Imaging of RNA in situ hybridization by atomic force microscopy

In this study we investigated the possibility of imaging internal cellular molecules after cytochemical detection with atomic force microscopy (AFM). To this end, rat 9G and HeLa cells were hybridized with haptenized probes for 28S ribosomal RNA, human elongation factor mRNA and cytomegalovirus immediate early antigen mRNA. The haptenized hybrids were subsequently detected with a peroxidase-labelled antibody and visualized with 3,3'-diaminobenzidine (DAB). The influence of various scanning conditions on cell morphology and visibility of the signal was investigated. In order to determine the influence of ethanol dehydration on cellular structure and visibility of the DAB precipitate, cells were kept in phosphate-buffered saline (PBS) and scanned under fluid after DAB development or dehydrated and subsequently scanned dry or submerged in PBS. Direct information on the increase in height of cellular structures because of internally precipitated DAB and the height of mock-hybridized cells was available. Results show that internal DAB precipitate can be detected by AFM, with the highest sensitivity in the case of dry cells. Although a relatively large amount of DAB had to be precipitated inside the cell before it was visible by AFM, the resolution of AFM for imaging of RNA–in situ hybridization signals was slightly better than that of conventional optical microscopy. Furthermore, it is concluded that dehydration of the cells has irreversible effects on cellular structure. Therefore, scanning under fluid of previously dehydrated samples cannot be considered as a good representation of the situation before dehydration.\ud \u

Determination of mercury distribution inside spent compact fluorescent lamps by atomic absorption spectrometry

In this study, spent compact fluorescent lamps were characterized to determine the distribution of mercury. The procedure used in this research allowed mercury to be extracted in the vapor phase, from the phosphor powder, and the glass matrix. Mercury concentration in the three phases was determined by the method known as cold vapor atomic absorption spectrometry. Median values obtained in the study showed that a compact fluorescent lamp contained 24.52 ± 0.4 ppb of mercury in the vapor phase, 204.16 ± 8.9 ppb of mercury in the phosphor powder, and 18.74 ± 0.5 ppb of mercury in the glass matrix. There are differences in mercury concentration between the lamps since the year of manufacture or the hours of operation affect both mercury content and its distribution. The 85.76% of the mercury introduced into a compact fluorescent lamp becomes a component of the phosphor powder, while more than 13.66% is diffused through the glass matrix. By washing and eliminating all phosphor powder attached to the glass surface it is possible to classified the glass as a non-hazardous waste

The Influence of Spatial Distance Priming on Test Anxiety and Judgments

This paper examined the effects of distance priming on test anxiety and judgment. Research suggests that individuals’ perceived distance can impact their affect and judgments, which sheds light on the principle of “distance equals safety” (Williams & Bargh, 2008). Taking an exam invokes both cognitive and emotional anxiety, such as worry, panic, and tension. It is hypothesized that the distance priming may reduce test anxiety—particularly, the emotionality aspect—as well as perceived test difficulty. The results showed that, counter to the hypotheses, there was no significant difference among the three priming groups in their emotional test anxiety or perceived test difficulty. There is a significant correlation between ACT score and cognitive test anxiety, supporting past literature that as one’s intellectual ability increases, their cognitive test anxiety decreases. Further research needs to be conducted to replicate the efficacy of the priming method by Williams and Bargh (2008) and to use more effective ways of provoking performance anxiety

De toestand van 42000 landschapselementen

In 2004 is een samenwerkingsverband van Landschapsbeheer Nederland en Alterra een methodiek ontwikkeld waarmee kleine landschapselementen zoals solitaire bomen, boomgroepen, bosjes, poelen en beekjes op een uniforme wijze in kaart kunnen worden gebracht (Oosterbaan en Pels 2004). Met deze methodiek, de Monitor Kleine kenmerken over de samenstelling, de kwaliteit en de beheerstoestand opgenomen. De resultaten worden vastgelegd in een databestand gekoppeld aan een GIS-bestand

Removal of mercury bonded in residual glass from spent fluorescent lamps

The current technologies available for recycling fluorescent lamps do not completely remove the phosphor powder attached to the surface of the glass. Consequently, the glass contains the mercury diffused through the glass matrix and the mercury deposited in the phosphor powder that has not been removed during treatment at the recycling plant. A low-cost process, with just one stage, which can be used to remove the layer of phosphor powder attached to the surface of the glass and its mercury was studied. Several stirring tests were performed with different extraction mixtures, different liquid–solid ratios, and different agitation times. The value of the initial mercury concentration of the residual glass was 2.37 ± 0.50 μg/g. The maximum extraction percentage was 68.38%, obtained by stirring for 24 h with a liquid–solid ratio of 10 and using an extraction solution with 5% of an acid mixture prepared with HCl and HNO3 at a ratio of 3:1 by volume. On an industrial scale the contact time could be reduced to 8 h without significantly lowering the percentage of mercury extracted. In fact, 64% of the mercury was extracted

Utilizing Dual Information for Moving Target Search Trajectory Optimization

Various recent events have shown the enormous importance of maritime search-and-rescue missions. By reducing the time to find floating victims at sea, the number of casualties can be reduced. A major improvement can be achieved by employing autonomous aerial systems for autonomous search missions, allowed by the recent rise in technological development. In this context, the need for efficient search trajectory planning methods arises. The objective is to maximize the probability of detecting the target at a certain time k, which depends on the estimation of the position of the target. For stationary target search, this is a function of the observation at time k. When considering the target movement, this is a function of all previous observations up until time k. This is the main difficulty arising in solving moving target search problems when the duration of the search mission increases. We present an intermediate result for the single searcher single target case towards an efficient algorithm for longer missions with multiple aerial vehicles. Our primary aim in the development of this algorithm is to disconnect the networks of the target and platform, which we have achieved by applying Benders decomposition. Consequently, we solve two much smaller problems sequentially in iterations. Between the problems, primal and dual information is exchanged. To the best of our knowledge, this is the first approach utilizing dual information within the category of moving target search problems. We show the applicability in computational experiments and provide an analysis of the results. Furthermore, we propose well-founded improvements for further research towards solving real-life instances with multiple searchers

A visual validation of the combined effect of pH and dilution on the porosity of carbon xerogels

The hypothesis about the formation of the porous structure of carbon xerogels when the pH and the dilution ratio of the precursor solution are simultaneously modified has been validated by means of scanning electron microscope analysis. The morphology of the carbon xerogels showed that the size and number of the clusters produced during the sol–gel reaction is not only influenced by the pH but also by the dilution ratio. As with a decrease in the pH value, an increase in the dilution ratio caused the formation of a small number of large clusters, leading to materials with large pores. However, depending on the values selected the effect of the pH and dilution ratio was enhanced or diminished by the effect of the other. Hence, an appropriate adjustment of these two variables allowed microporous, micro-mesoporous and micro-macroporous materials to be obtained with pore volumes that could not be achieved by modifying the pH or the dilution ratio separately.Financial support from the Ministerio de Economía y Competitividad of Spain MINECO (under Projects MAT2011-23733 and IPT-2012-0689-420000, (BES-2012-052676)) is greatly acknowledged. NRR is also grateful to MINECO for her predoctoral research grant.Peer reviewe