability for all three body size measures in this study population

High Performance Liquid Chromatographic Fluorescence Detection Method for the Quantification of Rivastigmine in Rat Plasma and Brain: Application to Preclinical Pharmacokinetic Studies in Rats

A highly sensitive and selective high performance liquid chromatographic fluorescence detection method has been developed and validated for the quantification of rivastigmine in rat plasma and brain. Protein precipitation and one-step liquid–liquid extraction techniques were utilized for the extraction of RSM from brain and plasma, respectively, along with an internal standard. The chromatographic separation was achieved with a column inertsil ODS-3V and a mobile phase consisting of ammonium acetate buffer (20 mM, pH 4.5) and acetonitrile (76:24, v/v) delivered at a flow rate of 1 ml/min. The lower limit of quantitation for the developed method was 10 ng/mL for both matrices. The method was found to be accurate and reproducible and was successfully used to quantify levels of RSM in plasma and brain following intravenous administration of RSM in rats

Rapid growth of new atmospheric particles by nitric acid and ammonia condensation

New-particle formation is a major contributor to urban smog$^{1,2}$, but how it occurs in cities is often puzzling$^{3}$. If the growth rates of urban particles are similar to those found in cleaner environments (1–10 nanometres per hour), then existing understanding suggests that new urban particles should be rapidly scavenged by the high concentration of pre-existing particles. Here we show, through experiments performed under atmospheric conditions in the CLOUD chamber at CERN, that below about +5 degrees Celsius, nitric acid and ammonia vapours can condense onto freshly nucleated particles as small as a few nanometres in diameter. Moreover, when it is cold enough (below −15 degrees Celsius), nitric acid and ammonia can nucleate directly through an acid–base stabilization mechanism to form ammonium nitrate particles. Given that these vapours are often one thousand times more abundant than sulfuric acid, the resulting particle growth rates can be extremely high, reaching well above 100 nanometres per hour. However, these high growth rates require the gas-particle ammonium nitrate system to be out of equilibrium in order to sustain gas-phase supersaturations. In view of the strong temperature dependence that we measure for the gas-phase supersaturations, we expect such transient conditions to occur in inhomogeneous urban settings, especially in wintertime, driven by vertical mixing and by strong local sources such as traffic. Even though rapid growth from nitric acid and ammonia condensation may last for only a few minutes, it is nonetheless fast enough to shepherd freshly nucleated particles through the smallest size range where they are most vulnerable to scavenging loss, thus greatly increasing their survival probability. We also expect nitric acid and ammonia nucleation and rapid growth to be important in the relatively clean and cold upper free troposphere, where ammonia can be convected from the continental boundary layer and nitric acid is abundant from electrical storms$^{4,5}$

Arctic warming by abundant fine sea salt aerosols from blowing snow

The Arctic warms nearly four times faster than the global average, and aerosols play an increasingly important role in Arctic climate change. In the Arctic, sea salt is a major aerosol component in terms of mass concentration during winter and spring. However, the mechanisms of sea salt aerosol production remain unclear. Sea salt aerosols are typically thought to be relatively large in size but low in number concentration, implying that their influence on cloud condensation nuclei population and cloud properties is generally minor. Here we present observational evidence of abundant sea salt aerosol production from blowing snow in the central Arctic. Blowing snow was observed more than 20% of the time from November to April. The sublimation of blowing snow generates high concentrations of fine-mode sea salt aerosol (diameter below 300 nm), enhancing cloud condensation nuclei concentrations up to tenfold above background levels. Using a global chemical transport model, we estimate that from November to April north of 70° N, sea salt aerosol produced from blowing snow accounts for about 27.6% of the total particle number, and the sea salt aerosol increases the longwave emissivity of clouds, leading to a calculated surface warming of +2.30 W m−2 under cloudy sky conditions