Comparison of Two Lipid Extraction Methods Produced by Yeast in Cheese Whey

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)This work aimed to evaluate nine strains of yeast, previously identified as good producers of lipids in honey medium, for selecting the most suitable strain for the production of lipids in cheese whey medium and compared two well known extraction methods of lipids from the culture medium. The highest yield of total lipids was 1.27 g.L-1 produced by Cryptococcus laurentii 11. A comparison was made between the two culture media: cheese whey and liquid YEPG, and two lipid extraction methods: Bligh and Dyer and Folch et al. for C. laurentii. The experiments were performed with 2(2) full factorial design using two factors and two levels. Lipid content was higher in cheese whey and there was no difference in the extraction methods statistically. The method of Bligh and Dyer was used in preference to Folch et al. as it resulted in larger mean of total lipids.564629636Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Ecotoxicity evaluation : preparation of poly-Ɛcaprolactone and chitosan nanoparticles as carriers of thiamethoxam pesticide

The transmission of Huanglongbing (HLB) disease on citrus plants is through dissemination of the bacteria Candidatus Liberibacter ssp, by Diaphorina citri psyllid, its insect vector. Chemical control of the psyllids, and thiamethoxam (neonicotinoid insecticide) is one of the active ingredients used in the control of HLB. This insecticide is water soluble, unstable and rapidly degraded by photolysis. Pesticide nanoformulation is one of the strategies to control release of active compound as well as protection for premature degradation. Thus, studies of the effectiveness of encapsulated pesticide formulations are extremely important for enabling its use in agriculture. This study reports the encapsulation of the insecticide thiamethoxam in polymeric particles from poly-ε-caprolactone (PCL) and chitosan by double emulsion and solvent evaporation method using different concentrations of chitosan and two Pluronic (poloxamer) copolymers, F 127 and F68. These nanoparticles were characterized in terms of size, zeta potential, polydispersity, and encapsulation efficiency. The encapsulation efficiency, measured by liquid chromatography was 34%. The nanoparticles obtained from optimized conditions resulted in homogeneous and monodisperse particles with a positive superficial charge. The microalgae Raphidocelis subcapitata (bioindicator chloroficea) and microcrustacean Artemia salina, were used to evaluate the ecotoxicity of nanopesticide in comparison to pesticide already in the market. The ecotoxicity study demonstrated that nanopesticide was less toxic that commercial formulations in the studied conditions132