Soil structure and greenhouse gas production differences between row and interrow positions under no-tillage

No-tillage in Brazil is an effi cient agricultural system that improves crop productivity whilst controlling erosion caused to the soil by degradation. However, there is some concern regarding soil compaction. Our objective was to determine whether the function of soil structure in sustaining crop growth was dependent on row and interrow positions in long-term no-tillage. We took soil samples from a fi eld in a commercial farm under long-term no-tillage since 1979 on a clayey Oxisol in Southern Brazil. We assessed soil physical quality using the revised Peerlkamp technique and measured bulk density, air-fi lled porosity and air permeability of intact soil cores. Samples were incubated to assess in vitro N2O and CO2 production. The soil physical and structural properties showed consistent differences between interrow and row positions, where the properties measured were more favorable. The revised Peerlkamp technique proved as effi cient as quantitative parameters in discriminating treatment differences. Overall, soil physical conditions in the interrow were less favourable than in the row. Pore continuity did not vary as regards position. This may explain why row position did not infl uence in vitro N2O and CO2 production. Soil physical quality under no-tillage system is enhanced, at least in the short term, by superficial disturbances in the row as a result of the action of the coulters of the no-tillage seeder

The crystal structure of the Sgt1-Skp1 complex: the link between Hsp90 and both SCF E3 ubiquitin ligases and kinetochores

The essential cochaperone Sgt1 recruits Hsp90 chaperone activity to a range of cellular factors including SCF E3 ubiquitin ligases and the kinetochore in eukaryotes. In these pathways Sgt1 interacts with Skp1, a small protein that heterodimerizes with proteins containing the F-box motif. We have determined the crystal structure of the interacting domains of Saccharomyces cerevisiae Sgt1 and Skp1 at 2.8 Å resolution and validated the interface in the context of the full-length proteins in solution. The BTB/POZ domain of Skp1 associates with Sgt1 via the concave surface of its TPR domain using residues that are conserved in humans. Dimerization of yeast Sgt1 occurs via an insertion that is absent from monomeric human Sgt1. We identify point mutations that disrupt dimerization and Skp1 binding in vitro and find that the interaction with Skp1 is an essential function of Sgt1 in yeast. Our data provide a structural rationale for understanding the phenotypes of temperature-sensitive Sgt1 mutants and for linking Skp1-associated proteins to Hsp90-dependent pathways

Conhecimento de mulheres de uma Unidade Básica de Saúde da cidade de Natal/RN sobre o exame de Papanicolau Conocimiento de mujeres de una Unidad Básica de Salud de la ciudad de Natal/RN sobre el examen de Papanicolau Knowledge of women about the Paptest in a Basica Unit of Health in Natal

Trata-se de uma pesquisa descritiva quantitativa, desenvolvida em uma Unidade Básica de Saúde em Natal/RN, objetivando identificar o conhecimento de mulheres quanto à importância, à freqüência do exame de Papanicolau, bem como seus cuidados antes de realizá-lo e causas que levam mulheres a não se submeterem a tal exame. Utilizou-se uma entrevista estruturada na coleta de dados antes da consulta ginecológica, com uma amostra intencional de 120 mulheres. Os resultados mostram que as pesquisadas conhecem a importância do exame, a maioria realiza-o anualmente e, no geral, apresentam conhecimento satisfatório sobre os cuidados antes do exame. A vergonha de fazer o exame de Papanicolau e o medo do seu resultado são as principais causas atribuídas para a sua não realização. Conclui-se que os projetos educativos sejam direcionados para a importância, a freqüência e os cuidados necessários antes do exame de Papanicolau, como também, para a interação profissional-cliente durante a consulta ginecológica, visando a reduzir a vergonha e o medo dessas mulheres.<br>Se trata de una investigación descriptiva cuantitativa, desarrollada en una Unidad Básica de Salud en Natal/RN, con el objetivo de identificar el conocimiento de las mujeres en cuanto a la importancia y frecuencia del examen de Papanicolau, así como sus cuidados antes de realizárselo y causas que las llevan a no someterse a tal examen. Se utilizó una entrevista estructurada en la recolección de datos antes de la consulta ginecológica, con una muestra intencional de 120 mujeres. Los resultados muestran que las investigadas conocen la importancia del examen, la mayoría lo realiza anualmente y, en general, presentan conocimiento satisfactorio sobre los cuidados antes del examen. La vergüenza de hacerse el examen de Papanicolau y el miedo de su resultado son las principales causas atribuídas para su no realización. Se concluye que los proyectos educativos estén orientados a la importancia, frecuencia y cuidados necesarios antes del examen de Papanicolau, así como también, a la interacción profesional-cliente durante la consulta ginecológica, visando reducir la vergüenza y el miedo de esas mujeres.<br>This is a descriptive quantitative research carried out at a Basic Health Unit in Natal, State of Rio Grande do Norte, aimed at measuring the knowledge that women have on the importance of the Pap test and the frequency in which they undertake it, as well as how they prepare themselves to undertake it and the causes that lead women to not submit to it. For data collection a structured interview prior to the gynecologic consultation was used, with an intentional sample of 120 women. The results show that the surveyed women are aware of the importance of the test, that most of them undertake it annually and that they generally have satisfactory knowledge as for the care they must take prior to undertaking it. Embarrassment and fear of the results are the main causes associated with the refusal to undertake the test. It can be concluded that the educative projects are to be focused on the importance, the frequency and the care that must be taken prior to the test, as well as on the professional-client interaction during the gynecologic consultation, with the aim of reducing women's embarrassment and fear

Indicators of Soil Physical Quality: From Simplicity to Complexity

International audienceIn working with soil physics, getting new answers to the same questions is a challenge. As soil physicists, we are always hoping to find new ways of understanding such a complex soil science area. In this chapter, we will discuss some of the ways to assess soil physical quality for crop growth, using ascending complexity classification, from the simplest to the more complex soil physical indicators for crop growth