Influence of plant growth regulators, polyamines and glycerol interaction on growth and morphogenesis of carposporelings of Grateloupia cultured in vitro

The influence of the plant growth regulators 2,4-D, GA(3), BA and kinetin, and the polyamines putrescine, spermidine and spermine were tested on axenic in vitro cultures of carposporelings of Grateloupia doryphora. The auxin 2,4-D (10(-3) M) and the polyamine spermine (10(-6) M and 10(-3) M) induced a callus (disorganised cell mass that arose from the organised tissue of the carposporeling, as demonstrated by microscopic monitoring of the tissue). Putrescine and spermidine (10(-3) M) transformed the carposporelings into cell masses that produced shoots. BA (10(-3) M) and kinetin (10(-6) M and 10(-3) M) were inhibitory. In 10(-1) M glycerol-containing culture medium, which is known to induce the formation of morphogenic cell masses, the addition of GA(3) M) resulted in the inhibition of the morphogenesis (i.e. shoot emission) in the cell mass. The kinetin at 10(-6) M inhibited morphogenesis, whilst at 10(-3) M inhibited even the formation of the cell masses. The combination of glycerol (10(-1) M) and the auxin 2,4-D (10(-6) and 10(-3) M) or the polyamines putrescine, spermidine and spermine (10(-6) and 10(-3) M) resulted in a bigger size of the cell masses that led to a higher amount of shoots per cell mass than in glycerol alone.1009560,664Q3SCI

Polyamine levels in the seagrass Cymodocea nodosa

The levels of the polyamines putrescine, spermidine and spermine were analysed in different tissues of nature-collected samples of the seagrass Cymodocea nodosa. The highest accumulation of polyamines was found in the apical section of the rhizome. The putrescine/spermine ratio was highest in the senescent tissue of the leaf18417960,885Q2SCI

Correlação entre os diagnósticos citopatológicos e histopatológicos das lesões da mucosa oral após a punção aspirativa por agulha fina

OBJETIVO: Fazer um estudo retrospectivo, correlacionando os diagnósticos citopatológicos obtidos por punção aspirativa por agulha fina (PAAF) com os diagnósticos histopatológicos de lesões da região bucomaxilofacial, de pacientes que foram atendidos no Serviço de Cirurgia e Traumatologia Bucomaxilofacial do Hospital Universitário Antonio Pedro/UFF, entre maio de 1999 e maio de 2004. MÉTODO: Foram analisados os laudos cito e histopatológicos emitidos pelo Serviço de Anatomia Patológica (SAP-HUAP), de quarenta e cinco pacientes submetidos à a punção aspirativa por agulha fina (PAAF) por apresentar lesão na região bucomaxilofacial. RESULTADOS: Foi obtida uma precisão diagnóstica da citopatologia a partir da PAAF de 77,8% (35 casos), e especificidade e sensibilidade da técnica de 100%, não havendo nenhum resultado falso-positivo e falso-negativo. CONCLUSÃO: A citopatologia realizada a partir da PAAF é um método diagnóstico inicial eficiente, de baixo custo, praticamente indolor e rápido de ser realizado, que contribui para a identificação da natureza da lesão proporcionando um melhor planejamento terapêutico

Effects of temperature and irradiance on filament development of Grateloupia turuturu (Halymeniaceae, Rhodophyta)

Grateloupia turuturu Yamada is an economically valuable red alga with great potential in nutraceuticals and pharmaceuticals. Filaments of G. turuturu are of primary importance in germplasm preservation and sporeling culture, although filaments were not present in its life cycle. In this study, effects of temperature (10, 15, 20, 25, and 30 °C) and irradiance (10, 30, 60, and 90 μmol photons m−2 s−1) with photoperiod 10:14 h (light/dark) on filament development were investigated. Our results indicated that 25 °C was the optimal temperature for the formation of discoid crusts regardless of the irradiance. Conditions of 20 °C and 60 μmol photons m−2 s−1 promoted the development of discoid crusts and formation of upright thalli