Dormancy patterns in buried seeds of <i>Datura ferox</i> and <i>D</i>. <i>stramonium</i>

Seasonal changes in seed dormancy of Datura ferox L. and D. stramonium L. were studied during 2 years in seeds buried in the field and compared with changes in dormancy of dry-stored seeds. Dormancy levels were evaluated by measuring the germination of exhumed and dry-stored seeds under 32:27 °C (nondormant seeds) or 32:12 °C (relatively dormant seeds). Levels of relative or full dormancy did not change during 2 years of dry storage in either species. Seed burial at 20 cm accelerated after-ripening, and primary dormancy disappeared earlier than at 5 cm. However, burial depths greater than 10 cm prevented germination in situ. This inhibition was not due to smaller daily fluctuations of soil temperature with increasing depth, since seeds buried at 20 cm and kept at 32:12 °C also failed to germinate. Transferring seeds to shallower soil layers allowed their germination. Cyclic seasonal changes in dormancy were found in buried seeds of D. ferox but not in D. stramonium. In D. ferox, dormancy was alleviated during the winter and germinability was maximal in early spring; the increase in dormancy was preceded by high late spring – summer temperatures. In D. stramonium most seeds near the soil surface germinated in the field in a brief flush in early winter, at temperatures under which D. ferox did not germinate. Key words: Datura, germination, dormancy, buried seeds, temperature. </jats:p

Evaluating the invasion potential of non-native tree species in Europe

Abstrakt

Genome-wide identification of DNaseI hypersensitive sites using active chromatin sequence libraries

Comprehensive identification of sequences that regulate transcription is one of the major goals of genome biology. Focal alteration in chromatin structure in vivo, detectable through hypersensitivity to DNaseI and other nucleases, is the sine qua non of a diverse cast of transcriptional regulatory elements including enhancers, promoters, insulators, and locus control regions. We developed an approach for genome-scale identification of DNaseI hypersensitive sites (HSs) via isolation and cloning of in vivo DNaseI cleavage sites to create libraries of active chromatin sequences (ACSs). Here, we describe analysis of >61,000 ACSs derived from erythroid cells. We observed peaks in the density of ACSs at the transcriptional start sites of known genes at non-gene-associated CpG islands, and, to a lesser degree, at evolutionarily conserved noncoding sequences. Peaks in ACS density paralleled the distribution of DNaseI HSs. ACSs and DNaseI HSs were distributed between both expressed and nonexpressed genes, suggesting that a large proportion of genes reside within open chromatin domains. The results permit a quantitative approximation of the distribution of HSs and classical cis-regulatory sequences in the human genome

Abundant non-native tree species in Europe: traits and effects on ecosystems

Vytauto Didžiojo universitetasŽemės ūkio akademij